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  • 1
    Electronic Resource
    Electronic Resource
    Optimization of rice .alpha.-amylase production using temperature-sensitive mutants of Saccharomyces cerevisiae for the PHO regulatory system (1995)
    s.l. : American Chemical Society
    Biotechnology progress 11 (1995), S. 510-517 
    Details
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bp00035a003
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  • 2
    Electronic Resource
    Electronic Resource
    Phthalate Degradation in Pseudomonas putida (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 613 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb18263.x
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  • 3
    Electronic Resource
    Electronic Resource
    Constant copy numbers of plasmids inSaccharomyces cerevisiae hosts with different ploidies (1985)
    Springer
    Applied microbiology and biotechnology 23 (1985), S. 123-129 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The cellular β-galactosidase activities produced by thelac'Z gene ofEscherichia coli, cloned on YEp, YRp, or YCp-type plasmids in host cells ofSaccharomyces cerevisiae with different ploidies, and which was expressed by a modified jeastHIS5 promoter, showed characteristic differences depending on the plasmid. But for any given plasmid, the isogenic diploid and tetraploid transformants showed slightly lower enzyme activities than their respective haploid transformants. This was due to the similar copy numbers of the plasmids in host cells. Since the cell number per unit volume of the culture decreased with increasing cell ploidy, the enzyme activity per unit volume of the culture decreased significantly. The holding stability of plasmids increased with increasing ploidy of the host cell, especially that of the YRp plasmid. On the YRp plasmid, thelac'Z gene showed higher productivity withTRP1 thanLEU2 as the selection marker for the plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00938964
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  • 4
    Electronic Resource
    Electronic Resource
    Constant copy numbers of plasmids inSaccharomyces cerevisiae hosts with different ploidies (1985)
    Springer
    Applied microbiology and biotechnology 23 (1985), S. 123-129 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The cellular β-galactosidase activities produced by thelac'Z gene ofEscherichia coli, cloned on YEp, YRp, or YCp-type plasmids in host cells ofSaccharomyces cerevisiae with different ploidies, and which was expressed by a modified jeastHIS5 promoter, showed characteristic differences depending on the plasmid. But for any given plasmid, the isogenic diploid and tetraploid transformants showed slightly lower enzyme activities than their respective haploid transformants. This was due to the similar copy numbers of the plasmids in host cells. Since the cell number per unit volume of the culture decreased with increasing cell ploidy, the enzyme activity per unit volume of the culture decreased significantly. The holding stability of plasmids increased with increasing ploidy of the host cell, especially that of the YRp plasmid. On the YRp plasmid, thelac'Z gene showed higher productivity withTRP1 thanLEU2 as the selection marker for the plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982728
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  • 5
    Electronic Resource
    Electronic Resource
    Construction of a promoter-probe vector with thePHO5 gene encoding repressible acid phosphatase inSaccharomyces cerevisiae (1988)
    Springer
    Applied microbiology and biotechnology 28 (1988), S. 155-159 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A YCp type promoter-probe vector, pVC701, replicable inSaccharomyces cerevisiae andEscherichia coli hosts was constructed. pVC701 has a DNA fragment bearing thePHO5 gene encoding repressible acid phosphatase (rA-Pase; EC 3.1.3.2.) without its promoter region. The clonedPHO5 gene can be expressed by insertion of a DNA fragment having promoter function at theEcoRI site on the 5′-flanking region ofPHO5. rAPase activity caused by thePHO5 expression is easily detected by staining the transformant colonies with diazo-coupling reagent. These were confirmed by insertion of aHIS5 DNA fragment ofS. cerevisiae having promoter function at theEcoRI cloning site in conditions of histidine starvation. Numerous DNA fragments exhibiting promoter function were isolated by employing pVC701. Most of them expressed thePHO5 gene constitutively, while one of them conferred galactose-inducible and glucose-repressible expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694304
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  • 6
    Electronic Resource
    Electronic Resource
    Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae (1996)
    Springer
    Current genetics 29 (1996), S. 344-351 
    Details
    ISSN: 1432-0983
    Keywords: Key words Arsenate resistance ; Phosphatase regulation ; Pi-transporter ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The PHO84 gene in Saccharomyces cerevisiae encodes a Pi transporter, mutation of which confers constitutive synthesis of repressible acid phosphatase (rAPase), in medium containing repressible amounts of Pi, and an arsenate-resistant phenotype. We selected an arsenate-resistant mutant showing the constitutive synthesis of rAPase on nutrient plates containing 4.5 mM arsenate. This mutant has double mutations designated as pho86 and pho87. The mutant transcribes PHO84 even in the repressible condition but has a severe defect in Pi uptake. The constitutive rAPase+ phenotype of the pho86 pho87 mutant was partially suppressed by an increased dosage of the PHO84 gene. The PHO87 gene was found to be identical with YCR524, according to the published nucleotide sequence of chromosome III, which encodes a protein of 923 amino-acid residues with a highly charged N-terminal half followed by a C-terminal half consisting of 12 membrane-spanning segments as in Pho84p. These and the other findings suggest that the Pho86p and Pho87p proteins collaborate with Pho84p in Pi uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050055
