In:
Journal of Hypertension, Ovid Technologies (Wolters Kluwer Health), Vol. 41, No. 10 ( 2023-10), p. 1645-1652
Abstract:
This study aimed to explore whether 99m Tc-radiolabeled fibroblast activation protein inhibitor ( 99m Tc-HFAPi) imaging can detect early myocardial fibrosis in the hypertensive heart. Methods: In the experimental model, spontaneously hypertensive rats (SHRs) and age-matched Wistar Kyoto rats (WKYs) were randomly divided into three groups (8, 16, and 28 weeks). The animals underwent 99m Tc-HFAPi imaging and echocardiography. Autoradiography and histological analyses were performed in the left ventricle. The mRNA and protein expression level of the fibroblast activation protein (FAP) and collagen I were measured using quantitative PCR and western blot. In the clinical investigation, a total of 106 patients with essential hypertension and 20 gender-matched healthy controls underwent 99m Tc-HFAPi imaging and echocardiography. Results: In-vivo and in-vitro autographic images demonstrated diffusely enhanced 99m Tc-HFAPi uptake in the SHR heart starting at week 8, before irreversible collagen deposition. The mRNA and protein levels of FAP in SHRs began to increase from week 8, whereas changes in collagen I levels were not detected until week 28. In the clinical investigation, even in hypertensive patients with normal diastolic indicators, normal left ventricular geometry, and normal global longitudinal strain (GLS), the prevalence of increased 99m Tc-HFAPi uptake reached 34, 41, and 20%, respectively, indicating that early fibrogenesis precedes structural and functional myocardial abnormalities. Conclusion: In hypertension, 99m Tc-HFAPi imaging can detect early fibrotic process before myocardial functional and structural changes.
Type of Medium:
Online Resource
ISSN:
0263-6352
,
1473-5598
DOI:
10.1097/HJH.0000000000003517
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2023
detail.hit.zdb_id:
2017684-3
Permalink