In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 20 ( 2000-09-26), p. 10838-10843
Abstract:
sqv ( squashed vulva ) genes comprise a
set of eight independent loci in Caenorhabditis
elegans required zygotically for the invagination of vulval
epithelial cells and maternally for normal oocyte formation and embryogenesis. Sequencing of sqv-3 , sqv-7 , and sqv-8 suggested a role for the
encoded proteins in glycolipid or glycoprotein biosynthesis. Using a combination of in vitro analysis of SQV enzymatic
activities, sqv + - mediated
rescue of vertebrate cell lines, and biochemical characterization of sqv mutants, we show that sqv-3 , -7 , and -8 all affect the biosynthesis of
glycosaminoglycans and therefore compromise the function of one specific class of glycoconjugates, proteoglycans. These
findings establish the importance of proteoglycans and their associated glycosaminoglycans in epithelial morphogenesis and
patterning during C. elegans development.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.97.20.10838
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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