ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The intracellular development of reovirus type 2 and its interaction with an established human amnion cell line (RA) have been investigated. Adsorption studies indicate that maximal adsorption of the virus to the RA cell was attained within 75–90 minutes after exposure. Single cycle growth studies revealed an eclipse period of 9 hours, and maximal yields were reached at 36 hours post-infection (p.i.). In comparison, the eclipse period for reovirus type 3 in the same cell line was 6 hours, with maximal yields attained at approximately 26 hours p.i. Immunofluorescent and cytochemical (acridine orange) examination of virus-induced alterations in the cell during a single growth cycle of reovirus type 2 revealed the following: a) appearance of virus antigen in the perinuclear region as early as 4 hours p.i. and a maximum number of cells containing antigen by 10 hours p.i.; b) appearance of orthochromatically green-staining perinuclear inclusions at 6 hours p.i. These inclusions were resistant to digestion by ribonuclease and deoxyribonuclease. Metachromatically red-staining inclusions sensitive to ribonuclease were observed to appear in a few cells late in the infectious cycle.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01241697
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