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  • 1
    Keywords: Cell biology. ; Microbiology. ; Fluoreszenz-in-situ-Hybridisierung
    Description / Table of Contents: An Introduction to Fluorescence in situ Hybridization in Microorganisms -- FISH Variants -- Bioinformatic Tools and Guidelines for the Design of Fluorescence in situ Hybridization Probes -- FISH in Suspension or in Adherent Cells -- Application of Nucleic Acid Mimics in Fluorescence in situ Hybridization -- Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes -- Characterisation of Social Interactions and Spatial Arrangement of Individual Bacteria in Multi-Strain or Multispecies Biofilm Systems using Nucleic acid mimics-Fluorescence in situ Hybridization -- Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant Surfaces -- CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples -- Fluorescence in Situ Hybridization with Quantum Dot Labels in E. coli Cells -- Monitoring Bacteriophage Infection on Bacterial Cells using FISH -- Linking Microbes to their Genes at Single Cell Level with direct–geneFISH -- Assigning Function To Phylogeny: FISH-nanoSIMS -- Assigning Function to Phylogeny: MAR-FISH -- Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-based Fluorescence in situ hybridization (fliFISH) -- Integration of FISH and Microfluidics -- Flow-FISH using Nucleic Acid Mimic Probes for the Detection of Bacteria -- FISH in Food Samples -- Extraction of Microbial Cells from Environmental Samples for FISH Approaches -- Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology -- Computational Resources and Strategies to Construct Single-Molecule Models of FISH.
    Type of Medium: Book
    Pages: xii, 334 Seiten , Illustrationen
    ISBN: 9781071611142
    Series Statement: Methods in molecular biology 2246
    Language: English
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 23 (1982), S. 155-160 
    ISSN: 1432-1041
    Keywords: amikacin ; pharmacokinetics ; development ; neonate ; infant ; child
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The disposition kinetics of a single i.v. dose of amikacin was studied in 6 neonates (6–25 days old), 10 infants (4–18 months) and 8 young children (3–11 years). There was a progressive change in the distribution and elimination kinetics during development. The distribution coefficient of the antibiotic averaged of 0.429, 0.320 and 0.210 l/kg in the newborns, infants and young children, respectively and serum half-life (t1/2 β) in these three groups averaged 2.812, 1.803 and 1.196 h, respectively. Significant differences in certain pharmacokinetic parameters were found between the values in paediatric patients and in adults receiving the same dose. A linear relationship was established between the distribution volume of the antibiotic and the weight of the patients, as defined by the following equation: $${\text{Vd}}_{{\text{ss}}} \left( 1 \right) = 0.976 + 1.140 \cdot {\text{TBW}}\left( {{\text{kg}}} \right);r = 0.954$$ The results suggest that a regimen of very frequent administrations should be employed in infants and young children in order to maintain a therapeutic level throughout treatment.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1433-7339
    Keywords: Key words Portugal ; Cancer ; Disclosure ; Questionnaire ; Truth-telling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Disclosure of the diagnosis of cancer to the patients affected has always been a controversial issue in the doctor–patient relationship. Undoubtedly this is so not only because of differences between countries and cultures, but also because there have been changes of opinion over the years. The aim of this study was to evaluate the quality and quantity of information desired by Portuguese cancer patients, and how and from whom they want to hear this information. Our sample comprised a total of 193 cancer patients, 87 men and 106 women. We found that 68.9% knew what their diagnosis was. In our sample, 74% wanted "as much information as possible, good or bad"; 85% said they wanted to know if their disease was cancer; 95% wanted to know the best or worst likely outcome of their disease; and 96.4% wanted to know the chances of getting cured. Most patients said they would prefer to be informed by physicians (92.7%) and have access to a telephone helpline, books and television. In conclusion, most patients wanted to know as much as possible about their illness and treatment, and the majority preferred to be involved in treatment decisions.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2022-05-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in A stalagmite test of north atlantic SST and iberian hydroclimate linkages over the last two glacial cycles. Climate of the Past, 14(12), (2018); 1893-1913., doi:10.5194/cp-14-1893-2018.
    Description: Close coupling of Iberian hydroclimate and North Atlantic sea surface temperature (SST) during recent glacial periods has been identified through the analysis of marine sediment and pollen grains co-deposited on the Portuguese continental margin. While offering precisely correlatable records, these time series have lacked a directly dated, site-specific record of continental Iberian climate spanning multiple glacial cycles as a point of comparison. Here we present a high-resolution, multi-proxy (growth dynamics and δ13C, δ18O, and δ234U values) composite stalagmite record of hydroclimate from two caves in western Portugal across the majority of the last two glacial cycles (∼220 ka). At orbital and millennial scales, stalagmite-based proxies for hydroclimate proxies covaried with SST, with elevated δ13C, δ18O, and δ234U values and/or growth hiatuses indicating reduced effective moisture coincident with periods of lowered SST during major ice-rafted debris events, in agreement with changes in palynological reconstructions of continental climate. While in many cases the Portuguese stalagmite record can be scaled to SST, in some intervals the magnitudes of stalagmite isotopic shifts, and possibly hydroclimate, appear to have been somewhat decoupled from SST.
