GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Document type
Keywords
Publisher
Years
  • 1
    facet.materialart.
    Unknown
    H+-driven increase in CO2 uptake and decrease in HCO3- uptake explain coccolithophores' acclimation responses to ocean acidification (2016)
    AMER SOC LIMNOLOGY OCEANOGRAPHY
    In:  EPIC3Limnology and Oceanography, AMER SOC LIMNOLOGY OCEANOGRAPHY, 61, pp. 2045-2057, ISSN: 0024-3590
    Details
    Publication Date: 2016-11-17
    Description: Recent ocean acidification (OA) studies revealed that seawater [H+] rather than [CO2] or [HCO3-] regulate short-term responses in carbon fluxes of Emiliania huxleyi. Here, we investigated whether acclimation to altered carbonate chemistry modulates this regulation pattern and how the carbon supply for calcification is affected by carbonate chemistry. We acclimated E. huxleyi to present-day (ambient [CO2], [HCO3-], and pH) and OA conditions (high [CO2], ambient [HCO3-], low pH). To differentiate between the CO2 and pH/H+ effects, we also acclimated cells to carbonation (high [CO2] and [HCO3-], ambient pH) and acidification (ambient [CO2], low [HCO3-], and pH). Under these conditions, growth, production of particulate inorganic and organic carbon, as well as carbon and oxygen fluxes were measured. Under carbonation, photosynthesis and calcification were stimulated due to additional inline image uptake, whereas growth was unaffected. Such stimulatory effects are not apparent after short-term carbonation, indicating that cells adjusted their carbon acquisition during acclimation. Being driven by H+, these regulations can, however, not explain typical OA effects. Under acidification and OA, photosynthesis stayed constant, whereas calcification and growth decreased. Similar to the short-term responses toward high [H+], CO2 uptake significantly increased, but inline image uptake decreased. This antagonistic regulation in CO2 and inline image uptake can explain why photosynthesis, being able to use CO2 and inline image, often benefits from OA, whereas calcification, being mostly dependent on inline image, often decreases. We identified H+ as prime driver of coccolithophores' acclimation responses toward OA. Acidified conditions seem to put metabolic burdens on the cells that result in decreased growth.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 2
    facet.materialart.
    Unknown
    Carbonation versus acidification effects on cellular carbon fluxes of Emiliania huxeleyi (2016)
    In:  EPIC3The IXth International Symposium on Inorganic Carbon Utilization by Aquatic Photosynthetic Organisms (CCM9), Clare College, Cambridge, UK, 2016-08-15-2016-08-17
    Details
    Publication Date: 2017-01-10
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 3
    facet.materialart.
    Unknown
    Acidification, not carbonation, is the major regulator of carbon fluxes in the coccolithophore Emiliania huxleyi (2016)
    WILEY-BLACKWELL PUBLISHING
    In:  EPIC3New Phytologist, WILEY-BLACKWELL PUBLISHING, 211, pp. 126-137, ISSN: 0028-646X
    Details
    Publication Date: 2016-11-16
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects (2014)
    SPRINGER
    In:  EPIC3Photosynthesis Research, SPRINGER, 121, pp. 265-275, ISSN: 0166-8595
    Details
    Publication Date: 2016-02-23
    Description: Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 μatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a 14C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9–8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 − uptake depended strongly on the assay pH. At pH values ≤ 8.1, cells preferentially used CO2 (≥ 90 % CO2), whereas at pH values ≥ 8.3, cells progressively increased the fraction of HCO3 − uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the 14C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 − usage seen in previous studies.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev , info:eu-repo/semantics/article
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 5
    facet.materialart.
    Unknown
    Process understanding of photosynthetic fluxes underlying ocean acidification responses in the coccolithophore Emiliania huxleyi (2016)
    Universität Bremen
    In:  EPIC3Universität Bremen, 103 p.
    Details
    Publication Date: 2016-05-12
    Repository Name: EPIC Alfred Wegener Institut
    Type: Thesis , notRev
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 6
    facet.materialart.
