Publication Date:
2022-05-26
Description:
© The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 12 (2017): e0184849, doi:10.1371/journal.pone.0184849.
Description:
Diatoms are important components of marine ecosystems and contribute greatly to the
world's primary production. Despite their important roles in ecosystems, the molecular basis
of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely
unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic
method was coupled with a series of physiological and biochemical techniques to explore
oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom
Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses. A total
of 1151 proteins were detected; 122 and 56 were significantly differentially expressed
from control under N- and P-limited conditions, respectively. In N-limited cells, responsive
proteins were related to reactive oxygen species (ROS) accumulation, oxidative stress
responses and cell death, corresponding to a significant decrease in photosynthetic efficiency,
marked intracellular ROS accumulation, and caspase-mediated programmed cell
death activation. None of these responses were identified in P-limited cells; however, a significant
up-regulation of alkaline phosphatase proteins was observed, which could be the
major contributor for P-limited cells to cope with ambient P deficiency. These findings demonstrate
that fundamentally different metabolic responses and cellular regulations are
employed by the diatom in response to different nutrient stresses and to keep the cells
viable.
Description:
This study was funded by the National
Natural Science Foundation of China (41576138,
41076080, 41576138) to Dr. Jun-Rong Liang; the
Woods Hole Center for Oceans and Human Health,
National Science Foundation (OCE-1314642) to Dr.
DonaldM Anderson; the National Institute of Environmental Health Sciences (1-P01-ES021923-
01) to Dr. DonaldM Anderson; and the ERC
Advanced Award Diatomite and ANR project
DiaDomOil to Dr. Chris Bowler.
Repository Name:
Woods Hole Open Access Server
Type:
Article
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