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  • 1
    Online Resource
    Online Resource
    Molecular Approaches to Crop Improvement. (1991)
    Vienna :Springer Wien,
    Details
    Keywords: Crops-Genetic engineering. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (176 pages)
    Edition: 1st ed.
    ISBN: 9783709191088
    Series Statement: Plant Gene Research Series
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6503173
    Language: English
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    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    Study of chorismate mutase-prephenate dehydrogenase in crude cell extracts of Escherichia coli (1979)
    s.l. : American Chemical Society
    Biochemistry 18 (1979), S. 4707-4714 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00588a035
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid (1989)
    Springer
    Plant molecular biology 13 (1989), S. 533-540 
    Details
    ISSN: 1573-5028
    Keywords: 2,4-dichlorophenoxyacetic acid (2,4-D) ; detoxification ; herbicide resistance ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were produced through the genetic engineering of a novel detoxification pathway into the cells of a species normally sensitive to 2,4-D. We cloned the gene for 2,4-D monooxygenase, the first enzyme in the plasmid-encoded 2,4-D degradative pathway of the bacterium Alcaligenes eutrophus, into a cauliflower mosaic virus 35S promoter expression vector and introduced it into tobacco plants by Agrobacterium-mediated transformation. Transgenic tobacco plants expressing the highest levels of the monooxygenase enzyme exhibited increased tolerance to 2,4-D in leaf disc and seed germination assays, and young plants survived spraying with levels of herbicide up to eight times the usual field application rate. The introduction of the gene for 2,4-D monooxygenase into broad-leaved crop plants, such as cotton, should eventually allow 2,4-D to be used as an inexpensive post-emergence herbicide on economically important dicot crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027313
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The sequence of a pea vicilin gene and its expression in transgenic tobacco plants (1988)
    Springer
    Plant molecular biology 11 (1988), S. 683-695 
    Details
    ISSN: 1573-5028
    Keywords: vicilin gene ; transgenic tobacco ; Agrobacterium tumefaciens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 5.5 kb Eco RI fragment containing a vicilin gene was selected from a Pisum sativum genomic library, and the protein-coding region and adjacent 5′ and 3′ regions were sequenced. A DNA construction comprising this 5.5 kb fragment together with a gene for neomycin phosphotransferase II was stably introduced into tobacco using an Agrobacterium tumefaciens binary vector, and the fidelity of expression of the pea vicilin gene in its new host was studied. The seeds of eight transgenic tobacco plants showed a sixteen-fold range in the level of accumulated pea vicilin. The level of accumulation of vicilin protein and mRNA correlated with the number of integrated copies of the vicilin gene. Pea vicilin was confined to the seeds of transgenic tobacco. Using immunogold labelling, vicilin was detected in protein bodies of eight out of ten embryos (axes plus cotyledons) and, at a much lower level, in two out of eleven endosperms. Pea vicilin was synthesized early in tobacco seed development; some molecules were cleaved as is the case in pea seeds, yielding a major parental component of M r∼50000 together with a range of smaller polypeptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017468
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterisation of full-length cDNA clones of the giant taro (Alocasia macrorrhiza) trypsin/chymotrypsin inhibitor (1996)
    Springer
    Plant molecular biology 30 (1996), S. 1035-1039 
    Details
    ISSN: 1573-5028
    Keywords: giant taro ; proteinase inhibitor ; PCR ; RACE ; trypsin/chymotrypsin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA encoding the 206 amino acid open reading frame of a trypsin/chymotrypsin inhibitor abundant in the corms of giant taro (Alocasia macrorrhiza) was isolated. An internal fragment was cloned using degenerate primers corresponding to a region of the mature protein sequence and the ‘rapid amplification of cDNA ends’ (RACE) method used to generate a composite cDNA sequence. The length of the cDNA was close to the predicted size of the corresponding transcript deduced from northern blot analysis of corm mRNA. The inhibitor was expressed strongly in the mature corm, at low levels in leaf blades and petioles but not in roots. Southern blot analysis of the giant taro DNA indicated that this inhibitor is encoded by a small multigene family and this was further supported by the isolation of two different sequence classes from corm cDNA using primers to the ends of the composite sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020813
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Agrobacterium tumefaciens-gene transfer into wheat tissues (1991)
    Springer
    Plant cell, tissue and organ culture 25 (1991), S. 209-218 
    Details
    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; cocultivation ; tissue culture ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA can be transferred by Agrobacterium tumefaciens to wheat, albeit at very low frequencies. Transfer of agrobacterial DNA occurred in cultures where the embryos had been subjected to partial enzymatic digestion prior to cocultivation with the bacteria. It is unclear whether this is by the normal process mediated by the Ti virulence genes and the border repeats of the T-DNA. The Southern hybridization patterns indicate that in one cell line the T-DNA had undergone extensive rearrangements, and might indicate that the process of T-DNA transfer and integration might differ in the case of cereals. This could suggest the method of transfer and ultimately the expression of these genes in cereal cells may be different to that observed in other monocotyledonous and dicotyledonous species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036212
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    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Eucalyptus has a functional equivalent of the Arabidopsis floral meristem identity gene LEAFY (1998)
    Springer
    Plant molecular biology 37 (1998), S. 897-910 
    Details
    ISSN: 1573-5028
    Keywords: Eucalyptus globulus ; floral meristem identity gene ; flower development ; in situ hybridization ; LEAFY homologue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two genes cloned from Eucalyptus globulus, Eucalyptus LeaFy (ELF1 and ELF2), have sequence homology to the floral meristem identity genes LEAFY from Arabidopsis and FLORICAULA from Antirrhinum. ELF1 is expressed in the developing eucalypt floral organs in a pattern similar to LEAFY while ELF2 appears to be a pseudo gene. ELF1 is expressed strongly in the early floral primordium and then successively in the primordia of sepals, petals, stamens and carpels. It is also expressed in the leaf primordia and young leaves and adult and juvenile trees. The ELF1 promoter coupled to a GUS reporter gene directs expression in transgenic Arabidopsis in a temporal and tissue-specific pattern similar to an equivalent Arabidopsis LEAFY promoter construct. Strong expression is seen in young flower buds and then later in sepals and petals. No expression was seen in rosette leaves or roots of flowering plants or in any non-flowering plants grown under long days. Furthermore, ectopic expression of the ELF1 gene in transgenic Arabidopsis causes the premature conversion of shoots into flowers, as does an equivalent 35S-LFY construct. These data suggest that ELF1 plays a similar role to LFY in flower development and that the basic mechanisms involved in flower initiation and development in Eucalyptus are similar to those in Arabidopsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006056014079
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid (1993)
    Springer
    Transgenic research 2 (1993), S. 162-169 
    Details
    ISSN: 1573-9368
    Keywords: transgenic cotton plants ; 2,4-D herbicide tolerance ; herbicide detoxification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The agronomic performance of broad leaved crop plants such as cotton would be greatly improved if genetically-engineered resistance to broadleaf herbicides could both protect the plants from accidental spray drift damage and allow the suppression of problem broadleaf weeds by chemical means. Followingin vitro modification and the addition of plant expression signals, the gene for 2,4-D monooxygenase, a bacterial enzyme that degrades the broadleaf herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), was introduced into cotton plants byAgrobacterium-mediated transformation. First generation homozygous progeny of regenerated transgenic cotton plants carrying this gene exhibited up to a 50–100 fold increase in tolerance to 2,4-D compared with untransformed controls, and glasshouse trials suggest that the genetically-engineered plants would be completely protected from spray drift of 2,4-D, at least up to the normal field application rates commonly used on neighbouring cereal crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972610
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    Paper (German National Licenses)
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