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  • 1
    Online Resource
    Online Resource
    Cham :Springer International Publishing AG,
    Keywords: Algae-Physiology. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (534 pages)
    Edition: 1st ed.
    ISBN: 9783030333973
    Series Statement: Advances in Photosynthesis and Respiration Series ; v.45
    Language: English
    Note: Intro -- Copyright -- From the Series Editors -- Authors of Volume 45 -- Our Books -- Series Editors -- Contents -- Preface: A Brief Introduction to the Algae -- The Evolution of Photosynthesis -- The Evolution of Algae -- The Evolution of Eukaryotic Algae -- About the Editors -- Contributors -- Author Index -- Part I: Introductory Chapters -- Chapter 1: Recent Advances in the Photosynthesis of Cyanobacteria and Eukaryotic Algae -- I. Algal Systematics -- II. Cyanobacteria -- III. Crystal Structures -- IV. Light Harvesting -- V. Photoinhibition -- VI. Dinoflagellates and Coral Bleaching -- VII. Carbon Uptake and Metabolism (See Chap. 7 & -- 8) -- VIII. Water-Water Cycles (See Chap. 8) -- References -- Chapter 2: The Algal Tree of Life from a Genomics Perspective -- I. Introduction -- II. Why Inferring the Algal Tree of Life Is Non-trivial -- III. Examples of Reticulate Behavior Among Algal Genes -- IV. From Designer Datasets to Whole Genomes -- V. Conclusions -- Acknowledgments -- References -- Part II: Molecular Genetics of Algae -- Chapter 3: Chlorophyll-Xanthophyll Antenna Complexes: In Between Light Harvesting and Energy Dissipation -- I. Introduction -- II. Chromophores -- III. The Core Complexes of PSII and PSI -- IV. Light Harvesting -- A. Type I (LHCBM3, 4, 6, 8 and 9) -- B. Type II (LHCBM5) -- C. Type III (LHCBM2 and 7) -- D. Type IV (LHCBM1) -- V. Antenna Complexes of PSI -- VI. Fucoxanthin Chlorophyll Binding Proteins -- VII. Photoprotection -- VIII. Triggers of Quenching Reactions -- IX. Conclusions -- Acknowledgements -- References -- Chapter 4: The Dynamics of the Photosynthetic Apparatus in Algae -- I. Introduction -- II. Adaptation to Changes in Light Conditions -- A. State Transitions -- B. Non Photochemical Quenching (NPQ) -- C. PSII Repair Cycle. , III. Response of the Photosynthetic Apparatus to Micronutrient Depletion -- A. Copper Deficiency -- B. Iron Deficiency -- C. Sulfur Deprivation and Hydrogen Production -- D. Nitrogen Deprivation -- IV. Long Term Response: Changes in Nuclear and Chloroplast Gene Expression -- V. Conclusions and Perspectives -- Acknowledgements -- References -- Chapter 5: Biosynthesis of Chlorophyll and Bilins in Algae -- I. Introduction -- II. Diversity of Chlorophylls in Algae -- III. Diversity of Bilins in Algae -- IV. Overview of Biosynthesis of Bilins and Chlorophylls -- V. Biosynthesis of Protoporphyrin IX -- VI. Biosynthesis of Bilins from Protoporphyrin and Function of Bilin Lyases -- VII. Biosynthesis of Chlorophylls from Protoporphyrin IX -- VIII. Synthesis of Chlorophyll b, d and f -- IX. Concluding Remarks -- Bibliography -- Part III: Biochemistry and Physiology of Algae -- Chapter 6: Chloroplast Ion and Metabolite Transport in Algae -- I. Introduction -- II. Chloroplast Ion Transport -- A. Ion Channels -- 1. Voltage-Dependent Chloride Channels -- 2. Mechanosensitive Ion Channels -- 3. K+ Channels -- 4. Ca2+ Channels -- B. Ion Transporters -- 1. Phosphate Transporters -- 2. Sulfate Transporters -- 3. Nitrite Transporters -- 4. Potassium Proton Exchangers -- 5. Manganese and Calcium Transporters -- 6. Magnesium Transporters -- 7. Iron Transporters -- C. Ion Pumps (P-ATPases) -- D. ABC Transporters -- III. Chloroplast Metabolite Transport -- A. ATP