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  • 1
    Keywords: Forschungsbericht ; Chlamydomonas reinhardii ; Gentechnologie ; Stoffwechsel ; Biotechnologie ; Glykolate
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (20 Seiten, 850,37 KB) , Illustrationen, Diagramme
    Language: German
    Note: Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2023-11-15
    Description: Algal pigment concentrations were retrieved from meltponds and leads, during Leg 4 and 5 of the MOSAiC expedition (Multidisciplinary drifting Observatory for the Study of Arctic Climate) in 2020. The summer increase in meltpond area and open lead water in the Arctic associated with increased light availability is of specific significance for biological production within the Arctic system (Smith et al. 2023). Over a period of 3.5 months, 46 samples have been collected and analysed. After extraction in 90 % acetone, samples were analysed using high-performance liquid chromatography (HPLC) on a Waters system. Algal pigments contain a multiple set of information. Firstly, pigment concentrations can show the presence of algal biomass in the various water masses sampled. Secondly, marker pigments can reveal seasonal and temporal dynamics in algal community structure, by discerning specific algal classes like diatoms, cryptophytes, haptophytes and chlorophytes that have specific roles in biogeochemical cycles. Thirdly, certain pigments are indicative of the (photo)-physiological state of micro-algae and fourth, degradation products of the main chlorophyll a pigment further give an indication about senescence and grazing in the various habitats.
    Keywords: 19'-Hexanoyloxy-4-ketofucoxanthin; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; alpha-Carotene; Arctic; Arctic Ocean; beta-Carotene; BUCKET; Bucket water sampling; Chlorophyll a, total; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Comment; community composition; DATE/TIME; DEPTH, water; Diadinoxanthin; Diatoxanthin; Dinoxanthin; Echinenone; Event label; Fucoxanthin; Gear; Gyroxanthin diester; Hand pump; High Performance Liquid Chromatography (HPLC); HP; LATITUDE; leads; LONGITUDE; Lutein; Lycopene; Microalgae; Mosaic; MOSAiC; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Neoxanthin; Peridinin; Pheophorbides; pigments; Polarstern; ponds; Prasinoxanthin; PS122/4; PS122/4_44-118; PS122/4_47-144; PS122/5; PS122/5_59-200; PS122/5_59-202; PS122/5_59-203; PS122/5_59-207; PS122/5_59-208; PS122/5_59-209; PS122/5_59-210; PS122/5_59-211; PS122/5_59-212; PS122/5_59-213; PS122/5_59-291; PS122/5_59-343; PS122/5_59-344; PS122/5_59-391; PS122/5_59-393; PS122/5_60-130; PS122/5_60-202; PS122/5_60-280; PS122/5_60-64; PS122/5_61-205; PS122/5_61-273; PS122/5_61-278; PS122/5_62-117; PS122/5_62-33; PS122/5_62-40; PS122/5_62-92; PS122/5_63-82; PS122/5_63-84; Sample code/label; Violaxanthin; Water sample; WS; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 1242 data points
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2024-03-01
    Description: First-year sea-ice thickness, draft, salinity, temperature, and density were measured during near-weekly surveys at the main first-year ice coring site (MCS-FYI) during the MOSAiC expedition (legs 1 to 4). The ice cores were extracted either with a 9-cm (Mark II) or 7.25-cm (Mark III) internal diameter ice corers (Kovacs Enterprise, US). This data set includes data from 23 coring site visits and were performed from 28 October 2019 to 29 July 2020 at coring locations within 130 m to each other in the MOSAiC Central Observatory. During each coring event, ice temperature was measured in situ from a separate temperature core, using Testo 720 thermometers in drill holes with a length of half-core-diameter at 5-cm vertical resolution. Ice bulk practical salinity was measured from melted core sections at 5-cm resolution using a YSI 30 conductivity meter. Ice density was measured using the hydrostatic weighing method (Pustogvar and Kulyakhtin, 2016) from a density core in the freezer laboratory onboard Polarstern at the temperature of –15°C. Relative volumes of brine and gas were estimated from ice salinity, temperature and density using Cox and Weeks (1983) for cold ice and Leppäranta and Manninen (1988) for ice warmer than –2°C. The data contains the event label (1), time (2), and global coordinates (3,4) of each coring measurement and sample IDs (13, 15). Each salinity core has its manually measured ice thickness (5), ice draft (6), core length (7), and mean snow height (22). Each core section has the total length of its top (8) and bottom (9) measured in situ, as well estimated depth of section top (10), bottom (11), and middle (12). The depth estimates assume that the total length of all core sections is equal to the measured ice thickness. Each core section has the value of its practical salinity (14), isotopic values (16, 17, 18) (Meyer et al., 2000), as well as sea ice temperature (19) and ice density (20) interpolated to the depth of salinity measurements. The global coordinates of coring sites were measured directly. When it was not possible, coordinates of the nearby temperature buoy 2019T66 were used. Ice mass balance buoy 2019T66 installation is described in doi:10.1594/PANGAEA.938134. Brine volume (21) fraction estimates are presented only for fraction values from 0 to 30%. Each core section also has comments (23) describing if the sample is from a false bottom, from rafted ice or has any other special characteristics. Macronutrients from the salinity core, and more isotope data will be published in a subsequent version of this data set.
