In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 9 ( 2020-09-01), p. 4859-4866
Kurzfassung:
Strain CA7 T , a Gram-stain-negative, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, was isolated from raw cow’s milk collected from a farm affiliated with Chung-Ang University, Anseong, Korea, and characterized by a polyphasic approach. Optimal growth of strain CA7 T was observed on tryptic soy agar at 30 °C and pH 7.0 with 0 % NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CA7 T belonged to the genus Chryseobacterium . The most closely related strains (16S rRNA gene sequence similarity indicated in parentheses), based on the phylogenetic analysis, were Chryseobacterium rhizosphaerae KCTC 22548 T (98.08 %), Chryseobacterium nakagawai CCUG 60563 T (98.61 %), Chryseobacterium jejuense KACC 12501 T (97.85 %) and Chryseobacterium aurantiacum KCTC 62135 T (97.78 %). Whole genome sequencing indicated that the genome size was 5 125 723 bp and had a DNA G+C content of 37.4 mol%. Average nucleotide identity values for strain CA7 T with C. rhizosphaerae , C. nakagawai , C. jejuense , C. aurantiacum , and the type species of the genus Chryseobacterium, C. gleum, were 80.2, 79.8, 79.8, 79.6 and 80.4 %, respectively. The digital DNA–DNA hybridization values of CA7 T compared to C. rhizosphaerae , C. nakagawai , C. jejuense , C. aurantiacum and C. gleum were 24.1, 23.9, 23.9, 23.7 and 24.3 %, respectively. The major fatty acids were iso-C 15 : 0 , summed feature 9 (iso-C 17 : 1 ω 9 c and/or C 16 : 0 10-methyl), iso-C 17 : 0 3-OH and summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω 7 c ). Menaquinone-6 was the only respiratory quinone. The major polar lipid was phosphatidylethanolamine. Based on this polyphasic taxonomic study, strain CA7 T represents a novel species of the genus Chryseobacterium for which the name Chryseobacterium vaccae sp. nov. is proposed. The type strain is CA7 T (=KACC 21402 T =JCM 33749 T ).
Materialart:
Online-Ressource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004250
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2020
ZDB Id:
215062-1
ZDB Id:
2056611-6
SSG:
12
Permalink