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  • 1
    Digitale Medien
    Digitale Medien
    The use of bulked segregant analysis to accumulate RAPD markers near a locus for beet cyst nematode resistance in Beta vulgaris (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Bulked segregant analysis (BSA) was used to accumulate RAPD markers near the beet cyst nematode resistance locus Hslpro-1 of sugar beet (Beta vulgaris L.). Graphical genotypes constructed from RFLP data were utilized to select F2 individuals in (1) the construction of pools of plants used in the initial screening for polymorphisms, and (2) the selection of individual plants used to confirm the potential linkage. The pooled DNA samples were screened for polymorphisms using 668 RAPD primers. Forty-four candidate markers potentially linked to the region were analysed further using 14 segregating individuals. Close linkage was confirmed for 17 of the markers. Four of the RAPD markers were assigned map coordinates within the RFLP map. Three of these markers extended the RFLP map by 3cM. Altogether, the 8cM target interval contains 10 RFLP and 17 RAPD markers, corresponding to an average marker density of 0.3cM in the Hslpro-1 region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1997.tb00970.x
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Competition as a source of errors in RAPD analysis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1185-1192 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Random amplified polymorphic DNA ; Competition ; DNA mixtures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have used artificial 1∶1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1∶1 DNA mixture. In 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the RAPD reaction. Thus, RAPD markers will lead to an erroneous genetic interpretation in 14% of all cases. In contrast, the formation of non-parental heteroduplex bands was found at a frequency of only 0.2%. Analysis of 1∶ 1 mixtures using (1) a different set of optimized reaction conditions and (2) a material with low genomic complexity (Bacillus cereus) gave identical results. Serial dilutions of one genome into another, in steps of 10%, showed that all of the polymorphic bands decreased in intensity as a linear function of their respective proportion in the mixture. In dilutions with water no differences in band intensity were detected. Thus, competition occurs in the amplification of all RAPD fragments and is a major source of genotyping errors in RAPD analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223449
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Competition as a source of errors in RAPD analysis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1185-1192 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Random amplified polymorphic DNA  ;  Competition  ;  DNA mixtures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have used artificial 1:1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1:1 DNA mixture. In 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the RAPD reaction. Thus, RAPD markers will lead to an erroneous genetic interpretation in 14% of all cases. In contrast, the formation of non-parental heteroduplex bands was found at a frequency of only 0.2%. Analysis of 1: 1 mixtures using (1) a different set of optimized reaction conditions and (2) a material with low genomic complexity (Bacillus cereus) gave identical results. Serial dilutions of one genome into another, in steps of 10%, showed that all of the polymorphic bands decreased in intensity as a linear function of their respective proportion in the mixture. In dilutions with water no differences in band intensity were detected. Thus, competition occurs in the amplification of all RAPD fragments and is a major source of genotyping errors in RAPD analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050355
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    RFLP markers in the genus Beta: characterization of DNA sequences from a Beta vulgaris library (1994)
    Springer
    Genetica 92 (1994), S. 91-99 
    Details
    ISSN: 1573-6857
    Schlagwort(e): Beta ; sugar beet ; restriction fragment length polymorphism ; hybridization stringency
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract DNA sequences from a sugar beet genomic library were tested for their ability to crosshybridize and detect RFLPs in 40 accessions representing 13 different species and subspecies. A set of 32 sugar beet DNA sequences was categorized as either single copy non-polymorphic (SCN), single copy polymorphic (SCP), low copy repeated (LCR), or high copy repeated (HCR). The crosshybridization of these DNA sequences was analyzed at two different stringencies. At medium stringency, 12 of the DNA sequences hybridized exclusively to accessions from the section Beta, whereas 16 also crosshybridized to accessions from the section Procumbentes. Accessions from the related genus Atriplex showed crosshybridization for 13 of the DNA sequences. At low stringency, 94% of the sugar beet SCP sequences crosshybridized to the Procumbentes accessions, indicating that most of these sequences will crosshybridize with the majority of the species in the genus Beta. High levels of polymorphisms among the wild beets from the section Beta indicate that marker-guided backcross programmes are feasible using sugar beet genomic clones. The sugar beet DNA sequences also detected a considerable amount of RFLP variation among the Procumbentes and Atriplex accessions. This reflects the general usefulness of these markers in comparative genome mapping projects within the genus Beta. In another experiment, 87 clones were used in Northern hybridizations to estimate the proportion of transcribed sequences present in the genomic library. In total, 17 clones detected transcripts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00163757
    Standort Signatur Einschränkungen Verfügbarkeit
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