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  • 1
    Publication Date: 2024-02-07
    Description: Pollution of the marine environment is an emerging threat. Nowadays, engineered nanoparticles (〈100 nm) such as zinc, copper and silver are widely used as antimicrobial agents, therefore often present in daily-life products. Consequently, the demand and production of nanoparticles are expected to increase. Here, we specifically focus on silver nanoparticles (AgNP). Once released into the environment, AgNPs pose an obvious ecotoxicological risk, potentially affecting ecosystem structure and functioning. For instance, phytoplankton-derived exudates, rich in acidic polysaccharides and amino acids, can abiotically aggregate into microgels such as transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP). Hence, microgels can bridge dissolved and particulate size fractions and facilitate aggregate formation with organic and mineral particles. Both physical and chemical properties make TEP and CSP attractive nutrient hotspots for heterotrophic bacterioplankton. Bacteria, in turn, utilize extracellular enzymes to access these carbon and nitrogen pools. However, knowledge about the mechanisms by which AgNPs might interact with and affect the biogeochemical cycling of TEP and CSP is still insufficient. Therefore, we conducted a mesocosm experiment in the Eastern Mediterranean Sea and investigated the effects of environmentally relevant concentrations of silver ions (Ag+) and AgNP on the properties of TEP and CSP (i.e., area and abundance) along with enzymatic activity measurements. Our results showed that cyanobacteria were likely the primary source of CSP in the ultra-oligotrophic Mediterranean Sea. Also, CSP contributed more to the microgel pool than TEP, as indicated by a strong relationship between CSP and heterotrophic microbial dynamics. While silver (i.e., Ag+ or AgNP) had overall only marginal effects, both species affected the relationships between cell-specific LAPase activity and CSP and cell-specific APase activity and phosphate levels. Thus, Ag+ and AgNP have the potential to regulate microgel dynamics. However, future studies are needed to derive a robust understanding of the effects of silver pollution on the coupling of microgel formation and degradation and the follow-on effect on biogeochemical cycles.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
    Format: text
    Format: archive
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  • 2
    Publication Date: 2023-12-13
    Keywords: CTD/Rosette; CTD1; CTD12; CTD13; CTD14; CTD15; CTD17; CTD18; CTD19; CTD20; CTD8; CTD-RO; Date/Time of event; DEPTH, water; ECO2; Elevation of event; Event label; Incorporation of radioactive label (Smith and Azam, 1992); Latitude of event; Longitude of event; PaCO2; PaCO2-B1; PaCO2-B2; PaCO2-P0a; PaCO2-P15; PaCO2-P19; PaCO2-P23; PaCO2-P25; PaCO2-P26; PaCO2-P30; PaCO2-P31a; PaCO2-P33; PaCO2-P35; Prokaryotes, production; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; U10/2011; Urania
    Type: Dataset
    Format: text/tab-separated-values, 34 data points
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  • 3
    Publication Date: 2023-12-13
    Keywords: beta-glucosidase activity; CTD/Rosette; CTD1; CTD12; CTD13; CTD14; CTD15; CTD17; CTD18; CTD19; CTD20; CTD8; CTD-RO; Date/Time of event; DEPTH, water; ECO2; Elevation of event; Event label; Latitude of event; Leucine aminopeptidase activity; Longitude of event; PaCO2; PaCO2-B1; PaCO2-B2; PaCO2-P0a; PaCO2-P15; PaCO2-P19; PaCO2-P23; PaCO2-P25; PaCO2-P26; PaCO2-P30; PaCO2-P31a; PaCO2-P33; PaCO2-P35; Phosphatase activity; Spectrofluorometry; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; U10/2011; Urania
    Type: Dataset
    Format: text/tab-separated-values, 102 data points
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  • 4
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    PANGAEA
    In:  Supplement to: Karuza, Ana; Celussi, Mauro; Cibic, Tamara; Del Negro, Paola; De Vittor, Cinzia (2012): Virioplankton and bacterioplankton in a shallow CO2-dominated hydrothermal vent (Panarea Island, Tyrrhenian Sea). Estuarine, Coastal and Shelf Science, 97, 10-18, https://doi.org/10.1016/j.ecss.2011.10.027
    Publication Date: 2024-03-15
    Description: Gas hydrothermal vents are used as a natural analogue for studying the effects of CO2 leakage from hypothetical shallow marine storage sites on benthic and pelagic systems. This study investigated the interrelationships between planktonic prokaryotes and viruses in the Panarea Islands hydrothermal system (southern Tyrrhenian Sea, Italy), especially their abundance, distribution and diversity. No difference in prokaryotic abundance was shown between high-CO2 and control sites. The community structure displayed differences between fumarolic field and the control, and between surface and bottom waters, the latter likely due to the presence of different water masses. Bacterial assemblages were qualitatively dominated by chemo- and photoautotrophic organisms, able to utilise both CO2 and H2S for their metabolic requirements. From significantly lower virioplankton abundance in the proximity of the exhalative area together with particularly low Virus-to-Prokaryotes Ratio, we inferred a reduced impact on prokaryotic abundance and proliferation. Even if the fate of viruses in this particular condition remains still unknown, we consider that lower viral abundance could reflect in enhancing the energy flow to higher trophic levels, thus largely influencing the overall functioning of the system.
