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  • 1
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Salmonella ushB, which encodes a membrane-bound UDP-sugar hydrolase, has an Escherichia coli orthologue (ushBc) which does not detectably produce this activity. In this report, we show that ushBc does not produce any detectable protein either, despite being transcribed normally. Remarkably, ushBc is shown to have 100% sequence identity with E. coli cdh, previously characterised as encoding an active CDP-diglyceride hydrolase, an apparent contradiction with implications regarding enzyme evolution. We suggest that a useful gene designation is cdh (ushBc) rather than either ushBc or cdh, alone.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Escherichia coli contains a single periplasmic UDP-glucose hydrolase (5′-nucleotidase) encoded by ushA. Salmonella enterica, serotype Typhimurium, also contains a single UDP-glucose hydrolase but, in contrast to E. coli, it is membrane-bound and is encoded by the non-homologous ushB gene; Salmonella enterica (Typhimurium) also contains a silent allele of the ushA gene (ushA0). In this report, we show that nearly all natural isolates of Salmonella contain both UDP-sugar hydrolases, i.e. they are UshA+ UshB+. The only exceptions are all from sub-group I (S. gallinarum, S. pullorum, and most Typhimurium strains), are UshA− UshB+, and several have been shown to contain an ushA0 allele. These data, together with the fact that these latter strains are closely related genetically, strongly suggests a recent silencing mutation(s). We also report the presence in E. coli K-12, and in natural isolates of E. coli, of a DNA sequence which is homologous to the ushB gene of Salmonella; since E. coli does not contain UshB activity, we tentatively refer to this sequence as ushB0. Since all E. coli strains investigated are UshB−, we conclude that the silencing mutation(s) occured relatively eary following the divergence of Escherichia coli and Salmonella from a common ancestor that was ushA+ ushB+.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cancer chemotherapy and pharmacology 20 (1987), S. 162-168 
    ISSN: 1432-0843
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The nuclear enzyme, topoisomerase II, is the major site of action for cancer chemotherapy agents such as etoposide, teniposide, and a variety of intercalating agents. These compounds cause the enzyme to cleave DNA, forming a DNA-protein complex that may be a key step leading to cell death. It is apparently unique as a chemotherapy target, since drug potency diminishes with decreasing enzyme activity. It was thus of interest to examine the topoisomerase content and drug-induced DNA cleavage in freshly obtained human leukemia cells and to compare the obtained data with the results of similar studies performed in well-characterized human leukemia cell lines. The human T-lymphoblast line, CCRF-CEM, was more than 100-fold more sensitive to the DNA-cleavage effect of etoposide than the cells of the 13 leukemic patients examined. One of the leukemia lines (HL-60) and a lymphoblastoid line (RPMI-7666) were somewhat less sensitive than cells of the CCRF-CEM cells, but were still 10-fold more sensitive than the patients studied. The relative insensitivity of the freshly obtained cells could not be accounted for by differences with respect to drug uptake but were associated with markedly reduced topoisomerase-II content as assayed by immunoblotting using a mouse polyclonal serum against topoisomerase II. Heterogeneity was observed in the sensitivities of patients' cells with respect to both drug-induced DNA cleavage and enzyme content. The observed differences between cultured cell lines and patients' cells may have been related to their proliferative status. Etoposide potency in normal resting lymphocytes resembles that observed in circulating leukemia cells. However, following mitogenesis with phytohemagglutinin and interleukin-2, proliferating lymphocytes become as sensitive to etoposide as cultured cell lines with regard to DNA cleavage. This effect was accompanied by an increase in topoisomerase-II content. Our data thus support the hypothesis that topoisomerase-II content may be an important determinant of cell sensitivity to certain classes of chemotherapy agents. Efforts to stimulate topoisomerase-II content may improve the therapeutic efficacy of these drugs.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Islet amyloid peptide ; Pancreatic islets ; Type-2 diabetes ; Insulin ; Lysosomes ; Secretory granules ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Islet amyloid peptide (or diabetes-associated peptide), the major component of pancreatic islet amyloid found in type-2 diabetes, has been identified by electronmicroscopic immunocytochemistry in pancreatic B-cells from five non-diabetic human subjects, and in islets from five type-2 diabetic patients. The greatest density of immunoreactivity for islet amyloid peptide was found in electrondense regions of some lysosomal or lipofuscin bodies. The peptide was also localised by quantification of immunogold in the secretory granules of B-cells, and was present in cytoplasmic lamellar bodies. Acid phosphatase activity was also demonstrated in these organelles. Immunoreactivity for insulin was found in some lysosomes. These results suggest that islet amyloid peptide is a constituent of normal pancreatic B-cells, and accumulates in lipofuscin bodies where it is presumably partially degraded. In islets from type-2 diabetic subjects, amyloid fibrils and lipofuscin bodies in B-cells showed immunoreactivity for the amyloid peptide. Abnormal processing of the peptide within B-cells could lead to the formation of islet amyloid in type-2 diabetes.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2022-05-26
    Description: Dataset: LB_2012_Ship_Chl
    Description: Chlorophyll and pheopigments from filtered water samples from R/V Savannah cruises in the South Atlantic Bight (SAB) continental shelf off Long Bay, January-April 2012. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/638203
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1032285, NSF Division of Ocean Sciences (NSF OCE) OCE-1032276
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 6
    Publication Date: 2022-08-25
    Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Seim, H., Savidge, D., Andres, M., Bane, J., Edwards, C., Gawarkiewicz, G., He, R., Todd, R., Muglia, M., Zambon, J., Han, L., & Mao, S. Overview of the Processes driving Exchange at Cape Hatteras Program. Oceanography, (2022), https://doi.org/10.5670/oceanog.2022.205.
