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  • 1
    Electronic Resource
    Electronic Resource
    Circular Ribosomal RNA Genes Are a General Feature of Schizopyrenid Amoebae (1988)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 35 (1988), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have recently shown that the ribosomal RNA genes of the amoebo-flagellate Naegleria gruberi Schardinger, 1899, strain NEG-M are carried exclusively on a 14 kilobasepair plasmid. To explore the distribution of this unique gene arrangement, we have examined another strain of N. gruberi and four other species from the order Schizopyrenida. All have this unusual gene arrangement although the size of the plasmid varies widely. Species groups based on morphological criteria do not agree with those resulting from comparison of plasmid restriction enzyme patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1988.tb04352.x
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  • 2
    Electronic Resource
    Electronic Resource
    Electrophoretic Karyotype and Linkage Groups of the Amoeboflagellate Naegleria gruberi (1990)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 37 (1990), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . We have constructed a molecular karyotype for two strains of Naegleria gruberi using pulsed field gel electrophoresis. Each strain has about 23 chromosomes, considerably more than any previous estimate. These chromosomes range in size from 400 kilobasepairs to over 2,000 kilobasepairs. In Naegleria, construction of the DNA karyotype depends on assessment of the anomalous electrophoretic mobility of the circular ribosomal RNA genes. We have determined the chromosomal locations of an identified unique gene (flagellar calmodulin) and four identified multigene families (α- and β-tubulin, actin, ubiquitin), as well as three differentially expressed genes of unknown functions. The ca. 12 actin genes are dispersed over at least seven chromosomes, whereas the majority of the more than eight α-tubulin genes are confined to a single chromosome. The ubiquitin genes are found on five chromosomes in one strain and seven in the other and the β-tubulin genes are on three or four. Our observations provide a foundation for molecular genetic studies in this organism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1990.tb01164.x
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning of foot and mouth disease virus genome and nucleotide sequences in the structural protein genes (1981)
    [s.l.] : Nature Publishing Group
    Nature 290 (1981), S. 800-802 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Using two strains of FMDV (serotypes A10, strain 61 and O1, strain BFS), double-stranded (ds) cDNA was synthesized8 and recombinant clones derived as described elsewhere9. Briefly, FMDV RNA (10 µg), primed with oligo (dT)12-18, was used as a template for ds cDNA synthesis by reverse ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/290800a0
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  • 4
    Electronic Resource
    Electronic Resource
    Crossreacting determinants in the C-terminal region of trypanosome variant surface antigens (1979)
    [s.l.] : Nature Publishing Group
    Nature 277 (1979), S. 310-312 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Immunoprecipitation of four VSGs and three N-terminal tryptic fragments by homologous and heterologous antisera. The antigens are VSGs 55 (a); 221 (b}\ 121 (c), 116 (d}\ and N-terminal fragments from VSGs 55 (e}\ 221 (/); and 121 (g). O, Homologous antisera; , heterologous antisera. Antigens ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/277310a0
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  • 5
    Electronic Resource
    Electronic Resource
    Protective monoclonal antibodies recognising stage-specific merozoite antigens of a rodent malaria parasite (1980)
    [s.l.] : Nature Publishing Group
    Nature 284 (1980), S. 366-368 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Spleen cells from two P. yoelii-immune BALB/c mice were fused with P3-NSl/l-Ag4-l myeloma cells in the presence of polyethylene glycol7. The cells were dispensed into 144 tissue culture wells in 2-ml volumes of hypoxanthine-aminopterine-thymidine-selective medium8, using RPMI-1640 medium ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/284366a0
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  • 6
    Electronic Resource
    Electronic Resource
    N-terminal amino acid sequences of variant-specific surface antigens from Trypanosoma brucei (1976)
    [s.l.] : Nature Publishing Group
    Nature 263 (1976), S. 613-614 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Amino terminal sequences of variant-specific surface antigens from four successive trypanosome variants. Sequences were obtained by automated liquid-phase and solid-phase techniques (see text). Phenylthiohydantoin derivatives were identified by high-pressure liquid chromatography on a column ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/263613a0
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  • 7
    Electronic Resource
    Electronic Resource
    Primary structure of the histidine-rich protein of Plasmodium lophurae (1986)
    [s.l.] : Nature Publishing Group
    Nature 324 (1986), S. 492-492 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-We wish to address the literature concerning the sequence of the histidine-rich protein (HRP) and the corresponding gene from the avian malaria parasite Plasmodium lophurae. Following publication of a DNA sequence derived from a P. lophurae genomic clone1, we were forced to reassess our own ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/324492b0
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  • 8
    Electronic Resource
    Electronic Resource
    Release and purification of trypanosoma brucei variant surface glycoprotein (1984)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 24 (1984), S. 79-90 
    Details
    ISSN: 0730-2312
    Keywords: Trypanosoma brucei ; variant surface glycoprotein ; purification ; release ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Conditions affecting the solubilization of variant surface glycoprotein (VSG) from Trypanosoma brucei have been investigated. The results obtained form the basis for a convenient and efficient method for VSG purification. VSG release from the cell surface was temperature-dependent, following osmotic lysis at 0 °C, and was inhibited by low concentrations of Zn2+ but not by tosyl-lysine chloromethyl-ketone (TLCK), phenylmethylsulfonylfluoride (PMSF), or iodoacetamide. These and other results eliminated the possibility that release was due to proteolytic cleavage of the C-terminal hydrophobic tail present on newly synthesized VSG. Bolton and Hunter reagent reacted with several components on living cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240240107
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  • 9
    Electronic Resource
    Electronic Resource
    Antigenic variation in trypansosomes: Secrets surface slowly (1996)
    New York, NY : Wiley-Blackwell
    BioEssays 18 (1996), S. 283-291 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Among pathogenic micro-organisms that evade the mammalian immune responses, Trypanosoma brucei has developed the most elaborate capacity for antigenic variation. Trypanosomes branched early during eukaryotic evolution. They are characterized by many aberrations, ranging from the unusual compartmentation of metabolic pathways to the heresy of RNA editing. The ubiquitous phenomenon of glycosylphosphatidylinositol-anchoring of eukaryotic plasma membrane proteins and RNA trans-splicing (trypanosome genes contain no introns), which adds an identical leader sequence to all trypanosome mRNAs, were first defined during studies of antigenic variation. Genetic transformation of trypanosomes and the high efficiency of gene targeting provide new opportunities to investigate the regulation of antigenic variation. There is every reason to expect trypanosomes to provide further surprises and insights into the evolution of genetic regulatory mechanisms.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950180406
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