    Keywords: Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Calcite saturation state; Calculated; Calculated, see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CO2 vent; Coast and continental shelf; Community composition and diversity; Density; DEPTH, water; Entire community; Epifluorescence microscopy; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Identification; Infrared spectroscopy; LATITUDE; LONGITUDE; Mediterranean Sea; OA-ICC; Ocean Acidification International Coordination Centre; Oxygen saturation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Prokaryotes; Prokaryotes, standard deviation; Salinity; see reference(s); Temperate; Temperature, water; Viral abundance; Virus, standard deviation; Viruses/prokaryotes ratio
    Type: Dataset
    Format: text/tab-separated-values, 4444 data points
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  • 5
    Publication Date: 2024-03-15
    Description: Sampling was carried out between 28th and 31st July 2011 in the Panarea Island area along a 9-station transect. One extra station located north of the vent field was sampled as a reference (i.e., no ventinfluenced). At each station, temperature, salinity, oxygen, and pH vertical profiles were registered using a CTD SBE9 Seabird. Seawater was collected with 10 L Niskin bottles for determining pH, the concentrations of chlorophyll a, macronutrients, dissolved inorganic carbon, particulate organic carbon, and nitrogen concentrations, as well as prokaryotic abundance, the rates of heterotrophic carbon production and extracellular enzymatic activities, and to depict the prokaryotic community structure by 16S rRNA gene amplicon sequencing (at seven stations).
    Keywords: Alkalinity, total; Ammonium; Aragonite saturation state; Bacillariophyceae; beta-glucosidase activity; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, dissolved; Carbon, organic, particulate; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Ciliates, aloricate; CO2 vent; Coast and continental shelf; Coccolithophoridae; Community composition and diversity; CTD, Sea-Bird, SBE 9; CTD/Rosette; CTD1; CTD12; CTD13; CTD14; CTD15; CTD17; CTD18; CTD19; CTD20; CTD8; CTD-RO; Date/Time of event; DEPTH, water; Dinoflagellates; Dinophyceae; ECO2; Element analyser CHNSO, Costech ECS 4010; Elevation of event; Entire community; Epifluorescence microscopy; Event label; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Incorporation of radioactive label (Smith and Azam, 1992); Infrared spectrometry (Shimadzu TOC-V); Latitude of event; Leucine aminopeptidase activity; Longitude of event; Mediterranean Sea; Metazoa; Microscopy; Microzooplankton; Microzooplankton, other; Nanoplankton, heterotrophic; Nanoplankton, phototrophic; Nitrate; Nitrite; Nitrogen, organic, particulate; Nutrient autoanalyzer (Bran and Luebbe, AAIII); OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Oxygen; PaCO2; PaCO2-B1; PaCO2-B2; PaCO2-P0a; PaCO2-P15; PaCO2-P19; PaCO2-P23; PaCO2-P25; PaCO2-P26; PaCO2-P30; PaCO2-P31a; PaCO2-P33; PaCO2-P35; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Phaeopigments; Phosphatase activity; Phosphate; Phytoflagellate; Phytoplankton; Potentiometric titration; Prokaryotes, autotroph; Prokaryotes, heterotroph; Prokaryotes, production; Salinity; Silicon dioxide; Spectrofluorometry; Spectrophotometric; Spectrophotometry; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; Temperate; Temperature, water; Tintinnid; Titration, Winkler; Type of study; U10/2011; Urania; Viral abundance
    Type: Dataset
    Format: text/tab-separated-values, 1452 data points
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  • 6
    Publication Date: 2024-03-15
