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  • 1
    In: AMB Express, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2022-12)
    Abstract: This study aims to produce, characterize, and assess the antimicrobial activity and cytotoxicity of polymer blends based on chitosan (CT) and fish collagen (COL) produced by different precipitation methods. Polymer blends were obtained in alkaline (NaOH), saline (NaCl), and alkaline/saline (NaOH/NaCl) solutions with different CT:COL concentration ratios (20:80, 50:50, and 80:20). The polymer blends were characterized by various physicochemical methods and subsequently evaluated in terms of their in vitro antimicrobial and cytotoxicity activity. In this study, the degree of chitosan deacetylation was 82%. The total hydroxyproline and collagen content in the fish matrix was 47.56 mg. g −1 and 394.75 mg. g −1 , respectively. The highest yield was 44% and was obtained for a CT:COL (80:20) blend prepared by precipitation in NaOH. High concentrations of hydroxyproline and collagen in the blends were observed when NaOH precipitation was used. Microbiological analysis revealed that the strains used in this work were sensitive to the biomaterial; this sensitivity was dose-dependent and increased with increasing chitosan concentration in the products. The biocompatibility test showed that the blends did not reduce the viability of fibroblast cells after 48 h of culture. An analysis of the microbiological activity of the all-polymer blends showed a decrease in the values of minimal inhibitory concentration (MIC) and minimal bactericidal concentrations (MBC) for S. aureus and P. aeruginosa . The blends showed biocompatibility with NIH-3T3 murine fibroblast cells and demonstrated their potential for use in biomedical applications such as wound healing, implants, and scaffolds.
    Type of Medium: Online Resource
    ISSN: 2191-0855
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2621432-5
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  • 2
    In: Neurotoxicology and Teratology, Elsevier BV, Vol. 34, No. 5 ( 2012-9), p. 522-529
    Type of Medium: Online Resource
    ISSN: 0892-0362
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2012
    detail.hit.zdb_id: 2008305-1
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  • 3
    In: Regulatory Peptides, Elsevier BV, Vol. 111, No. 1-3 ( 2003-3), p. 61-65
    Type of Medium: Online Resource
    ISSN: 0167-0115
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2003
    detail.hit.zdb_id: 1498712-0
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  • 4
    In: European Journal of Neuroscience, Wiley, Vol. 51, No. 6 ( 2020-03), p. 1377-1387
    Abstract: Microglia cells exert a critical role in brain development, mainly supported by their immune functions, which predicts an impact on the genesis of psychiatric disorders. In fact, microglia stress during gestation is, for instance, associated with chronic anxiety and cognitive deficits accompanied by long‐lasting, region‐ and sex‐specific changes in microglia morphology. We recently reported that the pattern of microglia morphologic plasticity, which is sex‐determined, impacts on anxious‐like behaviour and cognition. We also reported that the pharmacologic blockade of adenosine A 2A receptors (A 2 A R ) is able to reshape microglia morphology, in a sex‐specific manner and with behavioural sequelae. In order to better understand the role of A 2 A R in the sex differentiation of microglia, we now compared their morphology in wild‐type and A 2 A R knockout male and female C57 BL /6 mice in two cardinal brain regions implicated in anxiety‐like behaviour and cognition, the prefrontal cortex ( PFC ) and the dorsal hippocampus ( dHIP ). We report interregional differences between PFC and dHIP in a sex‐specific manner: while males presented more complex microglia in the dHIP , microglia from females had a more complex morphology in the PFC . Surprisingly, the genetic deletion of A 2 A R did not alter these sex differences, but promoted the exclusive remodelling (increase in complexity) in PFC microglia from females. These findings further support the existence of a heterogeneous microglial network, distinct between sexes and brain regions, and help characterizing the role of A 2 A R in the sex‐ and brain region‐specific morphologic differentiation of microglia.
