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  • 1
    Online Resource
    Online Resource
    Advances in Invertebrates and Fish Telemetry : Proceedings of the Second Conference on Fish Telemetry in Europe, Held in la Rochelle, France, 5-9 April 1997. (1998)
    Dordrecht :Springer Netherlands,
    Details
    Keywords: Fishes-Radio tracking-Congresses. ; Decapoda (Crustacea)-Congresses. ; Electronic books.
    Description / Table of Contents: Proceedings of the Second Conference of Fish Telemetry in Europe held in La Rochelle, April 5-9, 1997.
    Type of Medium: Online Resource
    Pages: 1 online resource (351 pages)
    Edition: 1st ed.
    ISBN: 9789401150903
    Series Statement: Developments in Hydrobiology Series ; v.130
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6497444
    DDC: 597.15072
    Language: English
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    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    A method for the external attachment of acoustic tags on roundfish (1998)
    Springer
    Hydrobiologia 371-372 (1998), S. 113-116 
    Details
    ISSN: 1573-5117
    Keywords: fish ; tagging ; telemetry ; heart rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present paper proposes a new method for the external attachment of an acoustic tag on roundfish. The tag is placed in anterio-ventral position and attached with two wires passed around the pelvic girdle. This tagging method was tested on Atlantic cod and European sea bass using acoustic heart rate transmitters. Preliminary results concerning metabolic demand (oxygen consumption), heart rate and behaviour of free swimming individuals are presented and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017017810099
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Change in vertical distribution of sea bass (Dicentrarchus labrax L.) during a hypoxic episode (1998)
    Springer
    Hydrobiologia 371-372 (1998), S. 207-213 
    Details
    ISSN: 1573-5117
    Keywords: telemetry ; salinity ; oxygen ; diurnal rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The vertical distribution of free-swimming sea bass (Dicentrarchus labrax L.) was investigated in a 26 m3 indoor tank by means of acoustic telemetry. During the experiment the bottom of the tank was made progressively hypoxic. In normoxic conditions, sea bass displayed a clear diurnal rhythm during which they occupied the surface at night and swam deeper in the water column during the day. When a vertical oxygen gradient was established, this behaviour changed and sea bass reduced the amplitude of their daily migration to avoid being exposed to the hypoxic bottom water layer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017030228754
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Heart rate telemetry to study environmental influences on fish metabolic expenditure (1998)
    Springer
    Hydrobiologia 371-372 (1998), S. 215-224 
    Details
    ISSN: 1573-5117
    Keywords: fish ; heart rate ; metabolism ; telemetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Routine metabolic rate (RMR) and heart rate (HR) were measured simultaneously in 15 °C-acclimated and free swimming European sea bass (Dicentrarchus labrax L.) in normoxic and hypoxic conditions. The RMR fell within the boundaries previously reported for that species, i.e. the standard metabolic rate and the $${\text{LOC}}_{{\text{MO}}_2 }$$ -curve which describes the relationship between maximum sustainable oxygen consumption (MO2) and water oxygen concentration (CwO2). Heart rates values distributed below a LOCHR-curve which figures the relationship between the maximum HR and CwO2. A mathematical model describing this relationship is proposed. Using the $${\text{LOC}}_{{\text{MO}}_2 }$$ - and LOCHR-curves, the range of the correlated changes of HR and MO2 is delimited. The influence of CwO2 on these limits is also considered and discussed. Two examples illustrating the limiting effect of low ambient oxygenation level on fish metabolic demand are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017078111916
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of rapid transfer from sea water to fresh water on respiratory variables, blood acid-base status and O2 affinity of haemoglobin in Atlantic salmon (Salmo salar L.) (1990)
    Springer