    Keywords: Arctic; Arctic Ocean; Arctic Research Icebreaker Consortium: A strategy for meeting the needs for marine-based research in the Arctic; ARICE; Calculated; Comment; Core length; cores; DATE/TIME; density; Density, ice; Depth, adjusted; Depth, adjusted bottom; Depth, adjusted top; Depth, ice/snow, bottom/maximum; Depth, ice/snow, top/minimum; Deuterium excess; Ecological monitoring; Event label; HAVOC; Hydrostatic weighing; IC; Ice corer; ICEGAUGE; Ice thickness gauge; Isotopic liquid water analyzer; LATITUDE; LONGITUDE; MOSAiC; MOSAiC_ECO; MOSAiC_ICE; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Physical properties; Polarstern; PS122/1; PS122/1_10-19; PS122/1_5-3; PS122/1_6-34; PS122/1_7-6; PS122/1_7-97; PS122/1_8-2; PS122/1_9-6; PS122/1_9-93; PS122/2; PS122/2_17-3; PS122/2_19-7; PS122/2_21-13; PS122/2_23-3; PS122/2_24-8; PS122/3; PS122/3_32-63; PS122/3_35-11; PS122/3_36-21; PS122/3_38-24; PS122/3_39-7; PS122/4; PS122/4_44-134; PS122/4_46-18; PS122/4_47-16; PS122/4_48-23; PS122/4_49-34; Ridges - Safe HAVens for ice-associated Flora and Fauna in a Seasonally ice-covered Arctic OCean; Salinity; Salinometer, inductive; Sample ID; Sea ice; Sea ice draft; Sea ice salinity; Sea ice thickness; Snow height; Tape measure; Temperature; Temperature, ice/snow; Thermometer; time-series; Volume, brine; δ18O, water; δ Deuterium, water
    Type: Dataset
    Format: text/tab-separated-values, 7847 data points
    Location Call Number Limitation Availability
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  • 4
    Publication Date: 2024-03-02
    Description: Algal pigment concentrations were retrieved from ocean samples, during the whole MOSAiC expedition (Multidisciplinary drifting Observatory for the Study of Arctic Climate) in 2019 to 2020. During MOSAiC, RV Polarstern anchored into an ice floe to gain new insights into Arctic climate over a full annual cycle. Ocean data were collected starting with the onset of the study, at 85 degrees north and 137 degrees east, following the drift towards the Fram Strait, and returning to the North Pole in the last leg of the expedition. Ocean samples were collected with a CTD, either from the ship, or from the ice floe in Ocean City (Rabe et al. 2022). Altogether 216 samples have been collected and analysed. After extraction in 90 % acetone, samples were analysed using high-performance liquid chromatography (HPLC) on a Waters system. Algal pigments contain a multiple set of information. Firstly, pigment concentrations can show the presence of algal biomass in the various water masses sampled. Secondly, marker pigments can reveal seasonal and temporal dynamics in algal community structure, by discerning specific algal classes like diatoms, cryptophytes, haptophytes and chlorophytes that have specific roles in biogeochemical cycles. Thirdly, certain pigments are indicative of the (photo)-physiological state of micro-algae and fourth, degradation products of the main chlorophyll a pigment further give an indication about senescence and grazing in the various habitats.
