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  • 1
    Online-Ressource
    Online-Ressource
    Anaerobic Parasitic Protozoa : Genomics and Molecular Biology. (2010)
    Norfolk :Caister Academic Press,
    Details
    Schlagwort(e): Anaerobic protozoa-Genetics. ; Electronic books.
    Beschreibung / Inhaltsverzeichnis: In this book internationally acclaimed researchers critically review the most important aspects of research on anaerobic parasitic protozoa, providing the first coherent picture of their genomics and molecular biology since the publication of the genomes. Chapters are written from a molecular and genomic perspective and contain speculative models upon which future research efforts can be based. Topics include: the genomes of Entamoeba histolytica, Trichomonas vaginalis, Giardia and other diplomonads; the cytoskeletons of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis; genomic analyses and manipulation of gene expression in Entamoeba histolytica; nuclear and chromosomal structure and replication in Giardia; and the mitochondrion-like organelles of a fourth anaerobe, Blastocystis. Essential reading for all researchers working with these protozoa and related organisms and with eukaryotic model organisms. Recommended text for all parasitology laboratories.
    Materialart: Online-Ressource
    Seiten: 1 online resource (234 pages)
    Ausgabe: 1st ed.
    ISBN: 9781912530861
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6122001
    DDC: 616.96
    Sprache: Englisch
    Anmerkung: Intro -- Contents -- 1 -- 2 -- 3 -- 4 -- 5 -- 6 -- 7 -- 8 -- 9 -- Index.
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  • 2
    Digitale Medien
    Digitale Medien
    Intraspecific Variation and Phylogenetic Relationships in the Genus Entamoeba as Revealed by Riboprinting (1997)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 44 (1997), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Eighty-seven isolates of amebae assigned to the genus Entamoeba have been studied by riboprinting (restriction enzyme polymorphism analysis of polymerase chain reaction amplified small subunit ribosomal RNA genes). Twenty-four distinct patterns were obtained, most of which corresponded to previously described species. In three species (Entamoeba coli, Entamoeba gingivalis and Entamoeba moshkovskii) intraspecific variation was detected that led to the grouping of isolates into ‘ribodemes’ (populations of amebae that share the same riboprint pattern). The riboprint data were used to estimate genetic distances among and within species for the construction of phylogenetic trees based on parsimony and distance analyses. The trees obtained with the two methods are largely congruent. In some cases the estimated distances between species were greater than the upper limit recommended for the fragment comigration method of analysis indicating unusually deep branches within this genus. However, it appears that those species producing cysts with eight nuclei, those producing cysts with one nucleus, and those producing cysts with four nuclei form morphologically based groups that are supported by the riboprint data. The oral parasite Entamoeba gingivalis, which does not encyst, clusters with the third group indicating secondary loss of this ability.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1997.tb05951.x
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  • 3
    Digitale Medien
    Digitale Medien
    Analysis of Genes of Mitochondrial Origin in the Genus Entamoeba (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . The amitochondriate protistan parasite Entamoeba histolytica has lost most mitochondria functions secondarily but has retained a reduced organelle of mitochondrial origin, the mitosome. We here investigate the presence, origins, and expression in other species of Entamoeba of three genes of mitochondrial origin—pyridine nucleotide transhydrogenase and the mitochondrial-type chaperonins cpn60 and hsp70. The genes appear to be present in all species and specifically related, confirming that the E. histolytica mitosomal genes were not acquired recently by lateral transfer from another organism. Detection of expression was not possible in all cases under the culture conditions used, but several genes were induced during recovery from exposure to a heat shock. This includes the transhydrogenase, which to our knowledge has not been shown previously to be a heat-shock protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00119.x
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  • 4
    Digitale Medien
    Digitale Medien
    Riboprinting: A Tool for the Study of Genetic Diversity in Microorganisms (1997)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 44 (1997), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Classical morphology-based methods of taxonomic and phylogenetic analysis are inadequate in many groups of structurally simple eukaryotes. Molecular methods can generate data independently of the complexity of the organisms’ morphology. Riboprinting is one such technique, and involves restriction enzyme analysis of polymerase chain reaction amplified small subunit ribosomal RNA genes. The utility of the method is illustrated with examples from several genera of intestinal and bloodstream parasites. Among the applications of riboprinting are the detection of cryptic genetic variation within species, organism misidentifications and culture mix-ups, independent verification of DNA sequences, and the rapid generation of data useful in phylogenetic analyses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1997.tb05667.x
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  • 5
    Digitale Medien
    Digitale Medien
    Electrophoretic Karyotype and Linkage Groups of the Amoeboflagellate Naegleria gruberi (1990)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 37 (1990), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . We have constructed a molecular karyotype for two strains of Naegleria gruberi using pulsed field gel electrophoresis. Each strain has about 23 chromosomes, considerably more than any previous estimate. These chromosomes range in size from 400 kilobasepairs to over 2,000 kilobasepairs. In Naegleria, construction of the DNA karyotype depends on assessment of the anomalous electrophoretic mobility of the circular ribosomal RNA genes. We have determined the chromosomal locations of an identified unique gene (flagellar calmodulin) and four identified multigene families (α- and β-tubulin, actin, ubiquitin), as well as three differentially expressed genes of unknown functions. The ca. 12 actin genes are dispersed over at least seven chromosomes, whereas the majority of the more than eight α-tubulin genes are confined to a single chromosome. The ubiquitin genes are found on five chromosomes in one strain and seven in the other and the β-tubulin genes are on three or four. Our observations provide a foundation for molecular genetic studies in this organism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1990.tb01164.x
