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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 253 (2005), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Escherichia coli with various types of adherence patterns to cultured epithelial cells have been described over the years as being associated with both acute and persistent diarrhea. Most enteroaggregative E. coli (EAEC) strains harbor a 60- to 65-MDa plasmid called pAA which has been shown to encode the aggregative adherence fimbriae AAF/I and AAF/II; the enterotoxin EAST1 and Pet, a serine protease which has been described as causing enterotoxic and cytotoxic effects. Another serine protease denominated Pic, encoded by a chromosomal gene displaying mucinolytic activity, serum resistance, and hemagglutination, has also been associated with EAEC strains. In this study, EAEC strains that isolated from the rectal swab of neonates at the neonatal intensive care unit of Pusan National University Hospital in 2003 were tested for the presence of the pAA using polymerase chain reaction (PCR) and DNA colony hybridization methods. To further characterize these EAEC strains, we used PCR to detect genes for proposed EAEC virulence factors and examined HeLa cell adherence assay, antimicrobial susceptibility test, serotyping, cytotoxicity test and epidemiological characteristics. EAEC isolates found were genotyped by random amplified polymorphic DNA and pulsed-field gel electrophoresis.
    Type of Medium: Electronic Resource
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  • 2
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    AGU (American Geophysical Union) | Wiley
    In:  Geophysical Research Letters, 43 (10). pp. 5243-5251.
    Publication Date: 2021-04-21
    Description: The temporal dynamics of the concentrations of nitrate (N), phosphate (P), and the N:P ratio in the upper water column (200-600m) of the Mediterranean (MED) Sea were investigated using observational data (~123,100 data points) collected between 1985 and 2014. The studied variables were found to evolve similarly in the western and eastern MED Sea. In both basins, the N concentration increased during the first part of the observational period (1985-1998), and the temporal trend of N was broadly consistent with the history of riverine and atmospheric nitrogen input from populated areas in Europe, with a lag period of 20years. In subsequent years, the N concentration was high and relatively constant between 1998 and 2005, after which N decreased gradually, although the decreasing trend was indistinct in the western basin. In particular, the trend of constant then declining N after 1998 is consistent with the history of pollutant nitrogen emissions from the European continent, allowing a 20 year lag following the introduction of regulation of pollutant nitrogen in the 1970s. The three-phase temporal transition in P in both basins was more consistent with the riverine phosphorus input, with a lag period of 20years. Our analysis indicates that the recent dynamics of N and P in the upper MED Sea has been sensitive to the dynamics of anthropogenic nitrogen and phosphorus input from atmospheric deposition and rivers
    Type: Article , PeerReviewed
    Format: text
    Format: text
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