    Journal of comparative physiology 160 (1990), S. 31-39 
    Details
    ISSN: 1432-136X
    Keywords: Fish ; Salinity ; Respiration ; Acid-base status
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Oxygen consumption, gill ventilation, blood acid-base/ionic status and haemoglobin oxygen affinity were studied in seawater-adapted adult salmon (Salmo salar) during five weeks after transfer into fresh water. Freshwater exposure induced the following changes: Standard oxygen consumption ( $$\dot M_{O_{ 2} } $$ ) and ventilatory flow ( $$\dot V_w $$ ) decreased markedly during the first days after transfer, then decreased more gradually until a new steady-state was achieved at which $$\dot M_{O_{ 2} } $$ and $$\dot V_w $$ were about 80% and 56% of the control values, respectively. The marked increase in oxygen extraction coefficient (Ew O 2) and the marked decrease in the oxygen convection requirement ( $$\dot V_w /\dot M_{O_{{\text{ 2}}} } $$ ) were associated with a reduction in the partial pressure of carbon dioxide in arterial blood (Pa CO 2), in spite of a decrease of both ventilatory flow and water CO2 capacitance. These results suggested that transfer into fresh water induced an increase in branchial diffusive conductance. A biphasic pattern was observed in the time-course of the changes in both plasma ion concentration and acid-base status. During the first 10 days, plasma Na+, K+, and Cl− concentrations fell abruptly, then more gradually. [Cl−] decreased more than [Na+] resulting in a progressive increase in the [Na+]/[Cl−] ratio. During the second phase of acclimation to fresh water plasma Na+, K+, and Cl− concentrations progressively increased. [Cl−] increased more than [Na+], so that [Na+]/[Cl−] ratio decreased. Transfer into fresh water did not significantly change plasma lactate concentration. Upon exposure to fresh water, blood pH increased from 7.94±0.04 to 8.43±0.06 at day 10 and then decreased to 8.08±0.03 at day 34. The increase in blood pH induced by transfer to fresh water initially represented a mixed metabolic/respiratory alkalosis. However, after 15 days Pa CO 2 had returned to pretransfer values and the alkalosis was purely metabolic. The metabolic component of the alkalosis was associated with appropriate changes in the plasma strong ion difference (S.I.D.). Blood alkalosis moved the oxygen dissociation curve to the left, so that P50 was decreased by 30% below the value in seawater for the maximal increase in blood pH. This rise in haemoglobin affinity for O2, associated with a marked increase in blood buffer capacity, are regarded as adaptative processes allowing the salmon to cope with the markedly increased energy expenditure required for upstream migration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00258760
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    Paper (German National Licenses)
    Fulltext
  • 6
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    Experimental conditions for respiration and growth studies of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax (2022)
    PANGAEA
    Details
    Publication Date: 2023-09-28
    Description: Water parameters in the 2 years before spawning of F0 (08.02.2016-06.03.2018) and during larval and juvenile phase of F1: Larval period until 17.05.2018 (48 dph, 900 dd) and 01.06.2018 (63 dph, ~900 dd) for warm and cold life condition respectively, for the juveniles until 28.09.2018 (180 dph, ~4000 dd) and 12.02.2019 (319 dph, ~5100 dd) for warm and cold conditioned fish respectively. Means ± s.e. over all replicate tanks per condition. Temperature (Temp.), pH (free scale), salinity, oxygen and total alkalinity (TA) were measured weekly in F1 and monthly in F0; sea water (SW) measurements were conducted in 2017 and 2018. Water parameters during larval and early juvenile phase of F0: Larval period until (45 dph, 900 dd, 06.12.2013), early juveniles until 1.5 years. Means ± s.e.m. over all measurements per condition (triplicate tanks in larvae, single tanks in juveniles). Temperature (Temp.) and pH (NBS scale) were measured daily. pH (total scale), salinity, phosphate, silicate and total alkalinity (TA) were measured once at the beginning and once at the end of the larval phase and 9 times during juvenile phase; PCO2 was calculated with CO2sys; A–Ambient PCO2, D1000 –ambient + 1000 µatm CO2, L – Larvae, J – Juveniles.
