In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 5, No. 1 ( 2015-04-08)
Kurzfassung:
We used a photoactive general anesthetic called meta -azi-propofol (AziP m ) to test the selectivity and specificity of alkylphenol anesthetic binding in mammalian brain. Photolabeling of rat brain sections with [ 3 H]AziP m revealed widespread but heterogeneous ligand distribution, with [ 3 H]AziP m preferentially binding to synapse-dense areas compared to areas composed largely of cell bodies or myelin. With [ 3 H]AziP m and propofol, we determined that alkylphenol general anesthetics bind selectively and specifically to multiple synaptic protein targets. In contrast, the alkylphenol anesthetics do not bind to specific sites on abundant phospholipids or cholesterol, although [ 3 H]AziP m shows selectivity for photolabeling phosphatidylethanolamines. Together, our experiments suggest that alkylphenol anesthetic substrates are widespread in number and distribution, similar to those of volatile general anesthetics and that multi-target mechanisms likely underlie their pharmacology.
Materialart:
Online-Ressource
ISSN:
2045-2322
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2015
ZDB Id:
2615211-3
Permalink