Description: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the detection and quantitation of karlotoxins in the selected reaction monitoring (SRM) mode. This novel method was based upon the analysis of purified karlotoxins (KcTx-1, KmTx-2, 44-oxo-KmTx-2, KmTx-5), one amphidinol (AM-18), and unpurified extracts of bulk cultures of the marine dinoflagellate Karlodinium veneficum strain CCMP2936 from Delaware (Eastern USA), which produces KmTx-1 and KmTx-3. The limit of detection of the SRM method for KmTx-2 was determined as 2.5 ng on-column. Collision induced dissociation (CID) spectra of all putative karlotoxins were recorded to present fragmentation patterns of each compound for their unambiguous identification. Bulk cultures of K. veneficum strain K10 isolated from an embayment of the Ebro Delta, NW Mediterranean, yielded five previously unreported putative karlotoxins with molecular masses 1280, 1298, 1332, 1356, and 1400 Da, and similar fragments to KmTx-5. Analysis of several isolates of K. veneficum from the Ebro Delta revealed small-scale diversity in the karlotoxin spectrum in that one isolate from Fangar Bay produced KmTx-5, whereas the five putative novel karlotoxins were found among several isolates from nearby, but hydrographically distinct Alfacs Bay. Application of this LC-MS/MS method represents an incremental advance in the determination of putative karlotoxins, particularly in the absence of a complete spectrum of purified analytical standards of known specific potency.

Description: We present the test of a simple and rapid methodology for earthquake detection and phase association dedicated to dense and reliable networks of seismic stations. The system is ba- sed on the idea of small subnets of stations each one surrounding the station that produce the first trigger. The goal is to provide a rapid detection of relevant earthquakes and to activate ElarmS but also to prevent misinterpretation of out of the network events or the association of false triggers. We apply this system to the case of the Italian National Seismic Network and to the Italian seismicity.

Description: Unpublished

Description: Creete, Greece

Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale

Description: open

Description: The new Italian National Seismic Network (INSN) is a dense network of broadband stations deployed for monitoring Italian seismicity. The network consists of 250 stations with a typical station spacing of !40 km. Earthquake early warning is the rapid detection of an event in progress, assessment of the hazard it poses, and transmission of a warning ahead of any significant ground motion. We explore the potential for using the INSN real-time network for the purpose of earthquake early warning. We run the ElarmS early warning methodology off-line using a data set of more than 200 events with magnitudes between 2.5 and 6.0. A scaling relation for magnitude determination from the dominant period of the first seconds of signal following the P onset is developed from the data set. The standard deviation in the magnitude estimates using this approach is 0.4 magnitude units, and all event magnitude estimates are within !0:75 magnitude units of the true magnitude. Given the existing distribution of seismic stations it takes an average of 10 sec after event initiation before the P wave has been detected at four stations. If we require a detection at four stations before issuing the first alert, then the blind zone, within which no warning would be available, has a radius of !37 km. The ElarmS methodology can provide a warning earlier than this but with a greater uncertainty. An assessment of past damaging earthquakes across Italy shows that applying ElarmS with the existing seismic network could provide warning to population centers in repeats of past events. For example, in a repeat of the 1980 Irpinia earthquake Naples could receive an !15- sec warning. The variations in the size of the blind zone and warning times for different regions can be used as a guide to selecting strategic locations for future station deployments.

Description: Published

Description: 495-503

Description: 1.1. TTC - Monitoraggio sismico del territorio nazionale

Description: JCR Journal

Description: reserved

Description: We evaluate the capability of ElarmS to work as an Early Warning System for the next destructive earthquake in Italy. Potential sources were retrieved from the Database of Individual Seismogenic Sources (DISS; http://www.ingv.it/DISS/) and from the catalogue of historical earthquakes (CPTI04; http://emidius.mi.ingv.it/CPTI/). Based on the geometry of the new Italian Broad-Band Seismic Network, we estimate the "Alert time" for each source from different nucleation points and the size of the shadow zone, the area already affected by S-waves. We present our results for peninsular Italy with a particular attention to the metropolitan areas of Napoli, (Southern Italy) that have been selected as a test site for the SAFER project (http://www.saferproject.net/), plus Roma and Firenze.