Transporters -- 1. Plastidic Nucleotide Translocators -- 2. The Thylakoid ATP/ADP Carrier -- B. Plastidic Phosphate Transporters -- 1. Triose-Phosphate Transporters -- 2. Phosphoenolpyruvate Transporters -- 3. Glucose-6-Phosphate and Xylulose-5-Phosphate Translocators -- C. Bicarbonate Transporters -- D. Organic Acid Transporters -- E. Amino Acid Transporters -- F. Fatty Acid Transporters. , G. Lipid ABC Transporters -- IV. Strategies for Identification of Missing Algal Transporters -- V. Conclusions and Perspectives -- References -- Chapter 7: Structural and Biochemical Features of Carbon Acquisition in Algae -- I. Introduction -- II. Carbon Assimilation -- A. The Characteristics of Most Rubiscos Necessitate Operation of a CCM -- B. The PCRC and Other Pathways for C Assimilation -- III. Occurrence of CCMs -- IV. Mechanisms of CCMs Versus Diffusive CO2 Fluxes -- A. Definition of CCMs and What Do We Need in Order to Demonstrate Operation of CCMs? -- B. CCMs Based on Active Transport of Inorganic C Species -- C. C4 Photosynthesis as a CCM in Algae? -- V. Structural Aspects of CO2 Acquisition -- Acknowledgements -- References -- Chapter 8: Light-Driven Oxygen Consumption in the  Water-Water Cycles and Photorespiration, and Light Stimulated Mitochondrial Respiration -- I. Introduction -- II. The Evidence of Light-Dependent O2 Uptake -- III. Possible Mechanisms of Light-Driven O2 Uptake -- A. Water-Water Cycles -- B. The Mehler Ascorbate Peroxidase (MAP) Reactions Involving PSI and PSII -- C. Flavodiiron Protein Involving PSI and PSII -- D. Plastid (Plastoquinol) Terminal Oxidase (PTOX) Involving PSII but not PSI -- E. Photorespiration -- F. Mitochondrial Respiration -- G. Allocation of O2 Uptake Among the Five Pathways -- IV. Functions of the Light-Driven O2 Uptake Processes -- V. Conclusions -- Acknowledgements -- References -- Chapter 9: The Algal Pyrenoid -- I. Introduction -- A. A Pyrenoid Timeline - From Microscopic Curiosity to a Key Factor in the Earth's Carbon Cycle -- B. Pyrenoid Prevalence -- C. Independent Origins but Convergent Structures -- D. Diversity -- II. Pyrenoid Structure & -- Function: Lessons from Chlamydomonas -- A. Structure and Organisation. , B. Functional Integration of Pyrenoid Proteome and CCM Activity -- C. Integrating Transcriptomics and Pyrenoid-Associated Processes -- III. When, Where, How and Whither: From Paleo-Origins to Future Synthetic Biology -- References -- Part IV: Light-Harvesting Systems in Algae -- Chapter 10: Light-Harvesting in Cyanobacteria and Eukaryotic Algae: An Overview -- I. Introduction -- II. The Photosynthetic Pigments of Cyanobacteria and Eukaryotic Algae -- A. Chlorophylls -- 1. Chlorophyll a -- 2. Chlorophyll b -- 3. Chlorophyll c and MgDVD -- 4. Chlorophyll d -- 5. Chlorophyll f -- 6. Summary Comments on the Chlorophylls -- B. Carotenoids -- 1. Carotenes -- 2. Xanthophylls -- C. Phycobiliproteins -- III. The Evolution of Protists with Plastids (Algae) -- A. Algae with Primary Plastids -- 1. Glaucophyceae -- 2. Rhodophyceae -- 3. Chlorophyceae -- B. Secondary and Tertiary Plastids -- 1. Diatoms (Bacillariophyceae) and Related Phyla Including the Phaeophyceae -- 2. Related Phyla -- 3. Dinoflagellates -- 4. Cryptophytes (Cryptophyceae) -- 5. Other Stramenopiles, Haptophytes and Apicomplexans -- IV. The Need for Light-Harvesting Antennas -- V. Light-Harvesting Antennas in Cyanobacteria and Eukaryotic Algae -- VI. Control of Energy Supply to PSI and PSII: State Transitions, Absorption Cross-Sectional Changes and Spillover -- A. Overview -- B. State Transitions -- 1. State Transitions in Chlamydomonas -- 2. An Aside on Cyclic Electron Transport (CET) -- 