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  • 1
    Online-Ressource
    Online-Ressource
    Multiscale Fabrication of Functional Materials for Regenerative Medicine. (2011)
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Details
    Schlagwort(e): Regenerative medicine. ; Electronic books.
    Beschreibung / Inhaltsverzeichnis: This book shows that different kinds of signal - chemical, topographical and electrical - can be arranged in a highly controlled way. Using unconventional fabrication techniques, the text uses scale lengths ranging from several micrometers to a few nanometers.
    Materialart: Online-Ressource
    Seiten: 1 online resource (102 pages)
    Ausgabe: 1st ed.
    ISBN: 9783642228810
    Serie: Springer Theses Series
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=798733
    DDC: 571.6
    Sprache: Englisch
    Anmerkung: Intro -- Multiscale Fabrication of Functional Materials for Regenerative Medicine -- Supervisor's Foreword -- Preface -- Acknowledgments -- Contents -- 1 Introduction -- 1.1…The Age of Nanotechnologies -- 1.2…Nanofabrication -- 1.3…Regenerative Medicine and CNS Diseases -- References -- 2 Experimental Techniques -- 2.1…Fabrication Techniques -- 2.1.1 Photolithography -- 2.1.1.1 The Photoresist -- 2.1.1.2 Pre-Baking -- 2.1.1.3 Mask Alignment -- 2.1.1.4 Development and Post-Baking -- 2.1.2 Soft-Lithography -- 2.1.3 Replica Molding -- 2.1.4 MicroMolding in Capillaries -- 2.1.5 Lithographically Controlled Wetting -- 2.2…Characterization Techniques -- 2.2.1 Atomic Force Microscopy -- 2.2.1.1 Working Principle -- 2.2.1.2 The Cantilever -- 2.2.1.3 Operating Modes -- 2.2.2 Scanning Electron Microscopy -- 2.2.2.1 Working Principle -- 2.2.2.2 Sample Coating -- 2.2.3 Optical Microscopy -- 2.2.4 X-Ray Photoelectron Spectroscopy -- References -- 3 Stable Non-Covalent Functionalization of Teflon-AF -- 3.1…Introduction -- 3.2…Laminin Functionalization of Teflon-AF -- 3.3…Laminin Immunofluorescence Assay -- 3.4…Neural Cell Growth on Laminin-Patterned Teflon-AF -- 3.5…Materials and Methods -- 3.5.1 Preparation of Telfon-AF Films -- 3.5.2 Pattern Fabrication on PTFE Surface -- 3.5.3 Characterization of the Patterned Substrates -- 3.5.4 Testing of the Cell Adhesion and Growth -- 3.6…Conclusions -- References -- 4 Stamp-Assisted Multiscale Patterning of TiO2 for Cell Growth Control -- 4.1…Introduction -- 4.2…Fabrication of Porous and Flat TiO2 Patterns -- 4.3…Neural Cell Adhesion and Proliferation on TiO2 Patterns -- 4.4…Neural Cell Density Dependence on Stripe Width -- 4.5…Materials and Methods -- 4.5.1 Colloidal Composite Preparation -- 4.5.2 Titanium Dioxide Pattern Fabrication -- 4.5.3 Titanium Dioxide Pattern Characterization -- 4.5.4 Neural Cell Cultures. , 4.5.5 Neural Cell Adhesion and Proliferation Assays -- 4.5.6 Neural Cell Viability Assay -- 4.5.7 Cytoskeleton and Focal Adhesion Staining by Immunofluorescence Assay -- 4.5.8 Cell Morphology Analysis by Combined SEM, AFM and Optical Microscopies -- 4.5.9 Data Analysis -- 4.6…Conclusions -- References -- 5 Control of Neural Cell Adhesion on 3D-SWCNT -- 5.1…Introduction -- 5.2…Neural Cell Adhesion on SWCNT Pattern -- 5.2.1 Patterning of SWCNT by GAD -- 5.2.2 Cell Adhesion on SWCNT Array -- 5.3…Electric Stimulation of Cells on SWCNT-Based Device -- 5.3.1 Test Bed Fabrication -- 5.3.2 Electric Stimulation -- 5.4…Materials and Methods -- 5.4.1 SWCNT Suspension in O-benzyl Tyrosine and SWCNT/PS Composite -- 5.4.1.1 Patterning of SWCNT by GAD -- 5.4.1.2 Cell Adhesion on SWCNT Arrays -- 5.4.1.3 Fabrication of Test Bed -- 5.4.1.4 Electric stimulation -- 5.4.1.5 Statistical Analysis -- 5.5…Conclusions -- References -- 6 Lithographically Controlled Etching -- 6.1…Introduction -- 6.2…Surface Patterning by Lithographically Controlled Etching -- 6.3…Simulation by Finite Elements of the Lithographically Controlled Etching Process -- 6.4…SiO2 Nanowire Fabrication -- 6.5…One-Pot Surface Patterning and Nanoparticle Deposition -- 6.6…X-Ray Photoelectron Spectroscopy -- 6.7…Absorption Spectroscopy -- 6.8…Materials and Methods -- 6.8.1 Substrate, Stamps and Solutions -- 6.8.2 Atomic Force Microscopy -- 6.8.3 X-Ray Photoelectron Spectroscopy -- 6.9…Conclusions -- 6.10…Outlook of Lithographically Controlled Etching -- References -- 7 Conclusions.