    Unknown
    Diel variations in cell division and biomass production of Emiliania huxleyi -- Consequences for the calculation of physiological cell parameters (2020)
    ASLO
    In:  EPIC3Limnol. Oceanogr., ASLO, pp. 1-20
    Details
    Publication Date: 2020-03-26
    Description: Cell division of the coccolithophore Emiliania huxleyi and other phytoplankton typically becomes entrained to diel light/dark cycles under laboratory conditions, with division occurring primarily during dark phases and production occurring during light phases. Under these conditions, increases in cell and biomass concentrations deviate from exponential functions on time scales 〈 24 h. These deviations lead to significant diel variations in common measurements of phytoplankton physiology such as cellular quotas of particulate organic and inorganic carbon (POC, PIC) and their production rates. Being time-dependent, only the temporal mean of the various values during the day are comparable between experiments. Deviations from exponential growth furthermore imply that increases in cell and biomass concentrations cannot be expressed by the daily growth rate μ_{24 h} (typically determined from daily increments in cell concentrations). Consequently, conventional calculations of production as the product of a cellular quota (e.g., POC quota) and μ_{24 h} are mathematically incorrect. To account for this, we here describe short-term changes in cell and biomass concentrations of fastdividing, dilute-batch cultures of E. huxleyi grown under a diel light/dark cycle using linear regression. Based on the derived models, we present calculations for daily means of cellular quotas and production rates. Conventional (time-specific) measurements of cellular quotas and production differ from daily means by up to 65% in our example and, under some circumstances, cause false “effects” of treatments. Intending to reduce errors in ecophysiological studies, we recommend determining daily means—mathematically or by adjusting the experimental setup or sampling times appropriately.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , isiRev
    Format: application/pdf
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    PAPER (OA)
  • 7
    facet.materialart.
    Unknown
    Culture conditions, mass spectrometric measurements and acclimation carbonate chemistry (2016)
    PANGAEA
    In:  Supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Rost, Björn (2016): Acidification, not carbonation, is the major regulator of carbon fluxes in the coccolithophore Emiliania huxleyi. New Phytologist, 211(1), 126-137, https://doi.org/10.1111/nph.13885
    Details
    Publication Date: 2023-03-16
    Description: - A combined increase in seawater [CO2] and [H+] was recently shown to induce a shift from photosynthetic HCO3- to CO2 uptake in Emiliania huxleyi. This shift occurred within minutes, whereas acclimation to ocean acidification (OA) did not affect the carbon source. - To identify the driver of this shift, we exposed low- and high-light acclimated E. huxleyi to a matrix of two levels of dissolved inorganic carbon (1400, 2800 lmol kg-1) and pH (8.15, 7.85) and directly measured cellular O2, CO2 and HCO3 fluxes under these conditions. - Exposure to increased [CO2] had little effect on the photosynthetic fluxes, whereas increased [H+] led to a significant decline in HCO3- uptake. Low-light acclimated cells overcompensated for the inhibition of HCO3- uptake by increasing CO2 uptake. High-light acclimated cells, relying on higher proportions of HCO3- uptake, could not increase CO2 uptake and photosynthetic O2 evolution consequently became carbon-limited. - These regulations indicate that OA responses in photosynthesis are caused by [H+] rather than by [CO2]. The impaired HCO3- uptake also provides a mechanistic explanation for lowered calcification under OA. Moreover, it explains the OA-dependent decrease in photosynthesis observed in high-light grown phytoplankton.
    Keywords: AWI_Phytochange; Phytochange @ AWI
    Type: Dataset
    Format: application/zip, 16.7 kBytes
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 8
    facet.materialart.
    Unknown
    Acidification, not carbonation, is the major regulator of carbon fluxes in the coccolithophore Emiliania huxleyi (2016)
    PANGAEA
    Details
    Publication Date: 2024-05-22
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bicarbonate uptake rate; Bicarbonate uptake rate, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon dioxide uptake, standard deviation; Carbon dioxide uptake rate; Carbon dioxide usage fraction; Carbon dioxide usage fraction, standard deviation; Chromista; Colorimetric; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth rate; Growth rate, standard deviation; Haptophyta; Hydrogen ion concentration; Hydrogen ion concentration, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Mass spectrometry; Net photosynthesis rate; Net photosynthesis rate, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Potentiometric titration; Pressure, water; Primary production/Photosynthesis; Registration number of species; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Silicate; Single species; Species; Temperature, water; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1710 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 9
    facet.materialart.