3. State Transitions in Other Algae -- C. Absorption Cross-Sectional Changes -- D. Spillover -- E. Complementary Chromatic Adaptation -- F. Non-photochemical Quenching - Sensu Lato -- VII. Non-photochemical Quenching -- A. The Xanthophyll Cycle -- B. pH Quenching -- C. Orange Carotenoid Protein -- VIII. Reactive Oxygen Species (ROS) and Other Photoprotective Mechanisms -- Acknowledgements. , References -- Chapter 11: Light Harvesting by Long-Wavelength Chlorophyll Forms (Red Forms) in Algae: Focus on their Presence, Distribution and Function -- I. Long Wavelength ("Red") Chlorophyll a Forms: Historical Perspective on Their Discovery and General Overview -- II. Long Wavelength Chlorophyll Forms Associated to Photosystem I -- A. Photosystem I Core Red Forms -- B. Photosystem I External Antenna Red Forms -- C. Nature of Long Wavelength Chlorophyll Forms -- III. Long Wavelength Chlorophyll Forms Associated to Photosystem II -- A. PSII-Associated Long Wavelength Chlorophylls in Algae -- IV. Survey of Cyanobacterial and Algal Species for the Presence of Long-Wavelength Chlorophyll Forms -- A. Physiological and Environmental Consequences of the Presence of Red Forms (or Their Absence) -- V. Effect of Long Wavelength Chlorophyll Forms on the Photochemical Quantum Efficiency -- A. Simulations of the Impact of Red Forms on Excited State Energy Trapping -- 1.. Photosystem I -- 2.. Photosystem II -- VI. Concluding Remarks -- Acknowledgements -- References -- Chapter 12: Diversity in Photoprotection and Energy Balancing in Terrestrial and Aquatic Phototrophs -- I. Introduction -- II. Energy Storage and Regulation in Oxygenic Photosynthesis -- III. The pmf Paradigm for Regulation of the Photosynthetic Light Reactions -- IV. The Need to Coordinate qE and Photosynthetic Control -- V. The Critical Need to Balance the Chloroplast Energy Budget -- VI. Regulation of CEF -- VII. Modulation of pmf Feedback Regulation and Its Impact on Energy Balancing -- VIII. How Diverse Photoprotective Mechanisms Challenge the pmf Paradigm and Open Up New Questions -- IX. Coping with ATP Excess or NADPH Deficit -- A. Energy Balancing by Interactions Between Photosynthetic and Respiratory Machinery -- X. Conclusions and Perspective -- Acknowledgements. , References.
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  • 2
    Publication Date: 2020-02-06
    Description: Contents 670 I. 671 II. 671 III. 676 IV. 678 678 References 678 SUMMARY: Biotic interactions underlie life's diversity and are the lynchpin to understanding its complexity and resilience within an ecological niche. Algal biologists have embraced this paradigm, and studies building on the explosive growth in omics and cell biology methods have facilitated the in-depth analysis of nonmodel organisms and communities from a variety of ecosystems. In turn, these advances have enabled a major revision of our understanding of the origin and evolution of photosynthesis in eukaryotes, bacterial-algal interactions, control of massive algal blooms in the ocean, and the maintenance and degradation of coral reefs. Here, we review some of the most exciting developments in the field of algal biotic interactions and identify challenges for scientists in the coming years. We foresee the development of an algal knowledgebase that integrates ecosystem-wide omics data and the development of molecular tools/resources to perform functional analyses of individuals in isolation and in populations. These assets will allow us to move beyond mechanistic studies of a single species towards understanding the interactions amongst algae and other organisms in both the laboratory and the field.
