Anmerkung: Cover -- Title Page -- Copyright -- Dedication -- Contents -- List of Contributors -- About the Series Editors -- Preface -- Part I Enabling and Improving Large-Scale Bio-production -- Chapter 1 Industrial-Scale Fermentation -- 1.1 Introduction -- 1.2 Industrial-Scale Fermentation Today -- 1.3 Engineering and Design Aspects -- 1.4 Industrial Design Examples -- 1.5 Cost Analysis for the Manufacture of Biotechnological Products -- 1.6 Influence of Process- and Facility-Related Aspects on Cost Structure -- Acknowledgments -- References -- Chapter 2 Scale-Down: Simulating Large-Scale Cultures in the Laboratory -- 2.1 Introduction -- 2.2 Heterogeneities at Large Scale and the Need for Scaling Down -- 2.3 Bioreactor Scale-Down -- 2.4 Tools to Study Cell Responses to Environmental Heterogeneities -- 2.5 Physiological Effects of Environmental Heterogeneities -- 2.6 Improvements Based on Scale-Down Studies: Bioreactor Design and Cell Engineering -- 2.7 Perspectives -- Acknowledgment -- References -- Chapter 3 Bioreactor Modeling -- 3.1 Large-Scale Industrial Fermentations: Challenges for Bioreactor Modeling -- 3.2 Bioreactors -- 3.3 Compartment and Hybrid Multizonal/Computational Fluid Dynamics Approaches for the Description of Large-Scale Bioreactor Phenomena -- 3.4 Computational Fluid Dynamics Modeling: Unstructured Continuum Approach (Euler-Euler) -- 3.5 Computational Fluid Dynamics Modeling: Structured Segregated Approach (Euler-Lagrange) -- 3.6 Conclusion -- 3.7 Outlook -- References -- Chapter 4 Cell Culture Technology -- 4.1 Introduction -- 4.2 Overview of Applications for Cell Culture Products and Tissue Engineering -- 4.3 Fundamentals -- 4.4 Bioreactors for Cell Culture -- 4.5 Downstream -- 4.6 Regulatory and Safety Issues -- 4.7 Conclusions and Outlook -- References -- Part II Getting Out More: Strategies for Enhanced Bioprocessing. , Chapter 5 Production of Fuels and Chemicals from Biomass by Integrated Bioprocesses -- 5.1 Introduction -- 5.2 Utilization of Starchy Biomass -- 5.3 Utilization of Lignocellulosic Biomass -- 5.4 Conclusions and Perspectives -- Acknowledgment -- References -- Chapter 6 Solid-State Fermentation -- 6.1 Introduction -- 6.2 Fundamentals Aspects of SSF -- 6.3 Factors Affecting Solid-State Fermentation -- 6.4 Scale-Up -- 6.5 Product Recovery -- 6.6 Bioreactor Designing -- 6.7 Kinetics and Modeling -- 6.8 Applications -- 6.9 Challenges in SSF -- 6.10 Summary -- References -- Chapter 7 Cell Immobilization: Fundamentals, Technologies, and Applications -- 7.1 Introduction -- 7.2 Fundamentals of Cell Immobilization -- 7.3 Immobilization with Support Materials -- 7.4 Self-Immobilization -- 7.5 Immobilized Cells and their Applications -- 7.6 Bioreactors for Cell Immobilization -- 7.7 Challenges and Recommendations for Future Research -- 7.8 Conclusions -- References -- Part III Molecules for Human Use: High-Value Drugs, Flavors, and Nutraceuticals -- Chapter 8 Anticancer Drugs -- 8.1 Natural Products as Anticancer Drugs -- 8.2 Anticancer Drug Production -- 8.3 Important Anticancer Natural Products -- 8.4 Prospects -- References -- Chapter 9 Biotechnological Production of Flavors -- 9.1 History -- 9.2 Survey on Today's Industry -- 9.3 Regulations -- 9.4 Flavor Production -- 9.5 Biotechnological Production of Flavors -- 9.6 Vanillin -- 9.7 2-Phenylethanol -- 9.8 Benzaldehyde -- 9.9 Lactones -- 9.10 Raspberry Ketone -- 9.11 Green Notes -- 9.12 Nootkatone -- 9.13 Future Perspectives -- References -- Chapter 10 Nutraceuticals (Vitamin C, Carotenoids, Resveratrol) -- 10.1 Introduction -- 10.2 Vitamin C -- 10.3 Carotenoids -- 10.4 Resveratrol -- 10.5 Future Perspectives -- References -- Part IV Industrial Amino Acids. , Chapter 11 Glutamic Acid Fermentation: Discovery of Glutamic Acid-Producing Microorganisms, Analysis of the Production Mechanism, Metabolic Engineering, and Industrial Production Process -- 11.1 Introduction -- 11.2 Discovery of the Glutamic Acid-Producing Bacterium C. glutamicum -- 11.3 Analysis of the Mechanism of Glutamic Acid Production by C. glutamicum -- 11.4 Metabolic Engineering of C. glutamicum for Glutamic Acid Production -- 11.5 Glutamic Acid Fermentation by Other Microorganisms -- 11.6 Industrial Process of Glutamic Acid Production -- 11.7 Future Perspectives -- References -- Chapter 12 l-Lysine -- 12.1 Uses of l-Lysine -- 12.2 Biosynthesis and Production of l-Lysine -- 12.3 The Chassis Concept: Biotin Prototrophy and Genome Reduction -- 12.4 l-Lysine Biosensors for Strain Selection and on-Demand Flux Control -- 12.5 Perspective -- References -- Part V Bio-Based Monomers and Polymers -- Chapter 13 Diamines for Bio-Based Materials -- 13.1 Introduction -- 13.2 Diamine Metabolism in Bacteria -- 13.3 Putrescine - 1,4-Diaminobutane -- 13.4 Cadaverine - 1,5-Diaminopentane -- 13.5 Conclusions and Perspectives -- References -- Chapter 14 Microbial Production of 3-Hydroxypropionic Acid -- 14.1 Introduction -- 14.2 3-HP Obtained from Native Producers -- 14.3 Synthesis of 3-HP from Glucose -- 14.4 Synthesis of 3-HP from Glycerol -- 14.5 Bridging the Gap Between Glucose and Glycerol in 3-HP Production -- 14.6 Other Strains for 3-HP Production from Glycerol -- 14.7 Limitations of 3-HP Synthesis -- 14.8 Conclusions and Future Prospects -- Acknowledgments -- References -- Chapter 15 Itaconic Acid - An Emerging Building Block -- 15.1 Background, History, and Economy -- 15.2 Biosynthesis of Itaconic Acid -- 15.3 Production Conditions for Itaconic Acid -- 15.4 Physiological Effects and Metabolism of Itaconic acid. , 15.5 Metabolic Engineering for Itaconic Acid Production -- 15.6 Outlook -- Acknowledgments -- References -- Part VI Top-Value Platform Chemicals -- Chapter 16 Microbial Production of Isoprene: Opportunities and Challenges -- 16.1 Introduction -- 16.2 The Milestones of Isoprene Production -- 16.3 Microbial Production of Isoprene: Out of the Laboratory -- 16.4 Main Challenges for Bioisoprene Production -- 16.5 Future Prospects -- Acknowledgments -- References -- Chapter 17 Succinic Acid -- 17.1 Introduction -- 17.2 Development of Succinic Acid Producers and Fermentation Strategies -- 17.3 Succinic Acid Recovery and Purification -- 17.4 Summary -- Acknowledgments -- References -- Part VII Biorenewable Fuels -- Chapter 18 Ethanol: A Model Biorenewable Fuel -- 18.1 Introduction -- 18.2 Metabolic Engineering: Design, Build, Test, Learn -- 18.3 Biomass Deconstruction -- 18.4 Closing Remarks -- Acknowledgments -- References -- Chapter 19 Microbial Production of Butanols -- 19.1 Introduction -- 19.2 A Historical Perspective of n-Butanol Production -- 19.3 ABE Fermentation -- 19.4 n-Butanol Production in Non-native Producers -- 19.5 Isobutanol Production -- 19.6 Summary and Outlook -- Acknowledgments -- References -- Index -- EULA.