    Type of Medium: Online Resource
    ISSN: 0953-816X , 1460-9568
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2005178-5
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    FapUNIFESP (SciELO) ; 2013
    In:  Revista Brasileira de Medicina do Esporte Vol. 19, No. 1 ( 2013-02), p. 48-51
    In: Revista Brasileira de Medicina do Esporte, FapUNIFESP (SciELO), Vol. 19, No. 1 ( 2013-02), p. 48-51
    Abstract: INTRODUCTION: In rugby, each position has very specific and unique requirements, both anthropometric and physiological. Several studies have documented the significant differences in the anthropometric and physiological characteristics of athletes in the different playing positions. However, despite being common in countries where rugby is more popular, no studies seeking to investigate the anthropometric and physiological characteristics of the Portuguese rugby players have been published yet. OBJECTIVES: To anthropometrically and physiologically characterize Portuguese rugby players, attempting to identify any differences between athletes of different positions and to compare the recorded results with similar studies. METHODS: 46 rugby players from two teams competing in the senior male national championships were assessed. Athletes were grouped according to their positions on the field, as forwards (n = 24) and backs (n = 22). All athletes underwent anthropometric assessment with determination of height, body mass and nine skin folds. Out of these, forty also underwent physical abilities assessment which consisted in determination of speed, acceleration and maximal aerobic capacity. Statistical analysis was performed using the IBM® SPSS® Statistics v.19 and significance level of 5% was considered. RESULTS: Forwards presented average body weight of 96.02 kg (+/-13.44) and 1.80 m (+/-0.06) of height, compared to 76.84 kg (+/-7.28) and 1.73 m (+/-0.06) height of backs. As for the physiological assessment, backs recorded better results. In the 10m test they only took 1.97 s (+/-0.20), while forwards spent 2.10s (+/-0.27). In the speed test, backs also spent 0.36 s less than forwards. Maximal aerobic capacities, weight dependent, recorded by backs (52.33+/-5.41 mlO2/min/kg) were also better than those determined for forwards (46.60+/-5.64mlO2/min/kg). DISCUSSION AND CONCLUSIONS: In the present study forwards were significantly taller, heavier and had higher percentage of body fat than backs. They were also slower and had lower maximal aerobic capacity concerning their body mass. However, they had higher maximal aerobic capacity in absolute value and produced greater momentum. Differences between forwards and backs were consistent with the literature and related to the different roles in the game. Despite its intrinsic limitations, we believe this study is relevant and will promote further investigations about this issue. Similar but larger studies should be conducted in the future so that we can more accurately assess and characterize the Portuguese rugby players.
    Type of Medium: Online Resource
    ISSN: 1517-8692
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2013
    detail.hit.zdb_id: 2122086-4
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  • 6
    Online Resource
    Online Resource
    SAGE Publications ; 1993
    In:  Cephalalgia Vol. 13, No. 2 ( 1993-04), p. 99-101
    In: Cephalalgia, SAGE Publications, Vol. 13, No. 2 ( 1993-04), p. 99-101
    Abstract: Platelet membrane fluidity was measured in migraine patients, with and without aura, using the fluorescent probe TMA-DPH (1–[4–(trimethylammonium) phenyl]-6–hexa-1,3,5–triene). Polarization values for TMA-DPH were significantly higher in the platelet membranes of migraine patients (with or without aura) than in those of healthy subjects. These findings signify decreased membrane fluidity and may explain some modifications in receptors, carriers or enzymes described in platelets of migraine patients.
    Type of Medium: Online Resource
    ISSN: 0333-1024 , 1468-2982
    Language: English
    Publisher: SAGE Publications
    Publication Date: 1993
    detail.hit.zdb_id: 2019999-5
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  • 7
    In: Pharmacology, S. Karger AG, Vol. 91, No. 3-4 ( 2013), p. 153-157
    Abstract: Tramadol is a central-acting analgesic associated with nausea and vomiting. Clinical studies have demonstrated that glucocorticoids have analgesic and antiemetic effects when administered perioperatively. The aim of this study is to test the hypothesis that coadministration of tramadol and dexamethasone decreases both postoperative pain and tramadol requirement by patient-controlled analgesia (PCA). Forty female patients undergoing thyroidectomy under general anesthesia were enrolled in a double-blind randomized controlled study and allocated to receive dexamethasone 4 mg i.v. (dexamethasone group, n = 20) or saline (control group, n = 20). At 0, 1, 2, 4 and 22 h of PCA, tramadol consumption and pain were evaluated. Although pain (numerical rating scale 0–10) was significantly lower in the dexamethasone group compared to the control group (2.9 ± 1.4 vs. 3.8 ± 1.2, p = 0.02) at the beginning of PCA, tramadol demand was not significantly different. Although the results herein show a possible beneficial effect of a preoperative single low dose of dexamethasone on postoperative pain, the hypothesis that this corticosteroid decreases tramadol requirement is not supported.