    Type: Article , PeerReviewed
    Format: text
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The recent discovery of large numbers of phytochrome photoreceptor genes in both photosynthetic and non-photosynthetic prokaryotes has led to efforts to understand their physiological roles in environmental acclimation. One receptor in this class, RcaE, is involved in controlling complementary chromatic adaptation, a process that regulates the transcription of operons encoding light-harvesting proteins in cyanobacteria. Although all previously identified phytochrome responses are maximally sensitive to red and far red light, complementary chromatic adaptation is unique in that it is responsive to green and red light. Here, we present biochemical and genetic evidence demonstrating that RcaE is a photoreceptor and that it requires the cysteine at position 198 to ligate an open chain tetrapyrrole covalently in a manner analogous to chromophore attachment in plant phytochromes. Furthermore, although the wild-type rcaE gene can rescue red and green light photoresponses of an rcaE null mutant, a gene in which the codon for cysteine 198 is converted to an alanine codon rescues the red light but not the green light response. Thus, RcaE is a photoreceptor that is required for both green and red light responsiveness during complementary chromatic adaptation and is the first identified phytochrome class sensor that is involved in sensing and responding to green and red light rather than red and far red light.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Photosynthetic light harvesting in plants is regulated in response to changes in incident light intensity. Absorption of light that exceeds a plant's capacity for fixation of CO2 results in thermal dissipation of excitation energy in the pigment antenna of photosystem II by a poorly ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 38 (2004), S. 119-173 
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: This review focuses on the biosynthesis of pigments in the unicellular alga Chlamydomonas reinhardtii and their physiological and regulatory functions in the context of information gathered from studies of other photosynthetic organisms. C. reinhardtii is serving as an important model organism for studies of photosynthesis and the pigments associated with the photosynthetic apparatus. Despite extensive information pertaining to the biosynthetic pathways critical for making chlorophylls and carotenoids, we are just beginning to understand the control of these pathways, the coordination between pigment and apoprotein synthesis, and the interactions between the activities of these pathways and those for other important cellular metabolites branching from these pathways. Other exciting areas relating to pigment function are also emerging: the role of intermediates of pigment biosynthesis as messengers that coordinate metabolism in the chloroplast with nuclear gene activity, and the identification of photoreceptors and their participation in critical cellular processes including phototaxis, gametogenesis, and the biogenesis of the photosynthetic machinery. These areas of research have become especially attractive for intensive development with the application of potent molecular and genomic tools currently being applied to studies of C. reinhardtii.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 343 (1980), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 30 (1998), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Light-harvesting in cyanobacteria and red algae is a function of the biliproteins, which have covalently bound bilin chromophores. The biliproteins are assembled with linker proteins into the phycobilisome, a large complex that resides on the surface of the photosynthetic membranes. Early steps in the phycobilisome assembly pathway include the folding of biliprotein α- and β-subunits, covalent modification of subunits by bilin attachment and formation of the primary assembly unit, the αβ heterodimer. The potential role of bilins in subunit structure and assembly is examined in this study by site mutagenesis of biliprotein genes. Phycocyanin subunits from Synechocystis sp. 6701 that were unable to bind chromophores at specific sites were generated by changing the codons for bilin-binding cysteines to alanine residues. The altered genes were then expressed in a phycocyanin-minus mutant of the transformable Synechocystis sp. strain 6803. Single and multiple chromophore deletions cause specific and reproducible variations in phycobilisome-associated phycocyanin that do not correlate with transcript levels. Sedimentation equilibrium studies with purified proteins showed that bilin absence reduces the strength of αβ interaction in the heterodimer. These results suggest that phycocyanin instability in bilin-deletion mutants is a consequence of diversion of unassembled α- and β-subunits to a degradation pathway. Attachment of the central bilin, which is common to all biliprotein subunits, may facilitate αβ interaction by completing the final stage of subunit folding and stabilizing the contact domains of binding partners in the heterodimer.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 252 (1996), S. 572-579 
