In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 4 ( 2022-4-14), p. e0264207-
Abstract:
Bioplastics, synthesized by several microbes, accumulates inside cells under stress conditions as a storage material. Several microbial enzymes play a crucial role in their degradation. This research was carried to test the biodegradability of poly-β-hydroxybutyrate (PHB) utilizing PHB depolymerase, produced by bacteria isolated from sewage waste soil samples. Potent PHB degrader was screened based on the highest zone of hydrolysis followed by PHB depolymerase activity. Soil burial method was employed to check their degradation ability at different incubation periods of 15, 30, and 45 days at 37±2°C, pH 7.0 at 60% moisture with 1% microbial inoculum of Aeromonas caviae Kuk1-(34) (MN414252). Without optimized conditions, 85.76% of the total weight of the PHB film was degraded after 45 days. This degradation was confirmed with Fourier-transform infrared spectroscopy (FTIR) and Scanning electron microscope (SEM) analysis. The presence of bacterial colonies on the surface of the degraded film, along with crest, holes, surface erosion, and roughness, were visible. Media optimization was carried out in statistical mode using Plackett Burman (PB) and Central Composite Design (CCD) of Response Surface Methodology (RSM) by considering ten different factors. Analysis of Variance (ANOVA), Pareto chart, response surface plots, and F-value of 3.82 implies that the above statistical model was significant. The best production of PHB depolymerase enzyme (14.98 U/mL) was observed when strain Kuk1-(34) was grown in a media containing 0.1% PHB, K 2 HPO 4 (1.6 gm/L) at 27 ℃ for seven days. Exploiting these statistically optimized conditions, the culture was found to be a suitable candidate for the management of solid waste, where 94.4% of the total weight of the PHB film was degraded after 45 days of incubation.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0264207
DOI:
10.1371/journal.pone.0264207.g001
DOI:
10.1371/journal.pone.0264207.g002
DOI:
10.1371/journal.pone.0264207.g003
DOI:
10.1371/journal.pone.0264207.g004
DOI:
10.1371/journal.pone.0264207.g005
DOI:
10.1371/journal.pone.0264207.g006
DOI:
10.1371/journal.pone.0264207.g007
DOI:
10.1371/journal.pone.0264207.t001
DOI:
10.1371/journal.pone.0264207.t002
DOI:
10.1371/journal.pone.0264207.t003
DOI:
10.1371/journal.pone.0264207.t004
DOI:
10.1371/journal.pone.0264207.t005
DOI:
10.1371/journal.pone.0264207.t006
DOI:
10.1371/journal.pone.0264207.t007
DOI:
10.1371/journal.pone.0264207.t008
DOI:
10.1371/journal.pone.0264207.t009
DOI:
10.1371/journal.pone.0264207.t010
DOI:
10.1371/journal.pone.0264207.t011
DOI:
10.1371/journal.pone.0264207.t012
DOI:
10.1371/journal.pone.0264207.s001
DOI:
10.1371/journal.pone.0264207.s002
DOI:
10.1371/journal.pone.0264207.s003
DOI:
10.1371/journal.pone.0264207.s004
DOI:
10.1371/journal.pone.0264207.r001
DOI:
10.1371/journal.pone.0264207.r002
DOI:
10.1371/journal.pone.0264207.r003
DOI:
10.1371/journal.pone.0264207.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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