Anmerkung: Cover -- Title Page -- Copyright -- Dedication -- Contents -- List of Contributors -- About the Series Editors -- Preface -- Volume 1 -- Part I Industrial Biotechnology: From Pioneers to Visionary -- Chapter 1 History of Industrial Biotechnology -- 1.1 The Beginning of Industrial Microbiology -- 1.2 Primary Metabolites and Enzymes -- 1.3 The Antibiotic Era -- 1.4 The Biotechnology Era Between 1970 and 2015 -- 1.5 How Pioneering Developments Led to Genetic Engineering -- References -- Chapter 2 Synthetic Biology: An Emerging Approach for Strain Engineering -- 2.1 Introduction -- 2.2 Basic Elements -- 2.3 Functional and Robust Modules -- 2.4 Microbial Communities -- 2.5 Conclusions and Future Prospects -- Acknowledgments -- References -- Chapter 3 Toward Genome-Scale Metabolic Pathway Analysis -- 3.1 Introduction -- 3.2 DD Method -- 3.3 Calculating Short EFMs in Genome-Scale Metabolic Networks -- 3.4 Conclusions -- Acknowledgments -- References -- Chapter 4 Cell-Free Synthetic Systems for Metabolic Engineering and Biosynthetic Pathway Prototyping -- 4.1 Introduction -- 4.2 Background -- 4.3 The Benefits of Cell-Free Systems -- 4.4 Challenges and Opportunities in Cell-Free Systems -- 4.5 Recent Advances -- 4.6 Summary -- Acknowledgments -- References -- Part II Multipurpose Bacterial Cell Factories -- Chapter 5 Industrial Biotechnology: Escherichia coli as a Host -- 5.1 Introduction -- 5.2 E. coli Products -- 5.3 Rewiring Central Metabolism -- 5.4 Alternative Carbon Sources -- 5.5 E. coli Techniques and Concerns -- 5.6 Conclusions -- References -- Chapter 6 Industrial Microorganisms: Corynebacterium glutamicum -- 6.1 Introduction -- 6.2 Physiology and Metabolism -- 6.3 Genetic Manipulation of Corynebacterium glutamicum -- 6.4 Systems Biology of Corynebacterium glutamicum -- 6.5 Application in Biotechnology -- 6.6 Conclusions and Perspectives. , References -- Chapter 7 Host Organisms: Bacillus subtilis -- 7.1 Introduction and Scope -- 7.2 Identification of Genetic Traits Pertinent to Enhanced Biosynthesis of a Value Product -- 7.3 Traits to Be Engineered for Enhanced Synthesis and Secretion of Proteinaceous Products -- 7.4 Engineering of Genetic Traits in Bacillus subtilis -- 7.5 Genome Reduction -- 7.6 Significance of Classical Strain Improvement in Times of Synthetic Biology -- 7.7 Resource-Efficient B. subtilis Fermentation Processes -- 7.8 Safety of Bacillus subtilis -- 7.9 Bacillus Production Strains on the Factory Floor: Some Examples -- Acknowledgments -- References -- Chapter 8 Host Organism: Pseudomonas putida -- 8.1 Introduction -- 8.2 Physiology and Metabolism -- 8.3 Genetic Manipulation -- 8.4 Systems Biology -- 8.5 Application in Biotechnology -- 8.6 Future Outlook -- References -- Part III Exploiting Anaerobic Biosynthetic Power -- Chapter 9 Host Organisms: Clostridium acetobutylicum/Clostridium beijerinckii and Related Organisms -- 9.1 Introduction -- 9.2 Microorganisms -- 9.3 Bacteriophages -- 9.4 ABE Fermentation of Solvent-Producing Clostridium Strains -- 9.5 Genome-Based Comparison of Solvent-Producing Clostridium Strains -- 9.6 Regulation of Solvent Formation in C. acetobutylicum -- 9.7 Genetic Tools for Clostridial Species -- 9.8 Industrial Application of ABE Fermentation -- Acknowledgments -- References -- Chapter 10 Advances in Consolidated Bioprocessing Using Clostridium thermocellum and Thermoanaerobacter saccharolyticum -- 10.1 Introduction -- 10.2 CBP Organism Development Strategies -- 10.3 Plant Cell Wall Solubilization by C. thermocellum -- 10.4 Bioenergetics of C. thermocellum Cellulose Fermentation -- 10.5 Metabolic Engineering -- 