    Keywords: Alkalinity, total; Alkalinity, total, standard error; Calculated; Calculated by CO2sys_xls_program (Lewis and Wallace, 2006); Carbon dioxide (water) partial pressure; DATE/TIME; Generation; juvenile growth; Laboratory experiment; larval growth; Life stage; Measured spectrophotometrically (Dickson et al., 2007) with purified m-cresol purple; metabolic rates; Multiprobe, WTW 340i; Ocean acidification; ocean warming; Oxygen, dissolved; Oxygen, dissolved, standard error; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; pH; pH, standard error; pH meter (WTW 3110) with electrode (WTW Sentix 41); Phosphate; Phosphate, standard error; Salinity; Salinity, standard error; Salinometer (WTW LF325, Xylem Analytics Germany, Weilheim, Germany); SEAL AA3 segmented flow autoanalyzer; Silicate; Silicate, standard error; teleost; Temperature, water; Temperature, water, standard error; Treatment; Type of study; WTW Oxi 340i probe
    Type: Dataset
    Format: text/tab-separated-values, 238 data points
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    DATA PANGAEA
  • 7
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    Experimental conditions, respiration rates of permeabilized heart fibres and biometrical data of European seabass Dicentrarchus labrax (2020)
    PANGAEA
    Details
    Publication Date: 2023-09-30
    Description: The world's oceans are acidifying and warming as a result of increasing atmospheric CO2 concentrations. The thermal tolerance of fish greatly depends on the cardiovascular ability to supply the tissues with oxygen. The highly oxygen-dependent heart mitochondria thus might play a key role in shaping an organism's tolerance to temperature. The present study aimed to investigate the effects of acute and chronic warming on the respiratory capacity of European sea bass (Dicentrarchus labrax L.) heart mitochondria. Broodstock fish were caught in the Gulf of Morbihan, France. Larvae were raised at the aquaculture facility Aquastream (Ploemeur-Lorient, France) and obtained at 2 dph (20 January 2016). European sea bass were reared in the laboratory in six ocean acidification and warming (OAW) conditions: two temperatures (warm and cold life condition) and three PCO2 conditions (control, Δ500 and Δ1000). Conditions were chosen to follow the predictions of the IPCC for the next 130 years: ΔT = 5°C and ΔPCO2 = 500 and 1000 µatm, following RCP 6.0 and RCP 8.5 respectively. The fish were reared under these conditions from 3 dph (days post hatch) until mitochondrial respiration measurements at 3700 to 4100 dd (degree days, 183–199 dph and 234–249 dph in warm and cold life conditioned fish, respectively). During the experimental period, fish of all three PCO2 conditions of the respective temperature were used for mitochondrial respiration measurements on permeabilized heart fibres. Fish were not fed for 2 days prior to the experiments. Two batches of eight fish each were processed per day. Juveniles were randomly caught from their tanks and anesthetized with MS-222. Mass, fork length and body length were directly determined with a precision balance (Mettler, Columbus, OH, USA) and a calliper, to the nearest 0.01 g and 0.01 mm, respectively. Afterwards, fish were killed by a cut through the neck, and the heart was completely dissected from the fish, followed by excavation and permeabilization of the ventricle. Tissue from a whole ventricle was used for respiration measurements in each respiration chamber of the oxygraphs and respiration rates were normalized to ventricle mass. During the permeabilization step, the livers and the carcasses of the fish were weighed to calculate the hepatosomatic index (HSI) and condition factor (K). Mitochondrial respiration of the permeabilized heart fibres was measured using four Oroboros Oxygraph-2K respirometers with DatLab 6 software (Oroboros Instruments, Innsbruck, Austria). Permeabilized fibers have the advantage of resembling the living state as closely as possible, while still allowing control of the supply of substrates and inhibitors to the mitochondria (Saks et al., 1998; Pesta and Gnaiger, 2012). Measurements were conducted at 15 and 20°C for all treatments to determine the effect of acute temperature changes on mitochondrial metabolism in vitro. A standard substrate–uncoupler–inhibitor titration protocol was employed to measure the respiration rates of the different complexes. Residual respiration after antimycin A addition was used to correct all mitochondrial respiration rates.
    Type: Dataset
    Format: application/zip, 2 datasets
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    DATA PANGAEA
  • 8
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    Swimming performance and respiration data of Polar cod (Boreogadus saida) under future ocean conditions (2018)
    PANGAEA
    In:  Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven | Supplement to: Kunz, Kristina Lore; Claireaux, Guy; Pörtner, Hans-Otto; Knust, Rainer; Mark, Felix Christopher (2018): Aerobic capacities and swimming performance of polar cod (Boreogadus saida) under ocean acidification and warming conditions. Journal of Experimental Biology, 221(21), jeb184473, https://doi.org/10.1242/jeb.184473
    Details
    Publication Date: 2023-09-28
    Description: Polar cod (Boreogadus saida) were acclimated for four months to different temperatures (0, 3, 6, 8°C) and PCO2 (390 and 1170 µatm) conditions. Subsequently, B. saida were exercised in a Brett-type swimming tunnel at their respective acclimation conditions the fourth day after feeding. The swimming protocol involved a careful increase in water speed of 1.9 ± 0.3 cm/sec after 11 min. The onset of burst-type swimming behavior indicated the transition from purely aerobic to partly anaerobic swimming speed (Ugait). The critical swimming speed (Ucrit) was reached as soon as the fish touched the grid for at least 30 sec. Ucrit was further adjusted according to Brett (1964). At each velocity step, burst-type swimming events were counted twice for 30 sec after 5 and 10 min. The maximum burst count (max. BC) as well as the number of total bursts throughout the protocol served to classify the individual anaerobic swimming performance of B. saida. Additionally, individual burst events per velocity step are listed ("Burst counts per velocity step_specimen 1-5", https://doi.pangaea.de/10.1594/PANGAEA.889446). Furthermore, a duration of one second per burst was assumed in order to estimate the contribution of anaerobic metabolism (tsbanaerob) during the time between Ugait and Ucrit ("time spent bursting", tsb). Immediately after the termination of the swimming protocol, the aerobic performance of B. saida was recorded in a separate intermittent-flow respiration setup. The initial 5 min of the slope of the first ṀO₂ recording was defined as the maximum metabolic rate (MMR) evoked by exercise. Fish remained in the respiration chambers for approximately 48 h. The standard metabolic rate (SMR) was calculated as the 15%-quantile among ṀO₂ records starting from the second night in the respiration chamber. The difference between MMR and SMR characterized the individual aerobic scope of exercise (AS). The ratio Ucrit/MMR was introduced in order to estimate the energetic efficiency of maximum swimming performance (Emax).
