ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The study of the effect of different carbon sources reflected the following results: 1. Candida parapsilosis (wild type and mutant) could not utilize citrate and glyoxylate and could grow well on pyruvate, acetate and to some extent on α-ketoglutarate, trans-aconitate, isocitrate and succinate. 2. Cells grown on tetradecane and pentadecane formed much more pseudomycelium than cells grown on the other tested carbon sources. 3. The catalase activities of the cells grown on tetradecane were generally much higher than those of the cells grown on acetate or glucose. 4. The tetradecane-grown cells of both organisms contain more lipid than glucose-, acetate- or succinate-grown cells. 5. Among the fatty acids found in the cells, the even-numbered dominate. A few odd-numbered were detected. 6. Enzymes of the glyoxylate cycle had very high activities on acetate and tetradecane, but very low ones on glucose and succinate medium. The two strains had with all the carbon sources a high citrate synthase activity. 7. High concentrations of glucose (2.5% and more) repressed the enzyme activities tested while acetate abolished this effect. Many authors have reported the fermentative production of citric acid from hydrocarbons (Nakanishi et al. 1972; Puklowski et al. 1976; Schröder 1976), the effect of hydrocarbons on the morphology of n-alkane degrading yeasts (Hirai et al. 1972), the effect of substrate on the fatty acid composition of hydrocarbon utilizing microorganisms (Dunlap and Perry 1967; Mishina et al. 1973; Source on enzymes of the tricaboxylic acid- and glyoxylate cycle (Gosling and Duggan 1971; Lozinov et al. 1976; Kleber and Aurich 1973, 1974; Fritsche 1968; Nabeshina et al. 1977), and catalase activity of hydrocarbon metabolizing yeasts (Teranishi et al. 1974a, b). Each author used a different yeast strain and no work has reported these different physiological studies for one organism at a time. In a previous work we reported the effect of pH, temperature, age and trans-aconitate on growth and enzymes of Candida parapsilosis (Omar and Rehm 1980). The following paper deals with experiments on the utilization of various carbon sources and on the comparison of enzyme activities of the TCC and glyoxylate cycle, catalase and fatty acid composition of C. parapsilosis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00514077
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