    Keywords: 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; AIRICA analyzer (Miranda); Alkalinity, total; Alloxanthin; Ammonium; Aragonite saturation state; Bacteriochlorophyll a; Bay of Calvi, Mediterranean Sea; Bicarbonate ion; Calcite saturation state; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carotene; CHEM; Chemistry; Chlorophyll a; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Chlorophyllide a; Colorimetric, GF/F filters, autoanalyzer (Bran Luebbe); CTD; CTD with attached oxygen sensor; DATE/TIME; Day of experiment; Density, sigma, in situ; DEPTH, water, experiment; Diadinoxanthin; Diatoxanthin; Divinyl chlorophyll a; Divinyl chlorophyll b; Element analyser CHN, Costech; Epifluorescence microscopy; Event label; Fluorometer; Fucoxanthin; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); High Performance Liquid Chromatography (HPLC); Irradiance; Lutein; Mediterranean Sea Acidification in a Changing Climate; MedSeA; Neoxanthin; Nitrate; Nitrogen, organic, dissolved; Nitrogen, organic, particulate; Nitrogen, total, particulate; Other event; Oxygen; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Peridinin; pH; Phaeophorbide a; Phaeophytin a; Phosphate; Phosphate, organic, dissolved; Phosphorus, particulate; Prasinoxanthin; Prokaryotes; Salinity; Silicon; Skalar AutoAnalyser; Spectrophotometric method with Liquid Waveguide Capillary Cell (LWCC); Stareso-2012-CTD-outside; Stareso-2012-IWS-outside; Temperature, water; Titration potentiometric, Metrohm; Treatment; UV light photo oxidation then spectrophotometric method with LWCC; Violaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 11785 data points
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  • 7
    Publication Date: 2024-03-15
    Keywords: 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; AIRICA analyzer (Miranda); Alkalinity, total; Alloxanthin; Ammonium; Aragonite saturation state; Bacteria; Bacteria, high DNA fluorescence; Bacteria, low DNA fluorescence; Bacterial production; Bacteriochlorophyll a; Bay of Villefranche, Mediterranean Sea; beta-glucosidase activity; Bicarbonate ion; Calcification/Dissolution; Calcification rate, standard deviation; Calcification rate of calcium carbonate; Calcite saturation state; Calculated; Calculated from linear regression; Calculated using error propagation; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carotene; Chitinase activity; Chlorophyll a; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Chlorophyllide a; Coast and continental shelf; Colorimetric, GF/F filters, autoanalyzer (Bran Luebbe); Community composition and diversity; CTD; CTD with attached oxygen sensor; DATE/TIME; Day of experiment; Density, sigma, in situ; DEPTH, water, experiment; Diadinoxanthin; Diatoxanthin; Divinyl chlorophyll a; Divinyl chlorophyll b; Element analyser CHN, Costech; Entire community; Epifluorescence microscopy; Event label; Exudation as determined by 14C DOC production; Exudation as determined by 14C DOC production, standard deviation; FIA with chemiluminescence detection; Field experiment; Flow cytometry; Fluorometer; Fucoxanthin; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross community production of oxygen; Gross community production of oxygen, standard error; High Performance Liquid Chromatography (HPLC); INCUB; Incubation; Iron, dissolved; Irradiance; Leucine aminopeptidase activity; Lipase activity; Lutein; Mediterranean Sea; Mediterranean Sea Acidification in a Changing Climate; MedSeA; MESO; Mesocosm experiment; Mesocosm or benthocosm; Nanoeukaryotes; Neoxanthin; Net community production of oxygen; Net community production of oxygen, standard error; Nitrate; Nitrogen, organic, dissolved; Nitrogen, total, particulate; OA-ICC; Ocean Acidification International Coordination Centre; Other event; Other metabolic rates; Oxygen; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; Peridinin; pH; Phaeophorbide a; Phaeophytin a; Phosphatase activity; Phosphate; Phosphate, organic, dissolved; Phosphorus, particulate; Picoeukaryotes, autotrophic; Prasinoxanthin; Primary production/Photosynthesis; Primary production of POC as determined by 14C POC production; Primary production of POC as determined by 14C POC production, standard deviation; Prochlorococcus; Prokaryotes; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Silicon; Skalar AutoAnalyser; Spectrophotometric method with Liquid Waveguide Capillary Cell (LWCC); Synechococcus; Temperate; Temperature, water; Titration potentiometric, Metrohm; Transparent exopolymer particles as Gum Xanthan equivalents per volume; Treatment; UV light photo oxidation then spectrophotometric method with LWCC; Validation flag/comment; Villefranche-2013-CTD-perturbation; Villefranche-2013-IWS-perturbation; Violaxanthin; Viral abundance; Virus, high DNA fluorescence; Virus, low DNA fluorescence; Virus, medium DNA fluorescence; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 67487 data points
