Publikationsdatum:
2017-12-15
Beschreibung:
ABSTRACT Sodium nitrite (NaNO2) is widely used as a food additive and preservative in fish and meat products. We have evaluated the effect of a single acute oral dose of NaNO 2 on oxidative stress parameters, antioxidant capacity and DNA in rat kidney. Male Wistar rats were divided into four groups and given single oral dose of NaNO 2 at 20, 40, 60 and 75 mg/kg body weight; untreated rats served as the control group. All animals in NaNO 2 -treated groups showed marked alterations in various parameters of oxidative stress as compared to the control group. This included increase in lipid peroxidation, protein oxidation, hydrogen peroxide levels and decrease in reduced glutathione content and antioxidant capacity. Administration of NaNO 2 also increased DNA damage as evident from release of free nucleotides and confirmed by comet assay. It also led to greater cross-linking of DNA to proteins. Histological analysis showed marked morphological changes in the kidney of NaNO 2 -treated animals. These alterations could be due to increased free radical generation or direct chemical modification by reaction intermediates. Our results suggest that nitrite-induced nephrotoxicity is mediated through redox imbalance and results in DNA damage. This article is protected by copyright. All rights reserved
Digitale ISSN:
0091-7419
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
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