    Description: The Processes driving Exchange At Cape Hatteras (PEACH) program seeks to better understand seawater exchanges between the continental shelf and the open ocean near Cape Hatteras, North Carolina. This location is where the Gulf Stream transitions from a boundary-trapped current to a free jet, and where robust along-shelf convergence brings cool, relatively fresh Middle Atlantic Bight and warm, salty South Atlantic Bight shelf waters together, forming an important and dynamic biogeographic boundary. The magnitude of this convergence implies large export of shelf water to the open ocean here. Background on the oceanography of the region provides motivation for the study and gives context for the measurements that were made. Science questions focus on the roles that wind forcing, Gulf Stream forcing, and lateral density gradients play in driving exchange. PEACH observational efforts include a variety of fixed and mobile observing platforms, and PEACH modeling included two different resolutions and data assimilation schemes. Findings to date on mean circulation, the nature of export from the southern Middle Atlantic Bight shelf, Gulf Stream variability, and position variability of the Hatteras Front are summarized, together with a look ahead to forthcoming analyses.
    Description: We gratefully acknowledge NSF funding (OCE-1558920 to UNC-CH, OCE-1559476 to SkIO, OCE-1558521 to WHOI, OCE-1559178 to NCSU); technical support from Sara Haines, Craig Marquette, Trip Patterson, Nick DeSimone, Erran Sousa, Gabe Matthias, Patrick Deane, Brian Hogue, Frank Bahr, and Ben Hefner; cruise participants Jacob Forsyth, Joleen Heiderich, Chuxuan Li, Marco Valero, Lauren Ball, John McCord, and Kyle Maddux-Lawrence; and the crew of R/V Armstrong for their able support during three PEACH cruises.
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 7
    Publication Date: 2022-05-26
    Description: Dataset: LB_2012_Glider_DO_L2
    Description: Two Webb Slocum gliders were used to measure salinity, temperature, and depth. Glider 'Pelagia' was deployed in Long Bay, S. Carolina, South Atlantic Bight and glider 'Ramses' was deployed along the upper slope of S. Carolina. in 2012. Data were collected in a time series from January to April, 2012. NOTE: This is a very large dataset (1.53 million records). It takes a while to serve and may not display entirely depending on your browser. You can download all the data from the Data Files section on this metadata page. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/636636
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1032285, NSF Division of Ocean Sciences (NSF OCE) OCE-1032276
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 8
    Publication Date: 2022-05-26
    Description: Dataset: LB_2012_Mooring_Optics
    Description: Time series of chlorophyll and turbidity measured every second on three moorings located at Long Bay, S. Carolina in the South Atlantic Bight, located at mid-shelf at 30 m, shelf break at 74 m and upper slope at 171 m along a central shelf/slope survey line SE of Myrtle Beach, SC between 33.17/-78.33 and 32.76/-77.91. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/638349
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1032285, NSF Division of Ocean Sciences (NSF OCE) OCE-1032276
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 9
    Publication Date: 2022-05-26
    Description: Dataset: LB_2012_Glider_CTD
    Description: Two Webb Slocum gliders were used to measure salinity, temperature, and depth. Glider 'Pelagia' was deployed in Long Bay, S. Carolina, South Atlantic Bight and glider 'Ramses' was deployed along the upper slope of S. Carolina. in 2012. Data were collected in a time series from January to April, 2012. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/544615
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1032285, NSF Division of Ocean Sciences (NSF OCE) OCE-1032276
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 10
    Publication Date: 2022-05-26
    Description: Dataset: LB_2012_Ship_Nutrients
    Description: Long Bay ship macronutrient concentrations from R/V Savannah (SAV-12-03, SAV-12-05, SAV-12-11) cruises in the South Atlantic Bight (SAB) continental shelf off Long Bay during 2012. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/638156
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1032285, NSF Division of Ocean Sciences (NSF OCE) OCE-1032276
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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