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  • 7
    Electronic Resource
    Electronic Resource
    Two new genes,PHO86 andPHO87, involved in inorganic phosphate uptake inSaccharomyces cerevisiae (1996)
    Springer
    Current genetics 29 (1996), S. 344-351 
    Details
    ISSN: 1432-0983
    Keywords: Arsenate resistance ; Phosphatase regulation ; Pi-transporter ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract ThePHO84 gene inSaccharomyces cerevisiae encodes a Pi transporter, mutation of which confers constitutive synthesis of repressible acid phosphatase (rAPase), in medium containing repressible amounts of Pi, and an arsenate-resistant phenotype. We selected an arsenate-resistant mutant showing the constitutive synthesis of rAPase on nutrient plates containing 4.5 mM arsenate. This mutant has double mutations designated aspho86 andpho87. The mutant transcribesPHO84 even in the repressible condition but has a severe defect in Pi uptake. The constitutive rAPase+ phenotype of thepho86 pho87 mutant was partially suppressed by an increased dosage of thePHO84 gene. ThePHO87 gene was found to be identical withYCR524, according to the published nucleotide sequence of chromosome III, which encodes a protein of 923 amino-acid residues with a highly charged N-terminal half followed by a C-terminal half consisting of 12 membrane-spanning segments as in Pho84p. These and the other findings suggest that the Pho86p and Pho87p proteins collaborate with Pho84p in Pi uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208615
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  • 8
    Electronic Resource
    Electronic Resource
    A putative membrane protein, Pho88p, involved in inorganic phosphate transport in Saccharomyces cerevisiae (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 580-590 
    Details
    ISSN: 1617-4623
    Keywords: Key words Membrane proteins ; Phosphatase regulation ; Pho88p ; Pi transporter ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transcription of a regulatory gene, PHO81, in the phosphatase regulon of Saccharomyces cerevisiae is repressed by inorganic phosphate (Pi) in the medium via that same regulatory system. The activity of Pho81p, the product of PHO81, is also inhibited by a high concentration of Pi in the medium. Increased dosage of PHO86, a gene encoding a putative membrane protein associated with a Pi transporter complex, activates the Pi-inhibited Pho81p produced under the control of the GAL1 promoter. A new gene, PHO88/ YBR106w, has now been identified as a multicopy suppressor of the rAPase- phenotype of the cells caused by the P i inhibition of Pho81p. The pho86 disruptant expressed rAPase activity in high-Pi medium, while the pho88 disruptant did not. The Δpho86Δpho88 double disruption resulted in enhanced synthesis of rAPase under the high-Pi condition and conferred arsenate resistance on the cells than those in single disruptants of these genes. Its hydropathy profile and the results of an analysis of its cellular localization suggested that Pho88p is a membrane protein similar to Pho86p. Both disruption and high dosage of PHO88 or PHO86 resulted in reduced Pi uptake. These findings suggest that Pho88p is also involved in Pi transport and modulates Pho81p function together with Pho86p.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173648
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  • 9
    Electronic Resource
    Electronic Resource
    Two-spored asci produced by interrupted sporulation: A novel approach to linkage analysis in yeast (1983)
    Springer
    Molecular genetics and genomics 191 (1983), S. 165-166 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetical analysis of two-spored asci formed by interrupted sporulation offers a novel procedure for mapping of centromere-linked genes in Saccharomyces cerevisiae. Unlike the two-spored asci encountered under normal sporulation conditions, these asci are produced by a nonrandom mechanism. They fall into three categories (++), (+-) and (--) with respect to any marker. The percentage of (+-) asci varies directly as a function of centromere-linkage of a gene. It is observed that almost 100% asci are of the (+-) type in case of very tightly linked genes like trp-1 and cdc-10, while in case of markers unlinked to the centromere, e. g, trp-5 and met-8, the (+-) asci constitute 50% of the total number of asci. Other markers with varying degrees of linkage, e. g. ura-3 and lys-1 show corresponding numbers of (+-) asci between 50% and 100% of the total asci. These findings are in contrast to the results expected from a random abortion of two spores, in which case the (+-) asci would constitute 67% of the total number of asci irrespective of the degree of centromere linkage of a marker. The linkage-dependent segregation of markers in these new kind of two-spored asci permits a rapid and accurate estimate of centromere linkage of a gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330906
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  • 10
    Electronic Resource
    Electronic Resource
    A mutant plasmid with increased stability of holding and polymerization in Saccharomyces cerevisiae (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 14-20 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A yeast mutant plasmid, pX, showing increased stability of holding in mitotic and meiotic cell division, was isolated from an unstable plasmid, YRp7, which consists of pBR322 and a 1.4 kilobase (kb) fragment of Saccharomyces cerevisiae carrying the TRP1 gene and the autonomously replicating sequence, ARS1. The pX plasmid exists as circular molecules in a series of polymeric forms from the monomer to the 20-mer or more, consisting, except for the monomer, of even numbers of unit molecules in tandem arrangement. The pX monomer consists solely of a yeast DNA fragment of 849 base pairs (bp) containing the TRP1 and ARS1 sequences derived from the 1.4 kb yeast fragment of YRp7. The copy number of pX was calculated to be 20 as monomer units per genome. The ARS1 region was delimited to 80 bp by this mutation and the region essential for the ARS function was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327503
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