    Keywords: 19'-Hexanoyloxy-4-ketofucoxanthin; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; alpha-Carotene; Arctic Ocean; beta-Carotene; Chlorophyll a, total; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Comment; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; Diadinoxanthin; Diatoxanthin; Dinoxanthin; Echinenone; Event label; Fucoxanthin; Gear; Gyroxanthin diester; High Performance Liquid Chromatography (HPLC); LATITUDE; LONGITUDE; Lutein; Lycopene; MOSAiC; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Neoxanthin; Peridinin; Pheophorbides; Polarstern; Prasinoxanthin; PS122/1; PS122/1_10-44; PS122/1_6-58; PS122/1_7-49; PS122/1_8-46; PS122/1_9-50; PS122/2; PS122/2_17-41; PS122/2_18-34; PS122/2_19-56; PS122/2_20-46; PS122/2_21-65; PS122/2_22-47; PS122/2_23-63; PS122/2_25-54; PS122/3; PS122/3_30-40; PS122/3_30-53; PS122/3_31-39; PS122/3_31-59; PS122/3_32-75; PS122/3_33-69; PS122/3_34-77; PS122/3_34-97; PS122/3_35-63; PS122/3_36-81; PS122/3_37-45; PS122/3_38-54; PS122/3_39-51; PS122/3_40-36; PS122/4; PS122/4_44-184; PS122/4_44-67; PS122/4_45-31; PS122/4_46-60; PS122/4_47-60; PS122/4_48-62; PS122/4_49-10; PS122/4_49-25; PS122/4_50-21; PS122/5; PS122/5_59-274; PS122/5_59-306; PS122/5_59-62; PS122/5_59-72; PS122/5_60-69; PS122/5_61-161; PS122/5_63-53; PUMP; Sample code/label; Violaxanthin; Water pump; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 5633 data points
    Location Call Number Limitation Availability
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  • 5
    Publication Date: 2024-04-24
    Description: Second-year sea-ice thickness, draft, salinity, temperature, and density were measured during near-weekly surveys at the main second-year ice coring site (MCS-SYI) during the MOSAiC expedition (legs 1 to 3) and new second-year ice coring site leg 4, since the earlier site was not accessible any longer. The ice cores were extracted either with a 9-cm (Mark II) or 7.25-cm (Mark III) internal diameter ice corers (Kovacs Enterprise, US). This data set includes data from 18 coring site visits and were performed from 28 October 2019 to 20 July 2020 at coring locations within 50 m to each other in the MOSAiC Central Observatory. During each coring event, ice temperature was measured in situ from a separate temperature core, using Testo 720 thermometers in drill holes with a length of half-core-diameter at 5-cm vertical resolution. Ice bulk practical salinity was measured from melted core sections at 5-cm resolution using a YSI 30 conductivity meter. Ice density was measured using the hydrostatic weighing method (Pustogvar and Kulyakhtin, 2016) from a density core in the freezer laboratory onboard Polarstern at the temperature of –15°C. Relative volumes of brine and gas were estimated from ice salinity, temperature and density using Cox and Weeks (1983) for cold ice and Leppäranta and Manninen (1988) for ice warmer than –2°C. The data contains the event label (1), time (2), and global coordinates (3,4) of each coring measurement and sample IDs (13, 15). Each salinity core has its manually measured ice thickness (5), ice draft (6), core length (7), and mean snow height (22). Each core section has the total length of its top (8) and bottom (9) measured in situ, as well estimated depth of section top (10), bottom (11), and middle (12). The depth estimates assume that the total length of all core sections is equal to the measured ice thickness. Each core section has the value of its practical salinity (14), isotopic values (16, 17, 18) (Meyer et al., 2000), as well as sea ice temperature (19) and ice density (20) interpolated to the depth of salinity measurements. The global coordinates of coring sites were measured directly. When it was not possible, coordinates of the nearby temperature buoy 2019T62 (legs 1-3) or 2019T61 (leg 4) were used. Ice mass balance buoy 2019T62 installation is described in doi:10.1594/PANGAEA.940231, ice mass balance buoy 2020T61 installation is described in doi: 10.1594/PANGAEA.926580. Brine volume (21) fraction estimates are presented only for fraction values from 0 to 30%. Each core section also has comments (23) describing if the sample is from a new coring site or has any other special characteristics. Macronutrients from the salinity core will be published in a subsequent version of this data set.
