Publication Date:
2024-04-03
Description:
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B(12) and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B(12)-replete conditions, cells grown at high pCO(2) had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO(2). At high pCO(2), B(12)-limited cells had ~50% lower specific growth and carbon fixation rates, and used Fe ~15-fold less efficiently, and Zn and Cd ~3-fold less efficiently, in comparison with B(12)-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO(2)/B(12)-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B(12)-replete cells were 5- to 14-fold higher in comparison with B(12)-limited cells, suggesting that 〉80% of cellular Co of B(12)-limited cells was likely from B(12). Our results demonstrate that CO(2) and vitamin B(12) interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean.
Keywords:
Alkalinity, total; Aragonite saturation state; Attheya sp.; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Cadmium/Phosphorus ratio; Cadmium/Phosphorus ratio, standard deviation; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon/Phosphorus ratio; Carbon/Phosphorus ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chromista; Cobalt/Phosphorus ratio; Cobalt/Phosphorus ratio, standard deviation; Coulometric titration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Iron/Phosphorus ratio; Iron/Phosphorus ratio, standard deviation; Laboratory experiment; Laboratory strains; Micro-nutrients; Net use efficiency, Cadmium; Net use efficiency, Cadmium, standard deviation; Net use efficiency, Cobalt; Net use efficiency, Cobalt, standard deviation; Net use efficiency, Iron; Net use efficiency, Iron, standard deviation; Net use efficiency, Zinc; Net use efficiency, Zinc, standard deviation; Nitrogen/Phosphorus ratio; Nitrogen/Phosphorus ratio, standard deviation; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phytoplankton; Potentiometric; Primary production; Primary production/Photosynthesis; Primary production of carbon, standard deviation; Salinity; Single species; Species; Temperature, water; Treatment; Zinc/Phosphorus ratio; Zinc/Phosphorus ratio, standard deviation
Type:
Dataset
Format:
text/tab-separated-values, 270 data points
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