10.6 Summary and Future Directions -- Acknowledgments -- References -- Chapter 11 Lactic Acid Bacteria -- 11.1 Introduction. , 11.2 Fermented Foods -- 11.3 Industrially Relevant Compounds -- 11.4 Conclusions -- Conflict of Interest -- References -- Volume 2 -- Part IV Microbial Treasure Chests for High-Value Natural Compounds -- Chapter 12 Host Organisms: Myxobacterium -- 12.1 Introduction into the Myxobacteria -- 12.2 Phylogeny and Classification -- 12.3 Physiology -- 12.4 Growth and Nutritional Requirements -- 12.5 Genetics and Genomics -- 12.6 Secondary Metabolism -- 12.7 Myxococcus -- 12.8 Sorangium -- 12.9 Outlook -- References -- Chapter 13 Host Organism: Streptomyces -- 13.1 Introduction -- 13.2 Streptomyces Genome Manipulation Toolkits -- 13.3 Hosts for Heterologous Production of Natural Products -- Acknowledgments -- References -- Part V Extending the Raw Material Basis for Bioproduction -- Chapter 14 Extreme Thermophiles as Metabolic Engineering Platforms: Strategies and Current Perspective -- 14.1 Introduction -- 14.2 Bioprocessing Advantages for Extremely Thermophilic Hosts -- 14.3 Biobased Chemicals and Fuels: Targets and Opportunities -- 14.4 Considerations for Selecting an Extremely Thermophilic Host -- 14.5 General Strategies for Genetic Manipulation of Extreme Thermophiles -- 14.6 Limitations and Barriers to Genetic Modification of Extreme Thermophiles -- 14.7 Current Status of Metabolic Engineering Efforts and Prospects in Extreme Thermophiles -- 14.8 Metabolic Engineering of Extreme Thermophiles - Tool Kit Needs -- 14.9 Conclusions and Future Perspectives -- Acknowledgments -- References -- Chapter 15 Cyanobacteria as a Host Organism -- 15.1 Introduction and Relevance: Cyanobacteria as a Host Organism -- 15.2 General Description of Cyanobacteria -- 15.3 Genetic Tools -- 15.4 Improving Photosynthetic Efficiency -- 15.5 Direct Conversion of CO2 into Biofuels and Chemicals -- 15.6 Conclusions -- References -- Chapter 16 Host Organisms: Algae. , 16.1 Introduction to Algae as an Industrial Organism -- 16.2 Algal Genetic Engineering -- 16.3 Therapeutic and Nutraceutical Applications -- 16.4 Bioenergy Applications -- 16.5 Other Industrial Applications -- 16.6 Industrial-Scale Algal Production -- 16.7 Conclusions and Potential of Algal Platforms -- References -- Part VI Eukaryotic Workhorses: Complex Cells Enable Complex Products -- Chapter 17 Host Organisms: Mammalian Cells -- 17.1 Introduction -- 17.2 Basics of Cellular Structure and Metabolism -- 17.3 The Genome of CHO Cells -- 17.4 Molecular Biology Tools -- 17.5 Kinetics of Growth and Product Formation -- 17.6 Intracellular Metabolome Analysis -- 17.7 Proteome and Gene Expression Analysis -- 17.8 Improving Cellular Performance by Genetic and Metabolic Engineering -- 17.9 Outlook -- References -- Chapter 18 Industrial Microorganisms: Saccharomyces cerevisiae and other Yeasts -- 18.1 Industrial Application of Yeasts -- 18.2 Baker's Yeast as Versatile Host for Metabolic Engineering -- 18.3 Protein Production in Yeasts -- 18.4 Lipid Production in Yeasts -- 18.5 Pentose-Utilizing Yeasts -- 18.6 Conclusions -- Conflict of Interest -- References -- Chapter 19 Industrial Microorganisms: Pichia pastoris -- 19.1 Physiology and Genetics of Pichia pastoris -- 19.2 Methylotrophic Metabolism -- 19.3 Application for the Production of Recombinant Proteins -- 19.4 Application of P. pastoris for Metabolite Production -- 19.5 Conclusion -- References -- Index -- EULA.