Description: Not submitted

Description: 4.2. TTC - Scenari e mappe di pericolosità sismica

Description: N/A or not JCR

Description: open

Description: Karlodinium veneficum (syn. Karlodinium micrum, Bergholtz et al. 2006; J Phycol 42:170–193) is a small athecate dinoflagellate commonly present in low levels in temperate, coastal waters. Occasionally, K. veneficum forms ichthyotoxic blooms due to the presence of cytotoxic, hemolytic compounds, putatively named karlotoxins. To evaluate the anti-grazing properties of these karlotoxins, we conducted food removal experiments using the cosmopolitan copepod grazer Acartia tonsa. Wild-caught, adult female A. tonsa were exposed to 6 monoalgal or mixed algal diets made using bloom concentrations of toxic (CCMP 2064) and non-toxic (CSIC1) strains of K. veneficum. Ingestion and clearance rates were calculated using the equations of Frost (1972). Exposure to the toxic strain of K. veneficum did not contribute to an increased mortality of the copepods and no significant differences in copepod mortality were found among the experimental diets. However, A. tonsa had significantly greater clearance and ingestion rates when exposed to a monoalgal diet of the non-toxic strain CSIC1 than when exposed to the monoalgal diet of toxic strain CCMP 2064 and mixed diets dominated by this toxic strain. These results support the hypothesis that karlotoxins in certain strains of K. veneficum deter grazing by potential predators and contribute to the formation and continuation of blooms.

Description: The following report is presented as a statement of progress made at the Massachusetts Institute of Technology (M.I.T.) in the investigation into the possibility of extending the range of reliable weather forecasts. This project has been supported at M.I.T. and other private institutions by Bankhead-Jones appropriations since September, 1937. This report is concerned only with the work completed or in progress at M.I.T. The complementary program now in progress at the Weather Bureau in Washington is referred to only in so far as it has contributed directly to these investigations. Furthermore, the following report refers only to the last two years of the M.I.T. project. The first year of the three-year project was given over principally to the study of the results obtained by long range forecast methods already in use, and to the establishment of a northern hemisphere synoptic weather map procedure as a necessary precedent to the preparation of weekly forecasts on a synoptic basis. The results of the M.I.T. study of certain long range forecast methods already in practice are included in a general survey of such methods already published. The synoptic charts prepared at M.I.T. during that first year of the investigation are listed in an appendix to this report, together with those of the last two years. The preparation of weekly forecasts carried on during a part of that first year was so experimental in nature, and the procedure was so much changed the following year, that the results obtained were considered neither suffciently significant nor comparable enough with the later forecast results to merit any discussion. The present report is divided into three principal sections. Section I presents in condensed form our present conception of the essential nature of the general circulation, and discusses briefly the background of one or two of Professor Rossby's theoretical considerations concerning the general circulation which have found statistical and synoptic application in this investigation. Section II contains in brief form the results of synoptic and statistical checks of a large number of hypothetical relationships which might be assumed to hold in the earth's atmosphere. These include possible relationships in the large scale features of the general circulation, relationships between the general circulation and its different branches or centers of action, between the different branches or centers of action of the general circulation, between characteristics of the general circulation or its branches and anomalies of the meteorological elements in certain regions, between anomalies of the meteorological elements in one region and those in another region, and even between solar activity (sunspots) and characteristics of the general circulation or anomalies of the meteorological elements. The aim was to investigate possible interrelationships of all kinds, either with or without lag, in order to detect as many interaction principles or points as possible in the earth's atmosphere, whether they had direct or only the most indirect bearing on the forecast problem. The relationships investigated applied to daily, weekly, monthly, seasonal, or annual mean conditions. They were selected for investigation either from theoretical or practical considerations of the nature of the general circulation as outlined in Section I, or on the basis of popular beliefs which have long been current among meteorologists, or on the basis of direct observation of data which looked promising. The majority of these hypothetical relationships are found to be quite weak when subjected to rigid statistical checks, but all such results, whether positive or negative, are summarized in this report. Section III outlines the five-day forecast routine practice which has been carried on at M.I.T. during the greater part of the past two years on a weekly basis. It includes a statistical analysis of the verification results. In the conclusion are summarized the results of the investigation which thus far appear significant enough to justify their consideration in five-day or longer range forecasts. Suggestions are offered as to further steps which might profitably be taken if the investigation is to be continued. Finally there is an appendix in which are listed all the daily synoptic maps and mean charts and diagrams of surface and upper air data which have been plotted and analyzed at M.I.T. in connection with this project during the past three years. The importance of such a list is apparent when it is realized that inevitably in an investigation of this kind much the greater part of the time and effort expended is consumed in the routine or semiroutine duties involved in the preparation of such charts.

