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  • 1
    Electronic Resource
    Electronic Resource
    Methanotrophic TCE Biodegradation in a Multi-Stage Bioreactor (1995)
    s.l. : American Chemical Society
    Environmental science & technology 29 (1995), S. 2073-2082 
    Details
    ISSN: 1520-5851
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/es00008a027
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  • 2
    Electronic Resource
    Electronic Resource
    Phospholipid fatty acid and lipopolysaccharide fatty acid signature lipids in methane-utilizing bacteria (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 85 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The phospholipid fatty acids (PLFA) and lipopolysaccharide (LPS)—hydroxy fatty acids of a variety of methane-utilizing bacterial strains— were analysed by gas chromatography/mass spectrometry. Type I methanotroph PLFA profiles contained a number of unusual monenoic 16- carbon fatty acids. Significant levels of 16: 1ω8c were found in Methylomonas methanica (41%), Methylomonas fodinarum (26%), Methylomonas aurantiaca (30%) and ‘Methylomonas agile’ (14%). High levels of 16: 1ω6c, 16: 1ω5c and 16: 1ω5t were also detected in most Methylomonas and Methylococcus strains. The type II methanotroph ‘Methylosinus trichosporium’ possessed the unusual PLFA 18: 1ω8c as its major polar lipid. Certain type II methanotrophs contained (ω-1)-OH fatty acids with chain lengths varying from 26 to 30 carbon atoms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04693.x
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  • 3
    Electronic Resource
    Electronic Resource
    Colonization and community dynamics of class Flavobacteria on diatom detritus in experimental mesocosms based on Southern Ocean seawater (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 53 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In order to better understand the ecology of microorganisms responsible for secondary production in the Southern Ocean the activity of Flavobacteria communities on diatom detritus in seawater mesocosms was investigated. Seawater was collected from different parts of the Southern Ocean including the Polar Front Zone (PFZ), ice-edge area of the Antarctic Zone (AZ), and a site in the AZ ice pack. Detritus from the cosmopolitan marine diatom Nitzschia closterium Ehrenberg was resuspended in mesocosms containing seawater filtered to remove particulate organic matter, including particle-associated bacteria and most eukaryotes, but retaining native planktonic bacterial assemblages. Mesocosms were incubated at 2 °C and samples analysed for changes in community composition using denaturing gradient gel electrophoresis (DGGE), real-time PCR and fluorescent in-situ hybridization (FISH). DGGE banding patterns and FISH images demonstrated rapid bacterial colonization of the detritus, dominated by members of class γ-Proteobacteria, α-Proteobacteria and Flavobacteria. Real-time PCR data indicated members of class Flavobacteria were involved in initial colonization of detrital aggregate, however relative abundance stayed at similar levels found for the original native particle-associated populations. 16S rRNA gene DGGE banding patterns and sequence analysis demonstrated significant variation in Flavobacteria community structure occurred in the first 20 days of the experiment before community stabilization occurred. The community structures between the three mesocosms also markedly differed and major colonizers were primarily derived from detectable members of the initial particle-associated Flavobacteria community, however the abundant uncultured Flavobacteria agg58 clone-related and DE cluster 2 clades, previously identified in Southern Ocean seawater were not observed to colonize the detritus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2005.01.008
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  • 4
    Electronic Resource
    Electronic Resource
    Ecological and biogeographic relationships of class Flavobacteria in the Southern Ocean (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 51 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The abundance, spatial distribution and diversity of class Flavobacteria were investigated in the Southern Ocean euphotic zone across a latitudinal transect and in the ice pack off Eastern Antarctica. Surface seawater samples filter-fractionated into 0.8 μm particulate and 0.2 m planktonic fractions were investigated with different molecular techniques. The abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteria-specific primers, was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples than in nutrient limited Temperate Zone (TZ) and Sub-Antarctic Zone (SAZ) waters. Abundance of particle-associated Flavobacteria correlated positively with seawater chlorophyll a and nutrient concentrations, suggesting that increased Flavobacteria abundance may relate to enhanced primary production in the PFZ and AZ. This is supported by comparison of DGGE profiles that demonstrated significant differences in the total Flavobacteria community structure and 16S rRNA gene diversity between samples from the PFZ and AZ and those from TZ and SAZ. Sequence analysis revealed a broad diversity amongst class Flavobacteria in the Southern Ocean with several Flavobacteria clades detected in PFZ and AZ waters not detected in TZ and SAZ waters that putatively represent psychrophilic taxa. Sequence data included a large, so far uncultivated, cosmopolitan phylogenetic clade (“DE cluster 2”) that is distributed throughout the Southern Ocean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2004.09.001
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  • 5
    Electronic Resource
    Electronic Resource
    The microbial composition of three limnologically disparate hypersaline Antarctic lakes (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 183 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 16S rRNA clone library analysis was used to examine the biodiversity and community structure within the sediments of three hypersaline Antarctic lakes. Compared to sediment of low to moderate salinity Antarctic lakes the species richness of the hypersaline lake sediments was 2–20 times lower. The community of Deep Lake (32% salinity, average sediment temperature −15°C) was made up almost entirely of halophilic Archaea. The sediment communities of two meromictic hypersaline lakes, Organic Lake (20% salinity, −7°C) and Ekho Lake (15% salinity, 15°C) were more complex, containing phylotypes clustering within the Proteobacteria and Cytophagales divisions and with algal chloroplasts. Many phylotypes of these lakes were related to taxa more adapted to marine-like salinity and perhaps derive from bacteria exported into the sediment from the lower salinity surface waters. The Ekho Lake clone library contained several major phylotypes related to the Haloanaerobiales, the growth of which appears to be promoted by the comparatively high in situ temperature of this lake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb08937.x
