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  • 1
    Electronic Resource
    Electronic Resource
    Preliminary studies on the isolation of coronavirus 229E nucleocapsids (1979)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 5 (1979), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1979.tb03256.x
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  • 2
    Electronic Resource
    Electronic Resource
    An engineered poliovirus chimaera elicits broadly reactive HIV-1 neutralizing antibodies (1989)
    [s.l.] : Nature Publishing Group
    Nature 339 (1989), S. 385-388 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We have used recombinant DNA techniques to introduce antigenic domains from the capsid proteins of poliovirus type 3 into poliovirus type 1 to produce a virus chimaera of dual antigenicity and immunogenicity1. These and related observations2'3 encouraged us to develop the Sabin type 1 vaccine ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/339385a0
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  • 3
    Electronic Resource
    Electronic Resource
    Antigen chimaeras of poliovirus as potential new vaccines (1988)
    [s.l.] : Nature Publishing Group
    Nature 332 (1988), S. 81-82 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The live attenuated poliovirus vaccines in current use are derived from strains developed by multiple passage of wild-type viruses in non-human primates and in tissue culture8. Although the use of these vaccines has almost eliminated poliomyelitis in developed countries, both type 2 and type 3 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/332081a0
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  • 4
    Electronic Resource
    Electronic Resource
    SSPE measles virus: Non-productive and productive infection in vero cells and suckling mice (1974)
    Springer
    Medical microbiology and immunology 160 (1974), S. 201-208 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Suckling mice have proved successful for the isolation of measles virus from the brain biopsy specimens of two clinical eases of subacute sclerosing panencephalitis (SSPE). A productive infection, with cell-free virus, has been obtained in mouse brain tissues with both isolates. Subsequent infection of Vero cell cultures was obtained with mouse brain homogenates but not with mouse brain derived cell-free virus, thereby mimicking the situation with SSPE in which cocultivation of brain and Vero cells is needed to infect the Vero cells. A non-productive infection, with cell-associated virus, has been obtained in Vero cells for a limited period with one isolate (LW) and for an indefinite period with the other isolate (MD). Infectivity experiments with MD isolate suggest that the cell-associated character of this SSPE measles virus is not an indication of genomic defectiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02121727
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  • 5
    Electronic Resource
    Electronic Resource
    Population genetic structure in Lens taxa revealed by isozyme and RAPD analysis (1998)
    Springer
    Genetic resources and crop evolution 45 (1998), S. 549-559 
    Details
    ISSN: 1573-5109
    Keywords: AMOVA ; Lens ; population genetic structure ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An understanding of the genetic structure of populations is vital for the formation of optimum collection, conservation and utilization strategies for plant genetic resources. This is of particular importance in the case of in-situ conservation, a strategy gaining in popularity. The population genetic structures of five wild lentil taxa, Lens culinaris subsp. orientalis, L. odemensis, L. ervoides, L. nigricans and L. lamottei were investigated using isozyme electrophoresis and random amplified polymorphic DNA (RAPD). Approximately 20 plants from each of 5 populations per taxon were screened for variation at 11 isozyme loci and using three RAPD primers. Levels of variation were generally low, although considerable variation existed in the levels of diversity found within populations of L. culinaris subsp. orientalis and L. lamottei. Comparison of the results obtained in this study with the results obtained in a previous study indicate that this is a trend occurring across all species. It implies that levels of diversity within populations must be measured and considered prior to targeting of specific populations for in-situ conservation. Analysis of molecular variance of both isozyme and RAPD data revealed that between 78% and 99% of the variation was attributable to between-population differences. Isozyme results from L. lamottei populations were, however, contradictory. Possible explanations for this difference are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008640201896
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological and phenological variation in the wild relatives of lentil (1999)
    Springer
    Genetic resources and crop evolution 46 (1999), S. 3-12 
    Details
    ISSN: 1573-5109