    Unknown
    Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects (2014)
    PANGAEA
    In:  Supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Björn (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275, https://doi.org/10.1007/s11120-014-9984-9
    Details
    Publication Date: 2024-05-27
    Description: Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 µatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a (14)C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9-8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 (-) uptake depended strongly on the assay pH. At pH values =〈 8.1, cells preferentially used CO2 (〉= 90 % CO2), whereas at pH values 〉= 8.3, cells progressively increased the fraction of HCO3 (-) uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the (14)C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 (-) usage seen in previous studies.
    Keywords: Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate, per cell; Carbon, total, particulate, per cell; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide usage fraction; Chlorophyll a per cell; Chromista; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Haptophyta; Irradiance; Laboratory experiment; Laboratory strains; Light:Dark cycle; Nitrogen, organic, particulate, per cell; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Phosphate; Phytoplankton; Potentiometric; Potentiometric titration; Pressure, water; Salinity; Silicate; Single species; Species; Strain; Temperature, water; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 548 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 10
    facet.materialart.
    Unknown
    Results of a 24-hour laboratory experiment sampling cell concentrations, particulate organic carbon (POC), particulate inorganic carbon (PIC) and further cell parameters of the coccolithophore Emiliania huxleyi grown under a 16:8 h light/dark cycle (2020)
    PANGAEA
    Details
    Publication Date: 2024-05-27
    Description: Cell division of the coccolithophore Emiliania huxleyi and other phytoplankton typically becomes entrained to diel light/dark cycles under laboratory conditions, with division occurring primarily during dark phases and production occurring during light phases. Under these conditions, the increase in a culture's cell and biomass concentrations deviates from an exponential function on time scales 〈 24 h. We here present a dataset of short-term changes in cell and biomass concentrations of fast dividing, dilute-batch cultures of E. huxleyi grown under a 16:8 h light/dark cycle. This dataset was used to derive linear models describing the diel course in the concentrations of cells, particulate organic carbon (POC) and particulate inorganic carbon (PIC) and for the calculation of daily means of cellular quotas and production rates in Kottmeier et al. (2020). We also present the given seawater carbonate chemistry as well as cellular quotas of particulate organic nitrogen (PON) and chlorophyll a (Chl. a), and the ratios of PIC:POC, POC:PON, POC:cell volume and Chl. a:POC in the course of the 24 h sampling period.
    Keywords: 1; Alkalinity, potentiometric; Alkalinity, total; Alkalinity, total, standard deviation; Calculated using CO2SYS; Carbon, inorganic, dissolved; Carbon, inorganic, particulate, per cell; Carbon, inorganic, particulate, relative concentration; Carbon, organic, particulate; Carbon, organic, particulate, per cell; Carbon, organic, particulate, relative concentration; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Cell concentration, relative; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Colorimetric autoanalysis; Consumption of carbon, inorganic, dissolved, standard deviation; Coulter counter, Beckman Coulter, Multisizer 3; Elemental analyzer, EuroVector, EA 3000; Exponential growth; Fluorometer, Turner Design, TD-700; Growth rate; Growth rate, standard deviation; Hand net; HN; Identification; Irradiance; Light/dark cycles; Light meter; LM; Nitrogen, organic, particulate, per cell; Particulate inorganic carbon/particulate organic carbon ratio; pH; pH, standard deviation; Phase; Phased cell division; PIC production; POC production; Potentiometric; Registration number of species; Salinity; SALINO; Salinometer; SO136; SO136_006-A_HPN; Sonne; Species; Stage; Strain; TASQWA; Temperature, water; Temperature sensor; Time in hours; Treatment: light:dark cycle; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1983 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...