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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 789 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Key words SHR ; Membrane lipid composition ; Membrane fluidity ; D-glucose transport ; SGLT-1 ; Brush-border membrane vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The current studies explore the effect of hypertension on D-glucose transport into jejunal brush-border membrane vesicles (BBMV). Spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats, as a control group, were used. The purity of the BBMV from both groups of animals was validated by the finding that the specific activity of brush-border enzyme marker, sucrase, was severalfold greater in membrane vesicles compared with corresponding values in mucosal homogenate. D-glucose uptake was Na+ dependent in both groups of animals, with a transient increase in the intravesicular concentration of D-glucose. However, the initial rate and the magnitude of the accumulation of Na+-dependent D-glucose was significantly higher in SHR compared with WKY rats. In order to investigate the mechanism(s) for the increase in Na+-dependent D-glucose transport in SHR, several experiments were performed: (1) an experiment that indicated 22Na uptake, as an indicator for Na+ permeability, was similar between SHR and WKY rats, (2) kinetic studies that indicated that V max values of SHR were significantly greater that those of WKY rats. In contrast, similar K m values for glucose were found between SHR and WKY rats, (3) Na+-dependent phlorizin binding measurements that were not altered by hypertension and (4) a study of the brush-border membrane lipid composition that showed a significant increase in the free cholesterol/phospholipid ratio in SHR. We conclude that altered membrane cholesterol content and consequently altered lipid fluidity could be, at least in part, responsible for the observed increase in Na+-dependent D-glucose transport in SHR.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 34 (1989), S. 1713-1719 
    ISSN: 1573-2568
    Keywords: large intestine ; diarrhea ; small bowel resection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of intestinal resection on several intestinal parameters have been studied in the large intestine of rats one month after the surgical operation. The results show that both 50% and 80% distal small bowel resection increased net fluid absorption and mucosal permeability and caused expansion of the intercellular spaces of the large intestine. The increase in net fluid absorption was dependent upon the extent of the intestine removed. The cAMP and cGMP content of cecal and colonic mucosa were significantly reduced after jejunoilectomy. Changes in nucleotide levels were dependent on the length of the intestine resected. On the other hand, mucosa Na,K-ATPase specific activity was only increased in the cecum after 80% intestinal resection. The results are discussed in terms of adaptation to prevent diarrhea.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-4935
    Keywords: Spontaneously hypertensive rat ; brush-border membrane ; lipid fluidity ; lipid composition ; D-glucose ; Na+-H+ exchanger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The lipid composition and fluidity of jejunal brush-border membrane vesicles (BBMV) have been studied in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats. The activities of both Na+-dependent D-glucose cotransport and Na+-H+ antiport have also been determined. A significant increase in the level of free cholesterol was observed in jejunal BBMV from SHR compared to WKY rats. Since phospholipid values did not change in either group of animals, a significant enhancement in the free cholesterol/phospholipid ratio was observed in SHR. A decrease in the levels of phosphatidylethanolamine together with an increase in the values of phosphatidylserine was observed in hypertensive rats. Although the content of phosphatidylcholine (PC) and sphingomyelin (SM) was not singificantly altered in SHR, the ratio PC/SM significantly increased in these animals when compared to WKY rats. The major fatty acids present in bursh-border membranes prepared from SHR and WKY rats were palmitic (16∶0), stearic (18∶0), oleic (18∶1, n-9) and linoleic (18∶2, n-6), and the fatty acid composition was not modified by the hypertension. A decreased fluorescence polarization, i.e., increased membrane fluidity, was observed in SHR, which was not correlated to the increased ratio of cholesterol/phospholipid found in the brush-border membrane isolated from these animals. These structural changes found in SHR were associated to an enhancement in both Na+-dependent D-glucose transport and Na+-H+ antiport activity in the jejunal BBMV of SHR.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-4935
    Keywords: oleic acid ; olive oil ; lipid composition ; hydrolases ; jejunum ; rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of two monounsaturated fatty acid (MUFA) oils, olive oil (OO)and high-oleic sunflower oil (HOSO), with high content in oleic acid butdiffering in their non-fatty acid fraction, on brush-border membrane(BBM) lipid composition and fluidity and on mucosal enzyme activitiesof rat jejunum were studied. Animals were given semipurified diet withlinoleic acid to prevent essential fatty acid deficiency (control group)or semipurified diet containing 10% of either OO or HOSO for 12weeks. There was a significant decrease in the content of jejunalBBM phospholipids together with an increase in the level of freecholesterol in both oil-fed rats, when compared to controlgroup. Although the increase in the BBM free cholesterol levelwas not statistically significant in HOSO-fed rats, a significantdecrease in the phospholipid/free cholesterol ratio was found inboth OO and HOSO-fed animals compared to control group. Rat jejunalBBM had a high level of free fatty acids which was increased in BBMisolated from OO and HOSO-fed animals. There was no statisticalsignificant difference in the phospholipid distribution between thecontrol and the OO group. However, HOSO-fed animals showed the lowestlevel of phosphatidylethanolamine together with the highestphosphatidylcholine content and the phosphatidylcholine/sphingomyelinratio. The fatty acid pattern of jejunal BBM lipids was modifiedaccording to the major fatty acids in the oils. There was a