    Keywords: Arctic; Arctic Ocean; Arctic Research Icebreaker Consortium: A strategy for meeting the needs for marine-based research in the Arctic; ARICE; Calculated; Comment; Core length; cores; DATE/TIME; density; Density, ice; Depth, adjusted; Depth, adjusted bottom; Depth, adjusted top; Depth, ice/snow, bottom/maximum; Depth, ice/snow, top/minimum; Deuterium excess; Ecological monitoring; Event label; HAVOC; Hydrostatic weighing; IC; Ice corer; ICEGAUGE; Ice thickness gauge; Isotopic liquid water analyzer; LATITUDE; LONGITUDE; MOSAiC; MOSAiC_ECO; MOSAiC_ICE; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Physical properties; Polarstern; PS122/1; PS122/1_10-16; PS122/1_5-78; PS122/1_6-36; PS122/1_7-53; PS122/1_7-9; PS122/1_9-11; PS122/2; PS122/2_20-5; PS122/2_22-7; PS122/2_25-15; PS122/3; PS122/3_33-18; PS122/3_35-4; PS122/3_36-4; PS122/3_38-16; PS122/3_39-18; PS122/4; PS122/4_45-29; PS122/4_46-20; PS122/4_47-18; PS122/4_48-25; Ridges - Safe HAVens for ice-associated Flora and Fauna in a Seasonally ice-covered Arctic OCean; Salinity; Temperature; Salinometer, inductive; Sample ID; Sea ice; Sea ice draft; Sea ice salinity; Sea ice thickness; Snow height; Tape measure; Temperature, ice/snow; Thermometer; time-series; Volume, brine; δ18O, water; δ Deuterium, water
    Type: Dataset
    Format: text/tab-separated-values, 9395 data points
    Location Call Number Limitation Availability
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  • 6
    Publication Date: 2024-04-26
    Description: Algal pigment concentrations were retrieved from samples from first-year (FYI) ice, during the whole MOSAiC expedition (Multidisciplinary drifting Observatory for the Study of Arctic Climate) in 2019 to 2020. During MOSAiC, RV Polarstern anchored into an ice floe to gain new insights into Arctic climate over a full annual cycle. Sea ice data were collected starting with the onset of the study, at 85 degrees north and 137 degrees east, following the drift towards the Fram Strait, and returning to the North Pole in the last leg of the expedition. Ice cores were collected at the various coring sites together with teams ICE and BGC (Nicolaus et al. 2022), to study the development of pigment patterns over time, on 3 specific ice-locations. Altogether, 292 samples have been collected and analysed. Ice cores were sliced in sections of 5-10 cm before analyses. Each of the sections was melted at room temperature after additions of filtered ambient seawater, under dark conditions. After extraction in 90 % acetone, samples were analysed using high-performance liquid chromatography (HPLC) on a Waters system (AWI). Algal pigments contain a multiple set of information. Firstly, pigment concentrations can show the presence of algal biomass in the various domains sampled. Secondly, marker pigments can reveal seasonal and temporal dynamics in algal community structure, by discerning specific algal classes like diatoms, cryptophytes, haptophytes and chlorophytes that have specific roles in biogeochemical cycles. Thirdly, certain pigments are indicative of the (photo)-physiological state of micro-algae and fourth, degradation products of the main chlorophyll a pigment further give an indication about senescence and grazing in the various habitats.
