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  • 1
    ISSN: 1438-3888
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The reproductibility of tank experiments concerning unicellular marine algal development was analyzed by means of parallel experiments with cultures ofThalassiosira rotula andSkeletonema costatum, using large flexible plastic tanks under semi-natural conditions. The tanks (3–4 m3, 4–5 m deep) were exposed in the German Bight at a station in the outer harbour of helgoland. The water was obtained from the open North Sea in towable tanks; it was filtered (plate filter), enriched with nitrate (20–30 μgat dm−3), phosphate (1.3–2.3 μgat dm−3) and silicate (15–23 μgat dm−3)-nearly natural springtime concentrations in this area-and inoculated with 103–105 cells dm−3. The water was mixed with non-metal stirring equipment. Within 5 days, concentrations of 106–107 cells dm−3 in an exponential growth phase were obtained. In experiments withT. rotula a parallel development was achieved in spite of some contamination by surrounding water. This is the case for nearly all parameters analyzed (nutrient salts, phytoplankton, bacteria, C, N and particulate carbohydrates). The heterotrophic bacteria, which were determined by means of the plate method, reached concentrations of up to 106 (T. rotula) and 105 (S. costatum) CFU cm−3, respectively. They showed a consistent retrograde development at diatom concentrations above a certain level. The crop did not increase again until the diatoms had reached the stationary phase. During exponential growth ofT. rotula (G=8.9–11.7 h) a partially synchronous cell division was observed. There were also rhythms with respect to cell size (pervalvar axes) and chain length (number of cells). For the experiments withS. costatum (G=10–11.4 h) diurnal variations of cell size and chain length occurred. The present results indicate acceptable reproducibility of algal development and related phenomena in enclosed water bodies.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 24 (1974), S. 163-166 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung 1. Für Planktonuntersuchungen in situ wurden einfache Tanks (ca. 4 m3, 5 m Tiefe) konstruiert, mit denen im Vorhafen von Helgoland während der Sommermonate 1972 und 1973 Versuche bis zu 28 Tagen durchgeführt wurden. 2. Lichtdurchlässige Kombinationsfolien aus Polyamid und Polyäthylen erwiesen sich für die an diesem Standort vorkommenden mechanischen Belastungen als geeignet. 3. Für eine kontinuierliche Durchmischung des Tankinhalts wurde ein Rührwerk entwickelt. Alle mit dem Tankinhalt in Berührung kommenden Teile waren aus physiologisch unbedenklichem Kunststoffmaterial gefertigt. 4. Die Tanks eignen sich für Simultanversuche, wie in Versuchen mit Monokulturen gezeigt werden konnte.
    Notes: Abstract For studies of in situ interactions between plankton and ambient water, simple, cylindrical, 5 m deep translucent-foil tanks were constructed using polyamide and polyethylene (layers 30 and 100 μm, respectively, with polyamide layer outside). The tanks were suspended in the water by metal frames fixed to a buoy. The whole construction was anchored in an outer basin of the harbour of Helgoland, N. Sea, and was exposed to tides and waves generated by winds of up to 6 Bft (Beaufort scale). Even under these conditions the construction and its material allowed experiments lasting 2 weeks and longer, during the summer months of 1972 and 1973. The banks' volume, of about 4 m3 each permits experiments with naturally occurring concentrations of nutrients and organisms and large sampling volumes for the determination of trace components. In this way, natural plankton populations in 1972, and monocultures of different diatom species in 1973 were investigated. Under these conditions, different monocultures developed almost identically.
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  • 3
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The physical and chemical properties of the sea surface can be altered considerably by the formation of slicks and the accumulation of particles (including plankton). Investigations of natural slicks near the island of Sylt consisted of analyses of the dissolved and particulate surface-active substances, as well as the identification of plankton and bacteria collected by a surface-film sampler. The analyzed surface material consisted primarily of phytoplankton (Prorocentrum micans in particular), bacteria, detritus and dissolved compounds of fatty acids. These fatty acid compounds alone would not account for the slick. The slick appeared to be caused by the calm weather, the active accumulation of P. micans at the surface, and the increase in bacteria associated with this accumulation. Here a decrease was observed in the dissolved surface-active substances compared with the concentration usually found in normal surface waters, of which the total fatty acid content in a sample was taken as indicator. In some cases a correlation was found between fatty acids and particulate matter in naturally occurring slicks. Particularly large variations were found in the fatty acid patterns in the filtrate and filter residue at a time when no slick was present and no particulate matter had accumulated at the surface.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Due to complex hydrodynamic and biological inhomogeneities, the phytoplankton species succession cannot be satisfactorily observed, apart from the seasonal blooms which occur in temperate waters. Large flexible plastic tanks have proved to be useful for such observations. In 1972, for 28 days, a phytoplankton succession in nutrient-poor water in the outer harbor of Helgoland was observed in a flexible plastic tank (3 m3). During this period, 3 phytoplankton biomass maxima were formed with many significant correlations. In the first 7 days the ammonia concentration decreased from over 6 to 2 μ mol 1-1 for 14 days. The nitrate concentration remained in the range of 3–6 μ mol 1-1 and then fell abruptly to 0–2 μ mol 1-1. The phosphate concentration was about 0.1 μ mol 1-1. Lauderia borealis dominated the first period, and its increase was significantly correlated with the decrease in ammonia. The diatom was succeeded by two dinoflagellates, Dinophysis acuminata and Prorocentrum micans. The last period of the experiment was characterized by a stronger development of Rhizosolenia species. The rapid recovery of the crop in the nutrient-poor water points to intensive remineralization processes. The irregular occurrence of ammonia near the surface was correlated with the appearance of Noctiluca miliaris at this depth. It is expected that repetitions of this type of experiment will permit further explanations of statistical correlations which are not yet clear. As a first step, in order to test hypotheses, a correlation analysis was employed to eliminate the statistically non-significant correlations.