    Type: Dataset
    Format: application/zip, 2 datasets
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    DATA PANGAEA
  • 9
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    Respiration and growth rates of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax in response to ocean acidification and warming (2022)
    PANGAEA
    Details
    Publication Date: 2023-09-28
    Description: Ongoing climate change is leading to warmer and more acidic oceans. The future distribution of fish within the oceans depends on their capacity to adapt to these new environments. Only few studies have examined the effects of ocean acidification (OA) and warming (OW) on the metabolism of long-lived fish over successive generations. We therefore aimed to investigate the effect of OA on larval and juvenile growth and metabolism on two successive generations of European sea bass (Dicentrarchus labrax L.) as well as the effect of OAW on larval and juvenile growth and metabolism of the second generation. European sea bass is a large economically important fish species with a long generation time. F0 larvae were produced at the aquaculture facility Aquastream (Ploemeur-Lorient, France) and obtained at 2 days post-hatch (dph). From 2 dph F0 larvae were reared in the laboratory in two PCO2 conditions (ambient and Δ1000). Larval rearing was performed in a temperature controlled room and water temperatures were fixed to 19°C in F0. In juveniles and adults, water temperatures of F0 sea bass were adjusted to ambient temperature in the Bay of Brest during summer (up to 19°C), but were kept constant at 15 and 12°C for juveniles and adults, respectively, when ambient temperature decreased below these values. F1 embryos were obtained by artificial reproduction of F0 broodstock fish. Fertilized eggs were incubated at 15°C and at the same PCO2 conditions as respective F0. Division of F1 larvae from egg rearing tanks into experimental tanks took place at 2 dph. F1 larvae were reared in four OAW conditions: two temperatures (cold and warm life condition, C and W) and two PCO2 conditions (ambient and Δ1000). Larval rearing was performed in a temperature controlled room and water temperatures were fixed to 15 and 20°C for C and W larvae, respectively. In juveniles, water temperatures of F1 sea bass were adjusted to ambient temperature in the Bay of Brest during summer (up to 19°C), but were kept constant at 15°C when ambient temperature decreased below these values. F1-W was always 5°C warmer than the F1-C treatment. OAW conditions for F0 and F1 rearing were chosen to follow the predictions of the IPCC for the next 130 years: ΔT = 5°C and ΔPCO2 = 1000 µatm, following RCP 8.5. We analysed larval and juvenile growth in F0 and F1. Larval routine metabolic rates (RMR, in F1), juvenile standard metabolic rates (SMR, in F0 and F1) and juvenile critical oxygen concentrations (PO2crit, in F0 and F1) were obtained on individuals via intermittent flow-respirometry. Measurements were conducted at the rearing conditions of the respective larva or juvenile. Fish were fasted for 3h and 48-72h for larvae and juveniles, respectively. After the respirometry trial, larvae were photographed to measure there body length and frozen until measurement of dry mass. Juveniles body length and wet mass was directly determined with calipers and a balance.
    Keywords: juvenile growth; Laboratory experiment; larval growth; metabolic rates; Ocean acidification; ocean warming; teleost
    Type: Dataset
    Format: application/zip, 3 datasets
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    DATA PANGAEA
  • 10
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    Burst performance of Polar cod (Boreogadus saida) under ocean acidification and warming conditions (2018)
    PANGAEA
    In:  Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
    Details
    Publication Date: 2023-09-28
    Keywords: Burst counts, mean; Burst counts per velocity step; Carbon dioxide, partial pressure; Temperature, water; Velocity
    Type: Dataset
    Format: text/tab-separated-values, 831 data points
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    DATA PANGAEA
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