Description: The present report is intended to cover fully the activities of the long-range forecast project both at the Massachusetts Institute of Technology and at the U. S. Weather Bureau in Washington, between July 1, 1940, and August 1, 1941. It includes all material bearing on the activities of the current fiscal year which has appeared in the three progress reports that were written during the year. The report is in four sections. Section I outlines the administrative set-up of the project and its transfer from the Massachusetts Institute of Technology to the U. S. Weather Bureau in Washington, indicates the general purpose of the project, outlines the program of routine synoptic and statistical work which is maintained as a necessary part of the five-day forecast service, and lists the personnel which has been available to carry on the project. Section II covers in some detail the five-day forecast procedure as practiced during the past year, including one illustrative case selected and discussed by Mr. Namias. The discussion of the five-day forecast procedure is concluded with some remarks on the significance of the results obtained by the basic method and a summary by Mr. Allen of the success of the forecasts as shown by the statistical verification of the forecast temperature and precipitation anomalies. Section III contains a brief discussion of each of the special investigations made during the past year which bear on the five-day forecast problem. For the most part, the results of these investigations were not obtained soon enough to be incorporated in the forecast procedure outlined in Section II. Section IV sets forth recommendations for further theoretical, synoptic and statistical research which is needed to develop and extend the five-day forecasting technique which has been developed by this project.