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In outdoor tanks enclosed monocultures of Thalassiosira rotula showed partly synchronized cell divisions during exponential growth. The changes in division activity showed a characteristic frequency, which also could be found for cell length (pervalvar axis) and assimilation rate as well as for concentration changes of dissolved free amino acids (DFAA) and ammonia. Excretion of DFAA occurred preferentially during periods of reduced cell division activity, when assimilation was at a higher level. The concentration of ammonia decreased during these periods. The rates per hour of these parameters showed significant multiple correlations. The release of six individual amino acids (ala, β-ala, gly, lys, orn and ser) seemed to be controlled by the physiological status of the diatoms. These amino acids amounted to a portion of about 70–80% of the total DFAA. Uptake and release rates of DFAA nitrogen and ammonia were in the same range with maxima of about 350 ng at nitrogen dm-3 h-1 and litre. These dynamics pointed to strong fluxes of organic bound nitrogen in marine micro-heterotrophs.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 301 (1980), S. 14-16 
    ISSN: 1618-2650
    Keywords: Best. von Phosphor in Wasser ; Spektralphotometrie, AutoAnalyzer, Meerwasser, partikulärer Phosphor, Mo-Komplex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary After filtration of sea water through glass fiber filters, the filters with the residue are digested by heating with sulphuric acid and hydrogen peroxide. The following determination of phosphate is based on the formation of a phosphomolybdenum complex. It has been modified in such a way that the phosphorus content of the samples can be determined with a continuous-flow system with the TechniconAutoAnalyzer direct after digestion. Two methods are described for different sensitivities of 0.1–10μmol P · l−1.
    Notes: Zusammenfassung Der Rückstand bei Filtration von Meerwasser durch Glasfaserfilter wird mit Schwefelsäure und Wasserstoffperoxid aufgeschlossen. Die anschließende Phosphatbestimmung basiert auf der Bildung eines Phosphormolybdän-Komplexes. Sie wurde modifiziert, so daß der Phosphor-Gehalt der Proben sofort nach Aufschluß mit dem Durchflußsystem des Technicon-AutoAnalyzers bestimmt werden kann. Zwei Methoden mit unterschiedlicher Empfindlichkeit (von 0,1–10μmol P · l−1) werden beschrieben.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Aquatic ecology 28 (1994), S. 371-381 
    ISSN: 1573-5125
    Keywords: organic material ; suspended matter ; turbidity zone ; Elbe estuary ; phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the low salinity region of the Elbe estuary in March–April 1992 the turbidity zone was characterized by high loads of suspended matter, 7% of which was organic material (750 μM C) at the surface. Particulate nitrogen, phosphorus and carbohydrates concentrations reached 55 μM N, 10 μM P and more than 15 μM glc. eq., corresponding to 13% of total C, at the surface and increasing threefold near the bottom. In spite of the peaking of particulate organic material levels in the maximum turbidity zone, there were only consistent qualitative changes in total particulate C, N, P, and carbohydrates along the Elbe estuary. Downstream, both the percentage of particulate organic material and the turbidity: organic material ratio decreased, indicating decomposition in the upper estuary and dilution with inorganic suspended matter from the lower estuary. Diatoms, the dominant phytoplankton group, decreased from the upper reaches towards the turbidity zone by 0.3 (surface) and 1.5 mg C l−1 (bottom). This corresponded to 12 and 60% of the decrease in total particulate carbon. Estimated local input of organic carbon by primary production (21 μg Cl−1d−1) was almost compensated by calculated minimum grazing (14 μg C l−1d−1). Considering net primary production and grazing, the dissimilation by zooplankton (5 μg C l−1d−1) and heterotrophic bacterial decomposition (48 μg C l−1d−1), when summed over the estimated flushing time (12 days) represented a loss of suspended organic matter of 0.6 mg Cl−1. Since this was only 20% of the observed decrease in particulate carbon, significant dilution processes must be assumed. Dissolved organic nitrogen decreased from 35 to 10 μM N and dissolvd organic phosphorus from 0.6 to 0.1 μM P towards the sea, mainly due to dilution. The distribution of phosphate, with highest loads in the turbidity maximum of 2.4 μM, suggested an interaction with the accumulated load of particulate material.
    Type of Medium: Electronic Resource
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  • 8
    Publication Date: 2022-05-24
    Description: Three large plastic enclosures (3-4 m3) were anchored in the outer harbour of Helgoland (German Bight) and filled with natural seawater which was filtered free of algae. The enclosed water bodies were enriched with inorganic nutrients and inoculated with the diatom Thalassiosira rotula. During the growth of the algae diurnal changes in concentration of dissolved free amino acids (DFAA) occurred. The periodic concentration changes of individual amino acids with a low carbon to nitrogen ratio showed significant interrelationships with the partly synchronous devisions of the diatoms. From the exponential to the stationary phase the carbon to nitrogen ratio of DFAA shifted to higher values pointing at an adaptation of the organisms to the decreasing inorganic nitrogen source. During the bloom amino acids relatively rich in nitrogen were mainly excreted but by the end of the growth amino acids with a higher carbon content predominated. At phases of high photosynthetic activity the organisms probably reduced the high osmotic pressure by exudation of DFAA.
    Type: Article , NonPeerReviewed
    Format: text
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  • 9
    Publication Date: 2017-02-02
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
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  • 10
    Publication Date: 2017-02-02
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
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