    Type of Medium: Online Resource
    ISSN: 0031-7012 , 1423-0313
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2013
    detail.hit.zdb_id: 1483550-2
    SSG: 15,3
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  • 8
    Online Resource
    Online Resource
    FapUNIFESP (SciELO) ; 2013
    In:  Revista Brasileira de Medicina do Esporte Vol. 19, No. 1 ( 2013-02), p. 52-55
    In: Revista Brasileira de Medicina do Esporte, FapUNIFESP (SciELO), Vol. 19, No. 1 ( 2013-02), p. 52-55
    Abstract: INTRODUCTION: Since it became professional in 1995, several studies have reported greater differentiation of athletes at all levels for each position. However, despite being common in countries where rugby is more popular, no studies seeking to investigate the anthropometric and physiological characteristics of Portuguese rugby players have been published yet. We sought to evaluate the physiological and anthropometric characteristics of the Portuguese rugby athletes playing in different competitive levels by studying the following variables: age, body mass, stature, body composition, maximal aerobic capacity, acceleration, speed and agility. OBJECTIVES: To anthropometrically and physiologically characterize Portuguese rugby players, attempting to identify any differences between athletes of different competitive levels and to compare the recorded results with similar studies. METHODS: We assessed 46 rugby players from two teams competing in different divisions of the men senior national championships. Out of the 46 athletes evaluated, 24 belonged to a semiprofessional team and 22 to an amateur team. The 46 athletes underwent anthropometric assessment, where stature, body mass and skin folds were determined. Out of these, 40 also underwent physical capabilities assessment which consisted in determining speed and acceleration capability, through 30 and 10 meter- running tests, respectively. Additionally, their maximum aerobic capacity was determined through the Luc Léger field test. Statistical analysis was performed using the IBM® SPSS® Statistics v.19 and a significance level of 5% was considered. RESULTS: It was found that semiprofessionals were on average 3cm taller than backs and presented average body fat percentage of only 15.09% (+ / -6.03) compared to the 22.39% (+/-6.54) of backs. Amateurs were also four years older and presented higher average Body Mass Index than semiprofessionals. Concerning the physical tests, results were similar between groups. Discussion and CONCLUSIONS: In the present study, the expected differences between athletes from different competitive levels were not identified. In fact, regarding body composition and height, we have found an advantage of the semiprofessional athletes. However, the homogeneity observed seems to indicate that Portuguese rugby has not given the qualitative leap yet that professionalism brought up to the countries with greater tradition in this sport.
    Type of Medium: Online Resource
    ISSN: 1517-8692
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2013
    detail.hit.zdb_id: 2122086-4
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  • 9
    Online Resource
    Online Resource
    Wiley ; 2018
    In:  CNS Neuroscience & Therapeutics Vol. 24, No. 2 ( 2018-02), p. 85-97
    In: CNS Neuroscience & Therapeutics, Wiley, Vol. 24, No. 2 ( 2018-02), p. 85-97
    Abstract: Methamphetamine ( METH ) is the primary drug within amphetamine‐type stimulants which are the second most abused group of drugs worldwide. There is no pharmacological treatment addressed specifically to METH addiction, and behavioral therapy is shadowed by poor long‐term recovery and relapse. Therefore, novel approaches to manage METH addiction are an urgent need. This review aims to describe the current state of physical exercise use on methamphetamine addiction management. The following searching terms in PubMed were used: (“physical exercise” OR “exercise”) AND “methamphetamine.” Relevant references from key publications and gray literature were also reviewed to identify additional citations for inclusion. Original investigation regarding physical exercise and methamphetamine addiction (clinical data) or neurobiological mechanisms of physical exercise in animal models of methamphetamine administration (preclinical data) was included. Overall, METH users demonstrated improvements, including better fitness and emotional measures, lower relapse rates, and sustained abstinence when compared to nonexercised individuals. The neurobiological mechanisms of physical exercise in METH users seem to reflect an interplay of several agents, including neurochemicals, oxidative stress, neurogenesis, gliogenesis, and blood‐brain barrier as disclosed by preclinical data. Exercise‐based interventions alone or as a conjoint therapy may be a useful tool for managing METH addiction.
    Type of Medium: Online Resource
    ISSN: 1755-5930 , 1755-5949
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2423467-9
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  • 10
    In: Frontiers in Aging Neuroscience, Frontiers Media SA, Vol. 12 ( 2020-11-23)
    Abstract: Worldwide environmental tragedies of anthropogenic origin causing massive release of metals and other pollutants have been increasing considerably. These pollution outbreaks affect the ecosystems and impact human health. Among those tragedies, recent large-scale environmental disasters in Brazil strongly affected riverside populations, leading to high-risk exposure to methylmercury (MeHg). MeHg is highly neurotoxic to the developing brain. This toxicant causes neural stem cell dysfunction and neurodevelopmental abnormalities. However, less is known about the effects of MeHg in the postnatal neurogenic niche, which harbors neural stem cells and their progeny, in the adult brain. Therefore, taking in consideration the impact of MeHg in human health it is urgent to clarify possible associations between exposure to mercury, accelerated cognitive decline, and neurodegenerative diseases. In this perspectives paper, we discuss the neurotoxic mechanisms of MeHg on postnatal neurogenesis and the putative implications associated with accelerated brain aging and early-onset cognitive decline in populations highly exposed to this environmental neurotoxicant.
    Type of Medium: Online Resource
    ISSN: 1663-4365
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2558898-9
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