Description: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hand, K., Phillips, C., Murray, A., Garvin, J., Maize, E., Gibbs, R., Reeves, G., San Martin, A., Tan-Wang, G., Krajewski, J., Hurst, K., Crum, R., Kennedy, B., McElrath, T., Gallon, J., Sabahi, D., Thurman, S., Goldstein, B., Estabrook, P., Lee, S. W., Dooley, J. A., Brinckerhoff, W. B., Edgett, K. S., German, C. R., Hoehler, T. M., Hörst, S. M., Lunine, J. I., Paranicas, C., Nealson, K., Smith, D. E., Templeton, A. S., Russell, M. J., Schmidt, B., Christner, B., Ehlmann, B., Hayes, A., Rhoden, A., Willis, P., Yingst, R. A., Craft, K., Cameron, M. E., Nordheim, T., Pitesky, J., Scully, J., Hofgartner, J., Sell, S. W., Barltrop, K. J., Izraelevitz, J., Brandon, E. J., Seong, J., Jones, J.-P., Pasalic, J., Billings, K. J., Ruiz, J. P., Bugga, R. V., Graham, D., Arenas, L. A., Takeyama, D., Drummond, M., Aghazarian, H., Andersen, A. J., Andersen, K. B., Anderson, E. W., Babuscia, A., Backes, P. G., Bailey, E. S., Balentine, D., Ballard, C. G., Berisford, D. F., Bhandari, P., Blackwood, K., Bolotin, G. S., Bovre, E. A., Bowkett, J., Boykins, K. T., Bramble, M. S., Brice, T. M., Briggs, P., Brinkman, A. P., Brooks, S. M., Buffington, B. B., Burns, B., Cable, M. L., Campagnola, S., Cangahuala, L. A., Carr, G. A., Casani, J. R., Chahat, N. E., Chamberlain-Simon, B. K., Cheng, Y., Chien, S. A., Cook, B. T., Cooper, M., DiNicola, M., Clement, B., Dean, Z., Cullimore, E. A., Curtis, A. G., Croix, J-P. de la, Pasquale, P. Di, Dodd, E. M., Dubord, L. A., Edlund, J. A., Ellyin, R., Emanuel, B., Foster, J. T., Ganino, A. J., Garner, G. J., Gibson, M. T., Gildner, M., Glazebrook, K. J., Greco, M. E., Green, W. M., Hatch, S. J., Hetzel, M. M., Hoey, W. A., Hofmann, A. E., Ionasescu, R., Jain, A., Jasper, J. D., Johannesen, J. R., Johnson, G. K., Jun, I., Katake, A. B., Kim-Castet, S. Y., Kim, D. I., Kim, W., Klonicki, E. F., Kobeissi, B., Kobie, B. D., Kochocki, J., Kokorowski, M., Kosberg, J. A., Kriechbaum, K., Kulkarni, T. P., Lam, R. L., Landau, D. F., Lattimore, M. A., Laubach, S. L., Lawler, C. R., Lim, G., Lin, J. Y., Litwin, T. E., Lo, M. W., Logan, C. A., Maghasoudi, E., Mandrake, L., Marchetti, Y., Marteau, E., Maxwell, K. A., Namee, J. B. Mc, Mcintyre, O., Meacham, M., Melko, J. P., Mueller, J., Muliere, D. A., Mysore, A., Nash, J., Ono, H., Parker, J. M., Perkins, R. C., Petropoulos, A. E., Gaut, A., Gomez, M. Y. Piette, Casillas, R. P., Preudhomme, M., Pyrzak, G., Rapinchuk, J., Ratliff, J. M., Ray, T. L., Roberts, E. T., Roffo, K., Roth, D. C., Russino, J. A., Schmidt, T. M., Schoppers, M. J., Senent, J. S., Serricchio, F., Sheldon, D. J., Shiraishi, L. R., Shirvanian, J., Siegel, K. J., Singh, G., Sirota, A. R., Skulsky, E. D., Stehly, J. S., Strange, N. J., Stevens, S. U., Sunada, E. T., Tepsuporn, S. P., Tosi, L. P. C., Trawny, N., Uchenik, I., Verma, V., Volpe, R. A., Wagner, C. T., Wang, D., Willson, R. G., Wolff, J. L., Wong, A. T., Zimmer, A. K., Sukhatme, K. G., Bago, K. A., Chen, Y., Deardorff, A. M., Kuch, R. S., Lim, C., Syvertson, M. L., Arakaki, G. A., Avila, A., DeBruin, K. J., Frick, A., Harris, J. R., Heverly, M. C., Kawata, J. M., Kim, S.-K., Kipp, D. M., Murphy, J., Smith, M. W., Spaulding, M. D., Thakker, R., Warner, N. Z., Yahnker, C. R., Young, M. E., Magner, T., Adams, D., Bedini, P., Mehr, L., Sheldon, C., Vernon, S., Bailey, V., Briere, M., Butler, M., Davis, A., Ensor, S., Gannon, M., Haapala-Chalk, A., Hartka, T., Holdridge, M., Hong, A., Hunt, J., Iskow, J., Kahler, F., Murray, K., Napolillo, D., Norkus, M., Pfisterer, R., Porter, J., Roth, D., Schwartz, P., Wolfarth, L., Cardiff, E. H., Davis, A., Grob, E. W., Adam, J. R., Betts, E., Norwood, J., Heller, M. M., Voskuilen, T., Sakievich, P., Gray, L., Hansen, D. J., Irick, K. W., Hewson, J. C., Lamb, J., Stacy, S. C., Brotherton, C. M., Tappan, A. S., Benally, D., Thigpen, H., Ortiz, E., Sandoval, D., Ison, A. M., Warren, M., Stromberg, P. G., Thelen, P. M., Blasy, B., Nandy, P., Haddad, A. W., Trujillo, L. B., Wiseley, T. H., Bell, S. A., Teske, N. P., Post, C., Torres-Castro, L., Grosso, C. Wasiolek, M. Science goals and mission architecture of the Europa Lander mission concept. The Planetary Science Journal, 3(1), (2022): 22, https://doi.org/10.3847/psj/ac4493.

Description: Europa is a premier target for advancing both planetary science and astrobiology, as well as for opening a new window into the burgeoning field of comparative oceanography. The potentially habitable subsurface ocean of Europa may harbor life, and the globally young and comparatively thin ice shell of Europa may contain biosignatures that are readily accessible to a surface lander. Europa's icy shell also offers the opportunity to study tectonics and geologic cycles across a range of mechanisms and compositions. Here we detail the goals and mission architecture of the Europa Lander mission concept, as developed from 2015 through 2020. The science was developed by the 2016 Europa Lander Science Definition Team (SDT), and the mission architecture was developed by the preproject engineering team, in close collaboration with the SDT. In 2017 and 2018, the mission concept passed its mission concept review and delta-mission concept review, respectively. Since that time, the preproject has been advancing the technologies, and developing the hardware and software, needed to retire risks associated with technology, science, cost, and schedule.

Description: K.P.H., C.B.P., E.M., and all authors affiliated with the Jet Propulsion Laboratory carried out this research at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration (grant No. 80NM0018D0004). J.I.L. was the David Baltimore Distinguished Visiting Scientist during the preparation of the SDT report. JPL/Caltech2021.