    Keywords: agro-morphology ; geographical distribution ; Lens ; phenology ; wild relatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wild lentils are a potentially important source of genetic variation for the improvement of the cultivated lentil. A lack of evaluation data for characters of economic importance is one constraint to their use in breeding programmes. Here, variation in selected phenological and agro-morphological characters in 310 accessions of wild lentils is reported. This includes 153 accessions of Lens culinaris subsp. orientalis, 33 accessions of L. odemensis, 32 accessions of L. nigricans, 90 accessions of L. ervoides and 2 accessions of L. lamottei. Certain L. culinaris subsp. orientalis accessions had substantially more leaves per plant, peduncles per plant, pods per plant and seeds per plant, and greater leaf area than two cultivated lentil checks. The total biomass obtained from the best L. culinaris subsp. orientalis accessions was comparable with the checks. The harvest index of one check was comparable with that of the two best L. culinaris subsp. orientalis accessions. Of the wild taxa, the L. lamottei had the highest average 100-seed weight. Broad-sense heritabilities were calculated and found to be high for days to average flowering and days to average podding. Significant correlations exist between quantitative characters and latitude of origin. Phenological adaptation, through sensitivity to photoperiod, temperature or both, appear to be a major evolutionary force in wild lentils. Variation is mapped according to geographical origin of accessions in order to identify geographical patterns or clines of variation. Accessions of L. culinaris subsp. orientalis from Uzbekistan, Turkmenistan and Tajikistan had among the largest biomass, the most peduncles per plant and many pods and seeds per plant. One-hundred seed weight, however, did not decline as expected with increased seed number.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008645029658
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  • 7
    Electronic Resource
    Electronic Resource
    Contrasting genetic variation amongst lentil landraces from different geographical origins (1998)
    Springer
    Euphytica 102 (1998), S. 265-273 
    Details
    ISSN: 1573-5060
    Keywords: bottleneck ; isozyme ; landrace ; lentil ; RAPD ; South Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lentil landraces from South Asia exhibit a low diversity and discordance with landraces from other countries according to a combination of qualitative and quantitative agromorphological characters. They exhibit specific phenological adaptation to the South Asian environment which precludes the direct use of alien germplasm in breeding programs in South Asia. An understanding of the genetic relationships and diversity of South Asian lentil landraces, in relation to landraces from other countries, is important in attempting to widen the genetic base of germplasm in the region. The objectives of this study were to investigate the genetic relationships between lentil landraces from 3 South Asian countries (India, Nepal and Pakistan) and those from 13 other countries and to determine their relative genetic diversities, using both isozyme electrophoresis and random amplified polymorphic DNA (RAPD) analysis. Polymorphisms were observed for 7 isozyme loci (16 alleles) and 22 RAPD loci. According to Nei's genetic distance, germplasm from Afghanistan clustered with that from the South Asian countries. The germplasm from these countries was striking different to that from the other countries studied. Based on genetic distance estimates from RAPD analysis, the countries with the lowest diversity were Pakistan, Afghanistan and Nepal. These data support evidence at the morphological level of a genetic bottleneck in lentil landraces from South Asia. Genetic relationships between countries outside the South Asian group are discussed. Classification into macrosperma and microsperma types did not reflect overall country relationships.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018331432580
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  • 8
    Electronic Resource
    Electronic Resource
    Genetic diversity and taxonomic relationships within the genus Lens as revealed by allozyme polymorphism (1996)
    Springer
    Euphytica 91 (1996), S. 163-172 
    Details
    ISSN: 1573-5060
    Keywords: genetic distance ; genetic diversity ; allozyme ; Lens ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A survey of allozyme polymorphism at 11 loci was carried out on 439 accessions from the genus Lens. This comprised 153 Lens culinaris subsp. orientalis, 35 L. odemensis, 117 L. ervoides, 32 L. nigricans, 2 of a differentiated cytotype of L. nigricans and 100 landrace accessions of the cultivated lentil (L. culinaris subsp. culinaris), from 10 different countries. The aim of the survey was to determine intra-specific genetic diversity and species relationships, based on phylogenetic and phenetic analyses, particularly regarding the position of L. odemensis and the differentiated cytotype of L. nigricans. Diversity was described by three statistics. The level of diversity in the cultivated taxon was lower than in any of the wild species according to two of these statistics, the percentage of polymorphic loci and mean number of alleles per locus. For the third measure (Nei's mean genetic diversity) it was only greater than L. ervoides. Genetic diversity statistics of the wild species indicated differences in the nature of between-population genetic diversity within the different taxa. Phylogenetic analysis suggests that L. odemensis and L. ervoides evolved from a common ancestor, and L. culinaris subsp. orientalis subsequently evolved from L. odemensis. Phenetic analysis, however, places L. odemensis closer to L. culinaris subsp. orientalis than to L. ervoides. Nei's mean genetic distance of L. odemensis from both L. culinaris subsp. culinaris (0.204) and L. culinaris subsp. orientalis (0.110) was greater than the distance between them (0.062). This evidence is not conclusive in determining whether L. odemensis should retain its specific status. Further crossability studies should be carried out on a range of genotypes to assess the potential for gene flow. The evidence presented shows the differentiated cytotype of L. nigricans to be quite distinct from other L. nigricans accessions, both phenetically and phylogenetically. This indicates that the differentiated cytotype of L. nigricans may constitute a new taxon. Discriminant function analysis reveals that isozymes may be useful in validating species classification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021066
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