    Keywords: 19'-Hexanoyloxy-4-ketofucoxanthin; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; alpha-Carotene; Arctic Ocean; Astaxanthin; beta-Carotene; Chlorophyll a, total; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; DATE/TIME; Depth, description; DEPTH, ice/snow; Diadinoxanthin; Diatoxanthin; Dinoxanthin; Echinenone; Event label; Fucoxanthin; Gyroxanthin diester; High Performance Liquid Chromatography (HPLC); IC; Ice corer; LATITUDE; LONGITUDE; Lutein; Lycopene; MOSAiC; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Neoxanthin; Peridinin; Pheophorbides; Pheophytin a; Polarstern; Prasinoxanthin; PS122/1; PS122/1_10-19; PS122/1_5-3; PS122/1_6-34; PS122/1_7-6; PS122/1_8-2; PS122/1_9-6; PS122/2; PS122/2_17-3; PS122/2_19-7; PS122/2_21-13; PS122/2_23-3; PS122/2_24-8; PS122/3; PS122/3_35-11; PS122/3_36-21; PS122/3_38-24; PS122/3_39-7; PS122/4; PS122/4_44-134; PS122/4_44-42; PS122/4_46-18; PS122/4_47-16; PS122/4_48-23; PS122/4_49-34; PS122/5; PS122/5_60-3; PS122/5_61-6; Sample code/label; Violaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 8175 data points
    Location Call Number Limitation Availability
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  • 7
    Publication Date: 2024-04-26
    Description: Algal pigment concentrations were retrieved from samples from second-year (SYI) ice, during the whole MOSAiC expedition (Multidisciplinary drifting Observatory for the Study of Arctic Climate) in 2019 to 2020. During MOSAiC, RV Polarstern anchored into an ice floe to gain new insights into Arctic climate over a full annual cycle. Sea ice data were collected starting with the onset of the study, at 85 degrees north and 137 degrees east, following the drift towards the Fram Strait, and returning into the marginal ice zone during the 4th leg of the expedition. Ice cores were collected at the various coring sites together with teams ICE and BGC (Nicolaus et al. 2022), to study the development of pigment patterns over time, on 3 specific ice-locations. Altogether, 277 samples have been collected and analysed. Ice cores were sliced in sections of 5-10 cm before analyses. Each of the sections was melted at room temperature after additions of filtered ambient seawater, under dark conditions. After extraction in 90 % acetone, samples were analysed using high-performance liquid chromatography (HPLC) on a Waters system (AWI). Algal pigments contain a multiple set of information. Firstly, pigment concentrations can show the presence of algal biomass in the various domains sampled. Secondly, marker pigments can reveal seasonal and temporal dynamics in algal community structure, by discerning specific algal classes like diatoms, cryptophytes, haptophytes and chlorophytes that have specific roles in biogeochemical cycles. Thirdly, certain pigments are indicative of the (photo)-physiological state of micro-algae and fourth, degradation products of the main chlorophyll a pigment further give an indication about senescence and grazing in the various habitats.