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  • 6
    Digitale Medien
    Digitale Medien
    YI-S, a Casein-free Medium for Axenic Cultivation of Entamoeba histolytica, Related Entamoeba, Giardia intestinalis and Trichomonas vaginalis (1995)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 42 (1995), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Pancreatic digests of casein are major ingredients of media used in the axenic cultivation of lumen-dwelling parasitic protozoa, especially Entamoeba, Giardia, and trichomonads. The digest used almost exclusively in the development of these media, Medo-Peptone (Trypticase® BBL), has not been available since 1981. Moreover, none of dozens of similar type digests tested since then in our laboratory has proved equal to Medo-Peptone, and in the last two years it has become increasingly difficult to obtain new batches which will support even modest growth of Entamoeba histolytica. In response to this problem we have developed a casein-free medium, YI-S, consisting of a nutrient broth, vitamin mixture and serum. We recommend it as a replacement for the casein-dependent medium TYI-S-33, currently the most widely used for axenic culture of Entamoeba histolytica and other lumen-dwellers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1995.tb01579.x
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  • 7
    Digitale Medien
    Digitale Medien
    A Redescription of Entamoeba Histolytica Schaudinn, 1903 (Emended Walker, 1911) Separating It From Entamoeba Dispar Brumpt, 1925 (1993)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 40 (1993), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Explaining the low incidence of invasive disease (10%) in humans infected with Entamoeba histolytica has occupied the attention of generations of both clinical and nonclinical investigators. One possible explanation would be the existence of two morphologically identical species—one an invasive pathogen, the other noninvasive. This was first proposed by Brumpt in 1925, but his explanation was virtually ignored until 1978 when the first of several publications appeared suggesting that E. histolytica did indeed consist of two species. We have reexamined Brumpt's claim in light of recent biochemical, immunological and genetic studies and conclude that the data derived from these investigations provide unequivocal evidence supporting his hypothesis. With this in mind, we redescribe the invasive parasite retaining the name Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911), and set it apart from the noninvasive parasite described by Brumpt, Entamoeba dispar Brumpt, 1925.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1993.tb04926.x
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  • 8
    Digitale Medien
    Digitale Medien
    Circular Ribosomal RNA Genes Are a General Feature of Schizopyrenid Amoebae (1988)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 35 (1988), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: We have recently shown that the ribosomal RNA genes of the amoebo-flagellate Naegleria gruberi Schardinger, 1899, strain NEG-M are carried exclusively on a 14 kilobasepair plasmid. To explore the distribution of this unique gene arrangement, we have examined another strain of N. gruberi and four other species from the order Schizopyrenida. All have this unusual gene arrangement although the size of the plasmid varies widely. Species groups based on morphological criteria do not agree with those resulting from comparison of plasmid restriction enzyme patterns.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1988.tb04352.x
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  • 9
    Digitale Medien
    Digitale Medien
    Axenic Cultivation of Entamoeba dispar Brumpt 1925, Entamoeba insolita Geiman and Wichterman 1937 and Entamoeba ranarum Grassi 1879 (1995)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 42 (1995), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Three species of Entamoeba have been grown in axenic culture for the first time. In two cases, novel methods for adapting the organisms to growth without bacteria were employed. While E. ranarum was axenized by the classic technique of Diamond, from a monoxenic culture with Trypanosoma cruzi as the associate, both E. dispar and E. insolita were first grown in axenic culture medium supplemented with lethally irradiated bacteria. From there, E. insolita was axenized directly, but E. dispar initially required the presence of fixed bacteria. After prolonged culture under this technically axenic but unwieldy culture system, E. dispar was eventually adapted to growth in the absence of added bacteria.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1995.tb05912.x
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  • 10
    Digitale Medien
    Digitale Medien
    Phylogenetic Relationships Among Anuran Trypanosomes as Revealed by Riboprinting (1995)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 42 (1995), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Twenty trypanosome isolates from Anura (frogs and toads) assigned to several species were characterized by riboprinting–restriction enzyme digestion of polymerase chain reaction amplified small subunit ribosomal RNA genes. Restriction site polymorphisms allowed distinction of all the recognized species and no intraspecific variation in riboprint patterns was detected. Phylogenetic reconstruction using parsimony and distance estimates based on restriction fragment comigration showed Trypanosoma chattoni to be only distantly related to the other species, white T. ranarum and T. fallisi appear to be sister taxa despite showing non-overlapping host specificities.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.1995.tb01546.x
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