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  • 6
    Electronic Resource
    Electronic Resource
    Sterols in a psychrophilic methanotroph, Methylosphaera hansonii (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 186 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A suite of six sterols, lanosterol, lanost-8(9)-en-3β-ol, 4,4-dimethylcholesta-8(14),24-dien-3β-ol, 4,4-dimethylcholest-8(14)-en-3β-ol, 4-methylcholesta-8(14),24-dien-3β-ol and 4-methylcholest-8(14)-en-3β-ol, were identified in the psychrophilic methanotrophic bacterium, Methylosphaera hansonii. Their presence suggests that the capacity for sterol biosynthesis in methanotrophic bacteria is limited to the family Methylococcaceae but which have widely different optimal growth temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09103.x
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  • 7
    Electronic Resource
    Electronic Resource
    A molecular phylogenetic survey of sea-ice microbial communities (SIMCO) (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 35 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 16S rDNA clone library analysis was used to identify bacterial biodiversity in a variety of sea-ice microbial communities (SIMCO). DNA was extracted from seven Antarctic sea-ice samples and one Arctic sea-ice sample and 16S rDNA PCR-amplified using universal and Archaea-specific primers. Recombinant 16S rDNA clones were obtained and dereplicated using restriction fragment length polymorphism analysis (RFLP). After RFLP analysis, 100 distinct phylotypes (a unique clone or group of clones with sequence similarity of 〉0.98) were defined. From the clone libraries 16S rDNA sequences of bacterial and eukaryotic origin were detected, however Archaea were not detected either with universal or Archaea-specific 16S rDNA primer sets. Bacterial phylotypes grouped within the α and γ proteobacteria, the Cytophaga–Flavobacterium–Bacteroides division, the Gram-Positive bacteria and the orders Chlamydiales and Verrucomicrobiales. The majority of bacterial phylotypes were affiliated with heterotrophic taxa and many grouped closely with cultivated genera and species. Eukaryotic clones were affiliated with a variety of autotrophic and heterotrophic nanoplankton and included a large number of chloroplast 16S rDNA genes. The findings of this investigation corroborated culture data indicating bacterial biodiversity increased in SIMCO displaying high levels of primary production, however the bacterial communities within SIMCO were highly heterogeneous at the genus/species-level between different samples. A comparison of Antarctic and Arctic SIMCO revealed certain sea-ice dwelling bacterial genera are common at both poles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00812.x
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  • 8
    Electronic Resource
    Electronic Resource
    Phospholipid fatty acid and lipopolysaccharide fatty acid signature lipids in methane-utilizing bacteria (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 8 (1991), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1991.tb01704.x
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  • 9
    Electronic Resource
    Electronic Resource
    Microbial community variation in pristine and polluted nearshore Antarctic sediments (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 45 (2003), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two molecular methods were used to investigate the microbial population of Antarctic marine sediments to determine the effects of petroleum and heavy metal pollution. Sediment samples were collected in a nested design from impacted and non-impacted locations. A detailed description of the diversity of the microbial population in two samples was obtained using 16S ribosomal DNA clone libraries constructed from an impacted and a non-impacted location. The clone libraries were very similar with the exception of two sequence clusters containing clones from only the impacted location. All samples were analysed by denaturing gradient gel electrophoresis. The band patterns generated were transformed into a presence/absence matrix and a multivariate approach was used to test for differences in the locations. Statistically significant differences were observed both between and within locations. Impacted locations showed a greater variability within themselves than the control locations. Correlations between the community patterns and environmental variables suggested that pollution was one of a number of factors affecting the microbial community composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0168-6496(03)00135-1
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  • 10
    Electronic Resource
    Electronic Resource
    Optimization and maintenance of soluble methane monooxygenase activity inMethylosinus trichosporium OB3b (1994)
    Springer
    Biodegradation 5 (1994), S. 1-11 
    Details
    ISSN: 1572-9729
    Keywords: Methylosinus trichosporium ; methanotroph ; trichloroethene ; methane monooxygenase ; maximization of enzyme activity ; groundwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soluble methane monooxygenase (sMMO) maximization studies were carried out as part of a larger effort directed towards the development and optimization of an aqueous phase, multistage, membrane bioreactor system for treatment of polluted groundwater. A modified version of the naphthalene oxidation assay was utilized to determine the effects of methane:oxygen ratio, nutrient supply, and supplementary carbon sources on maximizing and maintaining sMMO activity inMethylosinus trichosporium OB3b.Methylosinus trichosporium OB3b attained peak sMMO activity (275–300 nmol of naphthol formed h−1 mg of protein−1 at 25°C) in early stationary growth phase when grown in nitrate mineral salts (NMS) medium. With the onset of methane limitation however, sMMO activity rapidly declined. It was possible to define a simplified nitrate mineral salts (NMS) medium, containing nitrate, phosphate and a source of iron and magnesium, which allowed reasonably high growth rates (μmax 0.08 h−1) and growth yields (0.4–0.5 g cells/g CH4) and near maximal activities of sMMO. In long term batch culture incubations sMMO activity reached a stable plateau at approximately 45–50% of the initial peak level and this was maintained over several weeks. The addition of d-biotin, pyridoxine, and vitamin B12 (cyanocobalamin) increased the activity level of sMMO in actively growing methanotrophs by 25–75%. The addition of these growth factors to the simplified NMS medium was found to increase the plateau sMMO level in long term batch cultures up to 70% of the original peak activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695208
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