    Keywords: 19'-Hexanoyloxy-4-ketofucoxanthin; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; alpha-Carotene; Arctic Ocean; Astaxanthin; beta-Carotene; Chlorophyll a, total; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Comment; DATE/TIME; Depth, description; DEPTH, ice/snow; Diadinoxanthin; Diatoxanthin; Dinoxanthin; Echinenone; Event label; Fucoxanthin; Gyroxanthin diester; High Performance Liquid Chromatography (HPLC); IC; Ice corer; LATITUDE; LONGITUDE; Lutein; Lycopene; MOSAiC; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Neoxanthin; Peridinin; Pheophorbides; Pheophytin a; Polarstern; Prasinoxanthin; PS122/1; PS122/1_10-16; PS122/1_5-78; PS122/1_6-36; PS122/1_7-9; PS122/1_9-11; PS122/2; PS122/2_20-5; PS122/2_22-7; PS122/2_24-34; PS122/2_25-15; PS122/3; PS122/3_33-18; PS122/3_36-4; PS122/3_38-16; PS122/3_39-18; PS122/4; PS122/4_45-29; PS122/4_46-20; PS122/4_47-18; PS122/4_48-25; Sample code/label; Violaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 7755 data points
    Location Call Number Limitation Availability
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  • 8
    Publication Date: 2024-04-26
    Description: Algal pigment concentrations were retrieved from samples from first-year (FYI) ice, during the whole MOSAiC expedition (Multidisciplinary drifting Observatory for the Study of Arctic Climate) in 2019 to 2020. During MOSAiC, RV Polarstern anchored into an ice floe to gain new insights into Arctic climate over a full annual cycle. Sea ice data were collected starting with the onset of the study, at 85 degrees north and 137 degrees east, following the drift towards the Fram Strait, and returning to the North Pole in the last leg of the expedition. Ice cores were collected at the various coring sites together with teams ICE and BGC (Nicolaus et al. 2022), to study the development of pigment patterns over time, on 3 specific ice-locations. Altogether, 292 samples have been collected and analysed. Ice cores were sliced in sections of 5-10 cm before analyses. Each of the sections was melted at room temperature after additions of filtered ambient seawater, under dark conditions. After extraction in 90 % acetone, samples were analysed using high-performance liquid chromatography (HPLC) on a Waters system (AWI). Algal pigments contain a multiple set of information. Firstly, pigment concentrations can show the presence of algal biomass in the various domains sampled. Secondly, marker pigments can reveal seasonal and temporal dynamics in algal community structure, by discerning specific algal classes like diatoms, cryptophytes, haptophytes and chlorophytes that have specific roles in biogeochemical cycles. Thirdly, certain pigments are indicative of the (photo)-physiological state of micro-algae and fourth, degradation products of the main chlorophyll a pigment further give an indication about senescence and grazing in the various habitats.
    Keywords: 19'-Hexanoyloxy-4-ketofucoxanthin; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; alpha-Carotene; Arctic Ocean; Astaxanthin; beta-Carotene; Chlorophyll a, total; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; DATE/TIME; Depth, description; DEPTH, ice/snow; Diadinoxanthin; Diatoxanthin; Dinoxanthin; Echinenone; Event label; Fucoxanthin; Gyroxanthin diester; High Performance Liquid Chromatography (HPLC); IC; Ice corer; LATITUDE; LONGITUDE; Lutein; Lycopene; MOSAiC; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Neoxanthin; Peridinin; Pheophorbides; Pheophytin a; Polarstern; Prasinoxanthin; PS122/1; PS122/1_10-16; PS122/1_5-3; PS122/1_6-34; PS122/1_7-6; PS122/1_8-2; PS122/1_9-6; PS122/2; PS122/2_17-3; PS122/2_19-7; PS122/2_21-13; PS122/2_23-3; PS122/2_24-8; PS122/3; PS122/3_35-11; PS122/3_36-21; PS122/3_38-24; PS122/3_39-7; PS122/4; PS122/4_44-134; PS122/4_44-42; PS122/4_46-18; PS122/4_47-16; PS122/4_48-23; PS122/4_49-34; PS122/5; PS122/5_60-3; PS122/5_61-6; Sample code/label; Violaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 8035 data points
    Location Call Number Limitation Availability
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  • 9
    Publication Date: 2024-04-26
    Description: Algal pigment concentrations were retrieved from samples from second-year (SYI) ice, during the whole MOSAiC expedition (Multidisciplinary drifting Observatory for the Study of Arctic Climate) in 2019 to 2020. During MOSAiC, RV Polarstern anchored into an ice floe to gain new insights into Arctic climate over a full annual cycle. Sea ice data were collected starting with the onset of the study, at 85 degrees north and 137 degrees east, following the drift towards the Fram Strait, and returning into the marginal ice zone during the 4th leg of the expedition. Ice cores were collected at the various coring sites together with teams ICE and BGC (Nicolaus et al. 2022), to study the development of pigment patterns over time, on 3 specific ice-locations. Altogether, 277 samples have been collected and analysed. Ice cores were sliced in sections of 5-10 cm before analyses. Each of the sections was melted at room temperature after additions of filtered ambient seawater, under dark conditions. After extraction in 90 % acetone, samples were analysed using high-performance liquid chromatography (HPLC) on a Waters system (AWI). Algal pigments contain a multiple set of information. Firstly, pigment concentrations can show the presence of algal biomass in the various domains sampled. Secondly, marker pigments can reveal seasonal and temporal dynamics in algal community structure, by discerning specific algal classes like diatoms, cryptophytes, haptophytes and chlorophytes that have specific roles in biogeochemical cycles. Thirdly, certain pigments are indicative of the (photo)-physiological state of micro-algae and fourth, degradation products of the main chlorophyll a pigment further give an indication about senescence and grazing in the various habitats.