    ISSN: 1617-4623
    Keywords: Key words Bacillariophyta ; Biolistic transformation ; Phleomycin ; Reporter gene ; Selectable marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A nuclear transformation system has been developed for the diatom Phaeodactylum tricornutum using microparticle bombardment to introduce the sh ble gene from Streptoalloteichus hindustanus into cells. The sh ble gene encodes a protein that confers resistance to the antibiotics Zeocin and phleomycin. Chimeric genes containing promoter and terminator sequences from the P. tricornutum fcp genes were used to drive expression of sh ble. Between 10–100 transformants were recovered/108 cells. Transformants were able to grow on at least 500 μg/ml of Zeocin, which is 10 times the amount necessary to kill wild-type cells. Based on Southern hybridizations the sh ble gene was present in 1–3 copies/transformant. Relative levels of correctly processed transcripts were correlated with the abundance of the Sh ble protein (present at 0.1–2.0 μg/mg total protein). The cat reporter gene fused to a fcp promoter could also be introduced by microparticle bombardment and was found to be highly expressed (average of 7.1 U/mg total protein). This work demonstrates that heterologous genes can be readily expressed in P. tricornutum. The development of selectable marker and reporter gene constructs provides the tools necessary for dissecting gene structure and regulation, and introducing novel functions into diatoms.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 35 (1993), S. 235-245 
    ISSN: 1573-5079
    Keywords: allophycocyanin ; anchor protein ; phycoerythrin ; red algae ; rod-core linker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genes encoding the phycobilisome anchor protein (apcE) and rod-core linker (cpcG) are on the plastid genome of the red alga Aglaothamnion neglectum. The apcE gene product is 5′ to and in the same operon as the α and β subunit genes of allophycocyanin. This arrangement is identical to the arrangement observed in many cyanobacteria. The cpcG gene product is 5′ to the operon encoding the α and β subunits of phycoerythrin, but is transcribed from the opposite DNA strand. This gene arrangement is different from that observed in cyanobacteria. The amino acid sequences of the A. neglectum anchor protein and rod-core linker polypeptide, as deduced from the nucleotide sequences of the genes, are approximately 50% identical to analogous polypeptides from cyanobacteria and another eukaryotic alga Cyanophora paradoxa. The conserved nature of these proteins suggests that the structure of the core and the rod-core interface are very similar in phycobilisomes of cyanobacteria and eukaryotic red algae. Environmental factors such as nutrient availability and light intensity can significantly affect the levels of mRNAs encoding the anchor protein and the rod-core linker polypeptide. Most of these changes are similar to the environmentally-controlled changes in the levels of phycobiliprotein transcripts of A. neglectum (Apt and Grossman 1992b). However, unlike the mRNAs encoding other phycobilisome components, the apcE transcript remains high during growth under conditions of nutrient deprivation.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5079
    Keywords: Chlamydomonas reinhardtii ; mutagenesis ; photoinhibition ; Photosystem II ; repair cycle ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The work outlines the isolation of transformant Chlamydomonas reinhardtii cells that appear to be unable to repair Photosystem II from photoinhibitory damage. A physiological and biochemical characterization of three mutants is presented. The results show differential stability for the D1 reaction center protein in the three mutants compared to the wild type and suggest lesions that affect different aspects of the Photosystem II repair mechanism. In the ag16.2 mutant, significantly greater amounts of D1 accumulate in the thylakoid membrane than in the wild type under steady-state growth conditions, and D1 loss is significantly retarded in the presence of the protein biosynthesis inhibitor chloramphenicol. Moreover, aberrant electrophoretic mobility of D1 in the ag16.2 suggests that this protein is modified to an as yet unknown configuration. These results indicate that the biosynthesis and/or degradation of D1 is altered in this strain. A different type of mutation occurred in the kn66.7 and kn27.4 mutants of C. reinhardtii. The stability of D1 declined much faster as a function of light intensity in these mutants than in the wild type. Thereby, the threshold of photoinhibition in these mutants was significantly lower than that in the wild type. It appears that kn66.7 and kn27.4 are similar conditional mutants, with the only difference between them being the amplitude of the chloroplast response to the mutation and the differential sensitivity they display to the level of irradiance.
    Type of Medium: Electronic Resource
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