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  • 2
    Digitale Medien
    Digitale Medien
    The ‘petite-negative’ yeast Kluyveromyces lactis has a single gene expressing pyruvate decarboxylase activity (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 19 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: We cloned and sequenced the pyruvate decarboxylase (PDC; EC 4.1.1.1) structural gene KIPDCA in the yeast Kluyveromyces lactis and found it to be allelic to the previously isolated rag6 mutation. The putative amino acid sequence of the KIPdcAp appeared to be highly homologous to those of the yeast Pdc proteins identified so far. The disruption of KIPDCA indicated that it is the only PDC structural gene in K. lactis, as evidenced by the lack of PDC activity and ethanol production in the pdcAΔ strains and by the absence of growth on glucose in the presence of respiratory inhibitors. It was observed that expression of the KIPDCA gene is induced by glucose at the transcriptional level. Transcription of the gene was reduced in the rag1, rag2, rag5 and rag8 mutants, which are defective for the low-affinity glucose permease, phosphoglucose isomerase, hexokinase, and a positive regulator of RAG1 expression, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2958.1996.346875.x
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  • 3
    Digitale Medien
    Digitale Medien
    The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast (2004)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 53 (2004), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: It was observed previously that the deletion of the open reading frame YNL107w (YAF9) was highly pleiotropic in yeast and caused defective growth phenotypes in the presence of several unrelated inhibitors, including caesium chloride. We have selected multicopy extragenic suppressor genes, revealing that this phenotype can be suppressed by overdosing the transcription factors BDF1 and GAT1 in the yaf9Δ strain. We focused our analysis on suppression by BDF1 and performed a genome-wide transcript analysis on a yaf9Δ strain, compared with the wild-type and BDF1-suppressed strains. YAF9 deletion has a clear effect on transcription and leads to modulation of the level of expression of several genes. Transcription of a considerable portion of the underexpressed genes is restored to wild-type levels in the BDF1-suppressed strain. We show by chromatin immunoprecipitation that both Yaf9p and Bdf1p bind to promoters of some of these genes and that the level of H3 and H4 acetylation at one of these promoters is significantly lowered in the yaf9 deleted strain, compared with the wild-type and the BDF1-suppressed strains.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.2004.04184.x
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  • 4
    Digitale Medien
    Digitale Medien
    Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase (2002)
    Oxford, UK : Blackwell Science, Ltd
    Physiologia plantarum 116 (2002), S. 0 
    Details
    ISSN: 1399-3054
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Glutathione transferases (GST; EC 2.5.1.18) have been involved in many biotic and abiotic interactions of plants with their environment, but very little information is available on their regulation and possible role in drought tolerance. The GST8 gene of Arabidopsis thaliana encodes, as assessed by phylogenetic analysis, the homologue of an extremely conserved subgroup of Tau GSTs. During rapid dehydration of seedlings and progressive drought stress of mature plants, GST8 transcript levels increased following slower kinetics than RNAs for dehydration responsive genes RD29A and B, and strictly paralleled, in a mostly ABA independent manner, expression of oxidative stress marker PRX. GST8 RNA levels were also consistently increased by oxidative stress, high doses of auxin or cytokinin, and to a lesser extent, by wounding. RNA levels of ERD13, a previously described rapid dehydration responsive GST of the Phi class, were not co-regulated with those of GST8. Our results suggest that a drought-associated oxidative stress induces accumulation of GST8, whose function could be to counteract the effect of higher ROS production in stressed plants
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1034/j.1399-3054.2002.1160112.x
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  • 5
    Digitale Medien
    Digitale Medien
    The host range of the pKD1-derived plasmids in yeast (1989)
    Springer
    Current genetics 16 (1989), S. 95-98 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Kluyveromyces ; Transformation ; 2μ-like plasmids ; G418 resistance ; pKDI
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary pKDI is a 2μ-like circular plasmid found in the yeast Kluyveromyces drosophilarum that can also stably replicate in Kluyveromyces lactis. We have found a short intergenic region in this genome that appears to be functionally neutral; that is, the introduction of foreign sequences into the single EcoRI restriction site located near one of the inverted repeats did not affect the high stability of the natural plasmid. By introducing a G418 resistance gene at this site, we constructed an autonomous recombinant plasmid. Since this vector did not require cir + hosts for its stable maintenance, it could be used to examine the transformation host range of pKD1 among all the species belonging to the genus Kluyveromyces. Both species closely related to K. drosophilarum as well as a few other species that are very different in chromosomal GC % could be transformed to yield highly stable transformant clones.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00393401
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  • 6
    Digitale Medien
    Digitale Medien
