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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructure of mast cells in the hyperplastic buccal mucosa of the zinc-deficient rat (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 14 (1985), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: As previously reported, young rats on zinc-deficient diets develop a marked epithelial hyperplasia in the mucosa of the cheek, associated with a four-fold increase in the number of mast cells in the lamina propria. We now report the results of a quantitative and qualitative ultrastructural study of these mast cells. In contrast to the cells in mast cell-related disease, the cells were of normal morphology, but of consistently increased size, containing proportionally increased numbers of mitochondria and specific granules. The size distribution of the granules was as in controls. Microvilli were lengthened in proportion to the increase in the cell circumference. The number of nuclear pores was increased but their spacing was as in controls. In all these measurements, the experimental mast cells were a remarkably homogeneous population. We conclude that mast cells are capable of developing into cells of larger size showing evidence of greater activity. It may be that this development occurs in response to the same (unknown) stimulus which causes their number to increase. By virjuc of their increased number and size, mast cells may play an important role in the enhancement of the microcirculation commonly seen in conditions of epithelial hyperplasia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1985.tb00508.x
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  • 2
    Electronic Resource
    Electronic Resource
    Surface ultrastructural changes in chemically induced premalignant lesions in the hamster cheek pouch (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 16 (1987), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The histopathological and topographical alterations occurring during the early stages of chemical carcinogenesis in the hamster cheek pouch using 7, 12-dimethylbenz (α) anthracene (DMBA) were studied by light and scanning electron microscopy. At the scanning electron microscopic level, cellular overlapping and mild disturbance of intercellular bridges were noticed as early as the second week of DMBA application; these changes progressed with time. Clear epithelial dysplastic changes at the light microscopic level were detected by the sixth week of the experiment. The results of this investigation demonstrate the usefulness of scanning electron microscopy as an adjunct tool to study early alterations in cell morphology which occur in the stratified squamous epithelium of the hamster cheek pounch in response to a chemical carcinogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1987.tb01492.x
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  • 3
    Electronic Resource
    Electronic Resource
    Use of x-ray microanalysis to monitor diffusion of Zn from a cement into human dentin (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 12 (1983), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Energy dispersive x-ray spectrometry (EDAX system), with scanning electron microscopy was used to detect diffusion of Zn from a source in human dentin and the system was tested for sensitivity and reproducibility. Sections of human teeth in which a ZnO-eugenol cement had been placed in a cavity in the dentin in vivo were prepared and x-ray microanalytical measurements carried out. We found that values of counts for Zn, Ca and P in the same field were linear up to a total counting time of 60 sec. We further found that reproducibility of values between repeat examinations of the same specimens on different days was excellent. The concentration of Zn in the dentin decreased exponentially with distance from source within the distance tested. We conclude that the system can be used to detect trace concentrations of Zn, and that accordingly the diffusion behavior of a variety of ions in dentin could be monitored.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1983.tb00333.x
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  • 4
    Electronic Resource
    Electronic Resource
    The hepatic ultrastructure of chlordene-fed pigeons (1977)
    Springer
    Archives of environmental contamination and toxicology 5 (1977), S. 385-402 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Adult male pigeons were fed 500 ppm of nontoxic chlordene per day for 6 weeks. The ultrastructure of hepatic cells of treated pigeons was examined and compared with that of the control birds. Chlordene feeding induced cytological changes in the structure of liver cells. Thinning of the microvilli of bile canaliculi and clear marking of fibrous long spacing and non-fibrous segment long spacing on collagen fibers were noticed. Increase in the number as well as the vacuolation of lipid bodies in certain portions of the cytoplasm, presence of myeloid bodies in mitochondria, and an increase in the smooth-surfaced endoplasmic reticulum were observed in the liver cells of pigeons given chlordene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02220919
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Calcium distribution in freeze-dried enamel organ tissue during normal and altered enamel mineralization (1984)
    Springer
    Calcified tissue international 36 (1984), S. 596-603 
    Details
    ISSN: 1432-0827
    Keywords: Calcium ; Ameloblasts ; X-ray microanalysis ; Transport ; Frozen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Energy dispersive X-ray microanalysis was applied to freeze-dried blocks of enamel organ tissue to determine levels of calcium in various celular regions. The tissue blocks were dissected free from adjacent forming enamel following injection of cobalt or fluoride ions, both of which temporarily inhibit enamel mineralization. In all control and experimental specimens there was an increasing gradient of calcium from the stratum intermedium cells to the distal ends of the ameloblasts. Calcium levels were significantly reduced near the distal ends of the ameloblasts following cobalt or fluoride injection as compared with controls. It is suggested that evidence of an intercellular buildup of calcium near the distal ends of the ameloblast supports a controlling function of these cells. The changes in calcium levels are correlated with alterations in mineralization known to occur in the adjacent enamel of the model systems employed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405373
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  • 6
    Electronic Resource
    Electronic Resource
    Staining of oral epithelium with the zinc iodide-osmium reaction (1981)
    Springer
    Journal of molecular histology 13 (1981), S. 45-55 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Some of the parameters affecting the staining of keratinized oral epithelium with the zinc iodide-osmium reaction were examined using light and electron microscopy and electron probe microanalysis. Factors examined were block size, incubation temperature and the effect of aldehyde prefixation. Large blocks (4 mm cube) were subdivided after incubation and the staining of the centre and edge compared. Generally the reaction was more variable at the edge than in the centre. Small blocks (1 mm cube) showed a more intense reaction when incubated at 24°C than at 4°C. In all these preparations, final reaction product was seen over Golgi systems, lysosome-like bodies, membrane-coating granules and, in the more intensely stained regions, over endoplasmic reticulum and nuclear membranes as well. In prefixed material, mitochondria were frequently stained in addition to the other organelles. Energy dispersive analysis showed the reaction product to be similar in all preparations and to contain high levels of zinc and osmium but not iodine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01005838
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