    Keywords: 19'-Hexanoyloxy-4-ketofucoxanthin; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; alpha-Carotene; Arctic Ocean; Astaxanthin; beta-Carotene; Chlorophyll a, total; Chlorophyll b; Chlorophyll c1+c2; Chlorophyll c3; Comment; DATE/TIME; Depth, description; DEPTH, ice/snow; Diadinoxanthin; Diatoxanthin; Dinoxanthin; Echinenone; Event label; Fucoxanthin; Gyroxanthin diester; High Performance Liquid Chromatography (HPLC); IC; Ice corer; LATITUDE; LONGITUDE; Lutein; Lycopene; MOSAiC; MOSAiC20192020; Multidisciplinary drifting Observatory for the Study of Arctic Climate; Neoxanthin; Peridinin; Pheophorbides; Pheophytin a; Polarstern; Prasinoxanthin; PS122/1; PS122/1_10-19; PS122/1_5-78; PS122/1_6-36; PS122/1_7-9; PS122/1_9-11; PS122/2; PS122/2_20-5; PS122/2_22-7; PS122/2_24-34; PS122/2_25-15; PS122/3; PS122/3_33-18; PS122/3_36-4; PS122/3_38-16; PS122/3_39-18; PS122/4; PS122/4_45-29; PS122/4_46-20; PS122/4_47-18; PS122/4_48-25; Sample code/label; Violaxanthin; Zeaxanthin
    Type: Dataset
    Format: text/tab-separated-values, 7811 data points
    Location Call Number Limitation Availability
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  • 10
    Publication Date: 2021-01-25
    Description: In the Southern Ocean (SO), iron (Fe) limitation strongly inhibits phytoplankton growth and generally decreases their primary productivity. Diatoms are a key component in the carbon (C) cycle, by taking up large amounts of anthropogenic CO2 through the biological carbon pump. In this study, we investigated the effects of Fe availability (no Fe and 4 nM FeCl3 addition) on the physiology of Chaetoceros cf. simplex, an ecologically relevant SO diatom. Our results are the first combining oxygen evolution and uptake rates with particulate organic carbon (POC) build up, pigments, photophysiological parameters and intracellular trace metal (TM) quotas in an Fe-deficient Antarctic diatom. Decreases in both oxygen evolution (through photosynthesis, P) and uptake (respiration, R) coincided with a lowered growth rate of Fe-deficient cells. In addition, cells displayed reduced electron transport rates (ETR) and chlorophyll a (Chla) content, resulting in reduced cellular POC formation. Interestingly, no differences were observed in non-photochemical quenching (NPQ) or in the ratio of gross photosynthesis to respiration (GP:R). Furthermore, TM quotas were measured, which represent an important and rarely quantified parameter in previous studies. Cellular quotas of manganese, zinc, cobalt and copper remained unchanged while Fe quotas of Fe-deficient cells were reduced by 60% compared with High Fe cells. Based on our data, Fe-deficient Chaetoceros cf. simplex cells were able to efficiently acclimate to low Fe conditions, reducing their intracellular Fe concentrations, the number of functional reaction centers of photosystem II (RCII) and photosynthetic rates, thus avoiding light absorption rather than dissipating the energy through NPQ. Our results demonstrate how Chaetoceros cf. simplex can adapt their physiology to lowered assimilatory metabolism by decreasing respiratory losses.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
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