decreasein both stearic acid (18:0) and linoleic acid (18:2 n-6), togetherwith an increase in oleic acid (18:1 n-9) in jenunal BBM isolatedfrom both oil experimental groups. All these results were accompaniedby a significant increase in the BBM fluidity (as assessed bysteady-state fluorescence polarization of diphenylhexatriene) isolatedfrom oil-fed rat, when compared to control group. OO and HOSO-fedanimals had the lowest activities of sucrase and maltase, whilealkaline phosphatase activity only was decreased in HOSO-fedanimals. The specific activity of maltase was not modified in anyexperimental rats. In summary, both MUFA oils induced similar effectson jejunal BBM lipid composition, fluidity, sucrase, maltase andlactase activities. Furthermore, HOSO intake resulted in a lowestalkaline phosphatase activity which was accompanied by changes inindividual phospholipid composition. All these results suggest thateffects of MUFA oils on jejunal BBM lipid composition and hydrolaseactivities are most likely due to the presence of high content ofoleic acid rather than other components contained in the non-fattyacid of olive oil.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Polymer bulletin 27 (1992), S. 527-534 
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract A polyester containing diacetylenic units in main chain was prepared by the polycondensation of m, m′-butadiynylene dibenzyl alcohol with terephthaloyl chloride. The polymer was crystalline, and bearly soluble in hot dimethylformamide or dimethylsulfoxide, but insoluble in most organic solvents. It melts at about 200°C. Irradiation of UV light at 75°C gave a reddish brown resin. Heating at temperatures above its melting point gave a shinning black hard solid.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary Copolymers of methyl methacrylate(MMA) with N,N-dimethylaminopropylacrylamide (DMA) were heated at various temperatures, and their thermal crosslinking was investigated. A copolymer with a compsition of [MMA]/[DMA] =3 became completely insoluble in chloroform when heated at 180°C for 5 minutes or at 150°C for 20 minutes. Other copolymers containing less than 5 mol % DNA also crosslinked when heated at 170°C for 30 minutes. A mixture of polyMMA with N,N-dimethylaminopropylbenzamide also crosslinked when heated at 170°C. A copolymer of styrene with DMA also crosslinked when heated at above 140°C although the crosslinking was much slower than that of poly(MMA-co-DMA). The formation of imide linkages is proposed as the crosslinking mechanism.
    Type of Medium: Electronic Resource
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  • 8
    Publication Date: 2021-05-11
    Description: The understanding of earthquake physics is hindered by the poor knowledge of fault strength and temperature evolution during seismic slip. Experiments reproducing seismic velocity (∼1 m/s) allow us to measure both the evolution of fault strength and the associated temperature increase due to frictional heating. However, temperature measurements were performed with techniques having insufficient spatial and temporal resolution. Here we conduct high velocity friction experiments on Carrara marble rock samples sheared at 20 MPa normal stress, velocity of 0.3 and 6 m/s, and 20 m of total displacement. We measured the temperature evolution of the fault surface at the acquisition rate of 1 kHz and over a spatial resolution of ∼40 µm with an optical fiber conveying the infrared radiation to a two‐color pyrometer. Temperatures up to 1,250°C and low coseismic fault shear strength are compatible with the activation of grain size dependent viscous creep.
    Description: Published
    Description: e2020GL091856
    Description: 3T. Fisica dei terremoti e Sorgente Sismica
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 9
    Publication Date: 2024-04-11
    Description: Dynamic fault strength τ (rock friction in the broad sense) and its evolution with seismic slip and slip rate are among the most relevant parameters in earthquake mechanics. Given the large slip rate (1 m s−1 on average), displacement (up to tens of meters), effective stress (tens of MPa), typical of seismic faulting at depth, thermo-mechanical effects become outstanding: dynamic fault strength is severely affected by fluid and rock phase changes, extreme grain size reduction, and the production of amorphous and unstable materials in the slipping zone. Here, first we will summarize the most relevant findings about dynamic fault strength during seismic slip mainly obtained thanks to the exploitation of dedicated experimental machines (i.e., rotary shear apparatus). However, the interpretation of this experimental dataset remains debated because of technical limitations which impede us to measure fundamental parameters such as temperature, strain rate, pore fluid pressure and grain size in the slipping zone. Without a sound estimate of these physical parameters, any constitutive law proposed to describe the evolution of dynamic fault strength during simulated seismic slip remains speculative. Then, we will discuss the results of some recent experiments which exploit new technical approaches to overcome the main limitations of the previous studies. The experimental approach, together with field studies of the geometry and architecture of exhumed faults and modelling, remains our most powerful tool to investigate seismic-related deformation mechanisms in both natural and human-induced earthquakes.
    Description: Unpublished
    Description: ISRM Regional Symposium - 11th Asian Rock Mechanics Symposium October 21–25, 2021 Beijing, China
    Description: OST3 Vicino alla faglia
    Keywords: earthquakes ; rock mechanics ; structural geology
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Conference paper
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  • 10
    Publication Date: 2016-07-20
    Description: Nature Immunology 17, 985 (2016). doi:10.1038/ni.3504 Authors: Danay Cibrian, María Laura Saiz, Hortensia de la Fuente, Raquel Sánchez-Díaz, Olga Moreno-Gonzalo, Inmaculada Jorge, Alessia Ferrarini, Jesús Vázquez, Carmen Punzón, Manuel Fresno, Miguel Vicente-Manzanares, Esteban Daudén, Pedro M Fernández-Salguero, Pilar Martín & Francisco Sánchez-Madrid
    Print ISSN: 1529-2908
    Electronic ISSN: 1529-2916
    Topics: Biology , Medicine
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