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  • 8
    Publication Date: 2024-03-15
    Keywords: 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; AIRICA analyzer (Miranda); Alkalinity, total; Alloxanthin; Aragonite saturation state; Bacteria; Bacteria, high DNA fluorescence; Bacteria, low DNA fluorescence; Bacteriochlorophyll a; Bay of Villefranche, Mediterranean Sea; Bicarbonate ion; Calcification rate, standard deviation; Calcification rate of calcium carbonate; Calcite saturation state; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carotene; CHEM; Chemistry; Chlorophyll a; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Chlorophyllide a; Colorimetric, GF/F filters, autoanalyzer (Bran Luebbe); CTD; CTD with attached oxygen sensor; DATE/TIME; Day of experiment; Density, sigma, in situ; DEPTH, water, experiment; Diadinoxanthin; Diatoxanthin; Divinyl chlorophyll a; Element analyser CHN, Costech; Epifluorescence microscopy; Event label; Exudation as determined by 14C DOC production; Exudation as determined by 14C DOC production, standard deviation; FIA with chemiluminescence detection; Flow cytometry; Fluorometer; Fucoxanthin; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); High Performance Liquid Chromatography (HPLC); Iron, dissolved; Irradiance; Lutein; Mediterranean Sea Acidification in a Changing Climate; MedSeA; MESO; Mesocosm experiment; Nanoeukaryotes; Neoxanthin; Nitrate; Nitrogen, organic, dissolved; Nitrogen, total, particulate; Other event; Oxygen; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Peridinin; pH; Phaeophorbide a; Phaeophytin a; Phosphate; Phosphate, organic, dissolved; Phosphorus, particulate; Picoeukaryotes, autotrophic; Prasinoxanthin; Primary production of POC as determined by 14C POC production; Primary production of POC as determined by 14C POC production, standard deviation; Prochlorococcus; Prokaryotes; Salinity; Silicon; Skalar AutoAnalyser; Spectrophotometric method with Liquid Waveguide Capillary Cell (LWCC); Synechococcus; Temperature, water; Titration potentiometric, Metrohm; Transparent exopolymer particles as Gum Xanthan equivalents per volume; Treatment; UV light photo oxidation then spectrophotometric method with LWCC; Validation flag/comment; Villefranche-2013-CTD-outside; Villefranche-2013-IWS-perturbation; Violaxanthin; Viral abundance; Virus, high DNA fluorescence; Virus, low DNA fluorescence; Virus, medium DNA fluorescence; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 6732 data points
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  • 9
    Publication Date: 2024-03-15
    Keywords: 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; AIRICA analyzer (Miranda); Alkalinity, total; Alloxanthin; Ammonium; Aragonite saturation state; Bacteria; Bacteria, high DNA fluorescence; Bacteria, low DNA fluorescence; Bacterial production; Bacterial production, standard error; Bacteriochlorophyll a; Bay of Calvi, Mediterranean Sea; beta-glucosidase activity; Bicarbonate ion; Calcite saturation state; Calculated; Calculated from linear regression; Calculated using error propagation; Calculated using seacarb; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate; Carbon, total, particulate; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carotene; Chitinase activity; Chlorophyll a; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Chlorophyllide a; Coast and continental shelf; Colorimetric, GF/F filters, autoanalyzer (Bran Luebbe); Community composition and diversity; CTD; CTD with attached oxygen sensor; DATE/TIME; Day of experiment; Density, sigma, in situ; DEPTH, water, experiment; Diadinoxanthin; Diatoxanthin; Divinyl chlorophyll a; Divinyl chlorophyll b; Element analyser CHN, Costech; Entire