    Description: This work was supported by the Center for Global and Regional Environmental Research, Cornell College (to Rhawn F. Denniston), and the US National Science Foundation (grant BCS-1118155 to Jonathan A. Haws, BCS-1118183 to Michael M. Benedetti, and AGS-1804132 to Caroline C. Ummenhofer). Field sampling was performed under the auspices of IGESPAR (to Jonathan A. Haws) and Associação de Estudos Subterrâneos e Defesa do Ambiente. Brandon Zinsious and Stephen Rasin contributed to fieldwork at BG, and Zachary LaPointe assisted with radioisotopic analyses; Suzanne Ankerstjerne performed stable isotope measurements. This paper benefitted tremendously from discussions with Maria F. Sánchez Goñi, David Hodell, and Chronis Tzedakis. We thank five anonymous reviewers who substantially improved this paper's scope and clarity through detailed and thoughtful assessments. Stable and U-series isotope data are available at the NOAA National Centers for Environmental Information website.
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 5
    Publication Date: 2016-04-14
    Description: The Journal of Physical Chemistry B DOI: 10.1021/acs.jpcb.5b12544
    Electronic ISSN: 1520-5207
    Topics: Chemistry and Pharmacology , Physics
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  • 6
    Publication Date: 2018-09-25
    Description: Author(s): Justin Walker, Stanislav Boldyrev, and Nuno F. Loureiro It was proposed recently by Loureiro and Boldyrev [ Phys. Rev. Lett. 118 , 245101 (2017) ] and Mallet et al. [ Mon. Not. R. Astron. Soc. 468 , 4862 (2017) ] that strongly anisotropic current sheets formed in the inertial range of magnetohydrodynamic turbulence become affected by the tearing instability at... [Phys. Rev. E 98, 033209] Published Mon Sep 24, 2018
    Keywords: Plasma Physics
    Print ISSN: 1539-3755
    Electronic ISSN: 1550-2376
    Topics: Physics
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  • 7
    Publication Date: 2018-03-06
    Description: Author(s): Daniel Grošelj, Alfred Mallet, Nuno F. Loureiro, and Frank Jenko We present results from a three-dimensional particle-in-cell simulation of plasma turbulence, resembling the plasma conditions found at kinetic scales of the solar wind. The spectral properties of the turbulence in the subion range are consistent with theoretical expectations for kinetic Alfvén wave... [Phys. Rev. Lett. 120, 105101] Published Mon Mar 05, 2018
    Keywords: Plasma and Beam Physics
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
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  • 8
    Publication Date: 2017-06-17
    Description: Author(s): Nuno F. Loureiro and Stanislav Boldyrev The current understanding of magnetohydrodynamic (MHD) turbulence envisions turbulent eddies which are anisotropic in all three directions. In the plane perpendicular to the local mean magnetic field, this implies that such eddies become current-sheetlike structures at small scales. We analyze the r… [Phys. Rev. Lett. 118, 245101] Published Fri Jun 16, 2017
    Keywords: Plasma and Beam Physics
    Print ISSN: 0031-9007
    Electronic ISSN: 1079-7114
    Topics: Physics
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  • 9
    Publication Date: 2014-12-11
    Description: Author(s): César Bernardo, Nuno F. Castro, Miguel C. N. Fiolhais, Hugo Gonçalves, André G. C. Guerra, Miguel Oliveira, and António Onofre The Wtb vertex structure and the search for new anomalous couplings is studied using top quark measurements obtained at the LHC, for a center-of-mass energy of 8 TeV. By combining the latest and most precise results on the single top quark production cross section and the measurements of the W-boson... [Phys. Rev. D 90, 113007] Published Wed Dec 10, 2014
    Keywords: Electroweak Interactions
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
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  • 10
    Publication Date: 2016-10-22
    Description: The properties of plasma turbulence in a poloidally limited scrape-off layer (SOL) are addressed, with focus on ISTTOK, a large aspect ratio tokamak with a circular cross section. Theoretical investigations based on the drift-reduced Braginskii equations are carried out through linear calculations and non-linear simulations, in two- and three-dimensional geometries. The linear instabilities driving turbulence and the mechanisms that set the amplitude of turbulence as well as the SOL width are identified. A clear asymmetry is shown to exist between the low-field and the high-field sides of the machine. While the comparison between experimental measurements and simulation results shows good agreement in the far SOL, large intermittent events in the near SOL, detected in the experiments, are not captured by the simulations.
    Print ISSN: 1070-664X
    Electronic ISSN: 1089-7674
    Topics: Physics
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