    Active recombination of pKD1-derived vectors with resident pKD1 in Kluyveromyces lactis transformation (1989)
    Springer
    Current genetics 15 (1989), S. 253-260 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Kluyveromyces lactis ; Transformation ; pKD1 ; Recombination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The host specificity of the 2μ-like circular plasmid pKD 1 is such that this plasmid replicates stably in several species of Kluyveromyces yeasts, but not in Saccharomyces cerevisiae. pKD 1-derived plasmids containing various parts of the pKD 1 sequence were capable of transforming Kluyveromyees lactis with high efficiency. When such vectors were introduced into host strains that contained resident pKD1 plasmid, the input DNA frequently recombined with it to produce high proportions of additive recombinant molecules that replicate stably. Recombination events were shown to occur with vectors differing for the presence or absence of the putative origin of replication and of the inverted repeats. Structure, stability and copy number of the recombination products were analyzed for various types of vectors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00447040
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  • 7
    Digitale Medien
    Digitale Medien
    Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 μm circular plasmid pKD1 (1987)
    Springer
    Current genetics 12 (1987), S. 185-192 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Yeast plasmid ; Transformation ; pKD1
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The circular plasmid pKD1 (or 1.6 μm DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 μ DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology. pKD1 can be transferred to Kluyveromyces lactis where it is replicated stably. Using recombinant molecules derived from pKDl, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 μ-based Saccharomyces cerevisiae transformation system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00436877
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  • 8
    Digitale Medien
    Digitale Medien
    Plasmid functions involved in the stable propagation of the pKD1 circular plasmid in Kluyveromyces lactis (1991)
    Springer
    Current genetics 19 (1991), S. 155-161 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Kluyveromyces ; Partitioning ; pKD1 ; Transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Plasmid factors involved in the stable propagation of pKD1-derived vectors in Kluyveromyces lactis transformants have been identified. Three genes (A, B and C) have been found to be present in pKD1: the interruption of the B and C genes led to high plasmid instability. Stability could be restored in trans when host cells contained pKD1 as the resident plasmid (pKD1+ strains). The A gene, which codes for a site-specific recombinase, did not affect plasmid partitioning. Vectors bearing only the pKD1 replication origin (or a chromosomal ARS), and no other pKD1 sequence, were very unstable both in the presence and absence of the resident plasmid in host cells. These vectors could be stabilized in pKD1+ strains, but not in pKD1o strains, by the insertion of a 200 pb-long pKD1 sequence. This sequence, called the cis-acting stability locus (CSL), together with the products of the B and C genes, ensured plasmid partitioning at cell divison. Possible hairpin structures and direct repeats were regularly spaced within the CSL. This region, and the corresponding cis-acting stabilizing elements of other yeast plasmids, did not have sequence homology but shared some structural regularities.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00336481
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  • 9
    Digitale Medien
    Digitale Medien
    TUNA FISH (T-30) – A new proficiency testing material for the determination of As and Hg in seafood (1997)
    Springer
    Fresenius' journal of analytical chemistry 358 (1997), S. 441-445 
    Details
    ISSN: 1432-1130
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The quality of the aquatic and marine environment can be monitored by the determination of pollutants in organisms living in this environment. Certified reference materials and well-organised proficiency tests are powerful means of ensuring a constant level of quality and verifying the correct application of standardised methods. The preparation of a tuna fish proficiency testing material for the evaluation of quality of As and Hg monitoring in seafood is described. Preparation and characterisation of the material as well as studies on its homogeneity and stability are described. Concentrations of 3.4 ± 0.2 mg/kg total arsenic and 2.91 ± 0.09 mg/kg total mercury have been determined as target values. Moreover indicative values for some trace elements (Cd, Cu, Ni, Pb, Sr) and some major constituents (Al, Br, C, Ca, Cl, Fe, H, K, Mg, N, Na, P, S, Si, Zn) have also been measured.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002160050443
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  • 10
    Digitale Medien
    Digitale Medien
    Expression of zein in long term cultures of wildtype and opaque-2 maize endosperms (1989)
    Springer
    Plant cell reports 7 (1989), S. 639-643 
    Details
    ISSN: 1432-203X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, our data indicate a severe restriction in zein accumulation, resulting both from reduction of transcription and from post-transcriptional events. No detectable zein proteins and trace amounts of transcripts were found in opaque-2 cultures, which do not therefore exhibit a distinctive opaque-2 phenotype.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00272049
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