community; Epifluorescence microscopy; Event label; Field experiment; Flow cytometry; Fluorometer; Fucoxanthin; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross community production of oxygen; Gross community production of oxygen, standard error; Gross primary production, 18O; Gross primary production, 18O, standard error; High Performance Liquid Chromatography (HPLC); INCUB; Incubation; Irradiance; Leucine aminopeptidase activity; Lipase activity; Lutein; Mediterranean Sea; Mediterranean Sea Acidification in a Changing Climate; MedSeA; MESO; Mesocosm experiment; Mesocosm or benthocosm; Neoxanthin; Net community production of oxygen; Net community production of oxygen, standard error; Nitrate; Nitrogen, organic, dissolved; Nitrogen, organic, particulate; Nitrogen, total, particulate; Nitrogen fixation rate; OA-ICC; Ocean Acidification International Coordination Centre; Other event; Other metabolic rates; Oxygen; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; Peridinin; pH; Phaeophorbide a; Phaeophytin a; Phosphatase activity; Phosphate; Phosphate, organic, dissolved; Phosphorus, particulate; Picoeukaryotes, autotrophic; Prasinoxanthin; Primary production/Photosynthesis; Prokaryotes; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; see comment; Silicon; Skalar AutoAnalyser; Spectrophotometric method with Liquid Waveguide Capillary Cell (LWCC); Stareso-2012-CTD-perturbation; Stareso-2012-IWS-perturbation; Synechococcus; Temperate; Temperature, water; Titration potentiometric, Metrohm; Treatment; UV light photo oxidation then spectrophotometric method with LWCC; Violaxanthin; Viral abundance; Virus, high DNA fluorescence; Virus, low DNA fluorescence; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 105103 data points
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  • 10
    Publication Date: 2024-02-16
    Description: Three culture experiment on the diatom Thalassiosira rotula were conducted within two 2L-photobioreactors under controlled conditions of light and temperature (20 ± 0.02 °C). During each experiment, diatoms were simultaneously grown in the two bioreactors filled respectively with different media. In one of the two bioreactor microalgae were grown in a natural seawater (NAT) medium, whereas in the second one an artificial seawater medium (ASW) was used. ASW medium was artificially prepared, to reach a modified isotopic composition of DIC using an industrial compressed CO2, and at each experiment a different initial DIC concentration was tested to verify if carbon availability would affect phytoplankton algal metabolism. For NAT medium, a sterile-filtered Adriatic Sea water was used and a natural DIC concentration used. During the experiments, the evolution of T. rotula abundance was investigated, together with the variation of the isotopic composition of dissolved inorganic carbon, phytoplankton organic carbon (as particulate organic carbon) and prokaryotes organic carbon (as the fraction 〈 10 µm of particulate organic carbon). Carbonate system parameters (in situ pHNBS, total alkalinity, DIC and dissolved CO2 concentration), nutrient (dissolved inorganic nitrogen and phosphate) concentration and prokaryotes abundance were also analysed during diatom cultivation.
    Keywords: 13C carbon fixation; 2L-photobioreactors; Alkalinity, total; Autoanalyzer-Colorimeter; Calculated using CO2SYS; Carbon, inorganic, dissolved; Carbon capture and storage; CCS; CO2 leakage; Code; Continuous-flow IRMS Europa 20-20 with ANCA TG trace gas separation module; culture experiment; diatoms; Electrochemical probe; Experiment; Flow-cytometer; IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples; Light microscope; Medium; Nitrogen, inorganic, dissolved; pH; Phosphate; Phytoplankton; Phytoplankton, δ13C, particulate organic carbon; Potentiometric open-cell titration; Prokaryotes; Prokaryotes, δ13C, particulate organic carbon; Thalassiosira rotula; Thalassiosira rotula, standard deviation; Time in hours; δ13C, dissolved inorganic carbon
    Type: Dataset
    Format: text/tab-separated-values, 433 data points
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