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  • 1
    Electronic Resource
    Electronic Resource
    The Ultrastructure of Specimens of Paramecium multimicronucleatum Impregnated with Silver by the Fernández-Galiano Method (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Transmission and scanning electron microscopy of specimens of Paramecium multimicronucleatum treated with the Rio-Hortega silver-impregnation method as modified by Fernández-Galiano demonstrate that considerable deposition of silver occurs around the kinetosomes, especially at the level of the basal plate and also at the proximal end of the kinetosome. In addition, silver is heavily deposited within the kinetodesmal fibers, in the fibrous matrix that surrounds the postciliary and transverse microtubules, in the connective structures observed between the two kinetosomes of a pair and between the kinetodesmal fiber and the anterior kinetosome, and in the trichocysts. Differences and similarities in sites of deposit when other methods of silver impregnation are employed are discussed and the particular value of the present technique in studies of ciliate systematics and phytogeny is stressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1982.tb01349.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Backscattered electron imaging of immunogold-labeled and silver-enhanced microtubules in cultured mammalian cells (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 5 (1987), S. 263-273 
    Details
    ISSN: 0741-0581
    Keywords: Immunocytochemistry ; Cell culture ; Cytoskeleton ; Scanning electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: This technique permits the visualization of microtubules in situ by employing silver-enhanced immunogold labeling and backscattered electron imagery. For best results, monolayer cultures of PtK2 cells are lysed with Triton X-100 in a microtubule stabilizing buffer, fixed with 1% glutaraldehyde, reduced with NaBH4, incubated with monoclonal antitubulin and 5-nm gold-labeled anti-IgG, silver enhanced, freeze dried, lightly coated with aluminum, and examined in an SEM equipped with a backscattered electron detector. A high contrast view of the entire microtubule complex of each cell is obtained. Microtubules in freeze-dried preparations have relatively smooth surfaces, whereas those in critical point dried preparations are more irregular or beaded. At high magnifications, an unstained inner core of each microtubule can be resolved. Backscattered electron imaging appears to be a promising technique for localizing cytoskeletal proteins and other intracellular antigens that can be labeled with immunogold and enhanced with silver.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060050306
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    Paper (German National Licenses)
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  • 3
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    Neisseria gonorrhoeae Filamentous Phage Ngo{Phi}6 Is Capable of Infecting a Variety of Gram-Negative Bacteria [Structure and Assembly] (2013)
    The American Society for Microbiology (ASM)
    Details
    Publication Date: 2013-12-31
    Description: We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriophage Ngo6 cloned into a pBluescript plasmid derivative lacking the f1 origin of replication (named pBS::6). Escherichia coli cells harboring pBS::6 were able to produce a biologically active phagemid, Ngo6fm, capable of infecting, integrating its DNA into the chromosome of, and producing progeny phagemids in, a variety of taxonomically distant Gram-negative bacteria, including E. coli , Haemophilus influenzae , Neisseria sicca , Pseudomonas sp., and Paracoccus methylutens . A derivative of pBS::6 lacking the phage orf7 gene, a positional homolog of filamentous phage proteins that mediate the interaction between the phage and the bacterial pilus, was capable of producing phagemid particles that were able to infect E. coli , Haemophilus influenzae , N. sicca , Pseudomonas sp., and Paracoccus methylutens , indicating that Ngo6 infects cells of these species using a mechanism that does not involve the Orf7 gene product and that Ngo6 initiates infection through a novel process in these species. We further demonstrate that the establishment of the lysogenic state does not require an active phage integrase. Since phagemid particles were capable of infecting diverse hosts, this indicates that Ngo6 is the first broad-host-range filamentous bacteriophage described.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
    Published by The American Society for Microbiology (ASM)
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  • 4
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    Lipid raft-dependent plasma membrane repair interferes with the activation of B lymphocytes (2015)
    Rockefeller University Press
    Details
    Publication Date: 2015-12-24
    Description: Cells rapidly repair plasma membrane (PM) damage by a process requiring Ca 2+ -dependent lysosome exocytosis. Acid sphingomyelinase (ASM) released from lysosomes induces endocytosis of injured membrane through caveolae, membrane invaginations from lipid rafts. How B lymphocytes, lacking any known form of caveolin, repair membrane injury is unknown. Here we show that B lymphocytes repair PM wounds in a Ca 2+ -dependent manner. Wounding induces lysosome exocytosis and endocytosis of dextran and the raft-binding cholera toxin subunit B (CTB). Resealing is reduced by ASM inhibitors and ASM deficiency and enhanced or restored by extracellular exposure to sphingomyelinase. B cell activation via B cell receptors (BCRs), a process requiring lipid rafts, interferes with PM repair. Conversely, wounding inhibits BCR signaling and internalization by disrupting BCR–lipid raft coclustering and by inducing the endocytosis of raft-bound CTB separately from BCR into tubular invaginations. Thus, PM repair and B cell activation interfere with one another because of competition for lipid rafts, revealing how frequent membrane injury and repair can impair B lymphocyte–mediated immune responses.
    Electronic ISSN: 1540-8140
    Topics: Biology
    Published by Rockefeller University Press
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