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  • 1
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    Online Resource
    Heterotopic ovarian allotransplantation in a caprine model: Effects of implant site on morphological parameters
    Elsevier BV ; 2024
    In:  Animal Reproduction Science Vol. 267 ( 2024-08), p. 107509-
    Details
    In: Animal Reproduction Science, Elsevier BV, Vol. 267 ( 2024-08), p. 107509-
    Type of Medium: Online Resource
    ISSN: 0378-4320
    URL: Article
    DOI: 10.1016/j.anireprosci.2024.107509
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2024
    detail.hit.zdb_id: 1495854-5
    SSG: 22
    SSG: 12
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  • 2
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    Influência das fases de anestro e diestro na competência meiótica oocitária de cadelas
    Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais ; 2015
    In:  Brazilian Journal of Veterinary Research and Animal Science Vol. 52, No. 3 ( 2015-09-30), p. 266-
    Details
    In: Brazilian Journal of Veterinary Research and Animal Science, Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais, Vol. 52, No. 3 ( 2015-09-30), p. 266-
    Abstract: 〈 p 〉 A cadela apresenta particularidades reprodutivas que a diferencia de outras espécies. Diversos experimentos têm sido realizados visando estabelecer protocolos eficientes para a maturação, entretanto os resultados mostram-se insatisfatórios. Nesse aspecto, a fase reprodutiva da fêmea doadora deve ser considerada, já que pode ser um fator de variabilidade dos achados ate então presenciados nessa espécie. O objetivo deste estudo foi avaliar a influencia das fases do ciclo estral (anestro e diestro) na maturação 〈 em 〉 in vitro 〈 /em 〉 (MIV) de cadelas. Os ovários foram transportados em solução de cloreto de sódio 0,9% e seccionados em solução salina fosfato tamponado (PBS) e os complexos 〈 em 〉 cumulus 〈 /em 〉 -oócito (COCs) selecionados em meio de cultura de tecidos (TCM) 199 suplementado com HEPES. Foram obtidos 469 oócitos grau 1 de cadelas em anestro e diestro. Esses oócitos selecionados foram transferidos para o meio de maturação por um período de 72 horas, sendo posteriormente submetidos a solução de hialuronidase e corados com HOESCHT 33342 para avaliação da configuração nuclear. A comparação das fases de anestro e diestro não revelou diferença (p & gt; 0,05) entre os estágios de maturação nuclear. Dessa maneira, a fase do ciclo estral não influenciou na maturação in vitro de oócitos caninos, incrementando os índices de M-II nesta espécie. 〈 /p 〉
    Type of Medium: Online Resource
    ISSN: 1678-4456 , 1413-9596
    URL: Article
    DOI: 10.11606/issn.1678-4456.v52i3p266-272
    Language: Unknown
    Publisher: Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais
    Publication Date: 2015
    detail.hit.zdb_id: 2053101-1
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  • 3
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    Online Resource
    Effect of oxygen tension on in vitro viability and development of dog follicles enclosed within the ovarian cortex
    Wiley ; 2019
    In:  Reproduction in Domestic Animals Vol. 54, No. 8 ( 2019-08), p. 1139-1144
    Details
    In: Reproduction in Domestic Animals, Wiley, Vol. 54, No. 8 ( 2019-08), p. 1139-1144
    Abstract: Oxygen concentration has been shown to influence in vitro viability and growth of ovarian follicles. The present study examined the effect of oxygen tension on in vitro development of dog follicles enclosed within the ovarian cortex. Ovaries were obtained from domestic dogs (age, 8 months to 2 years), and cortical fragments were recovered. The cortices were then incubated on 1.5% (w/v) agarose gel blocks within a 4‐well culture plate containing Eagle Minimum Essential Medium (MEM). Ovarian follicles within the tissues were processed for histology and assessed for follicle density, viability and diameter immediately after collection (Control) or after 2 or 5 days of in vitro incubation. Apoptotic cells were assessed using TUNEL assay. Comparisons of follicular viability and diameter were performed using analysis of variance followed by Tukey's test ( p   〈  0.05). Comparisons of follicle density and apoptosis among treatments were conducted using Non‐parametric Kruskal–Wallis test followed by Friedman's test ( p   〈  0.05). No difference ( p   〉  0.05) in follicle density was observed among groups at Day 2 of in vitro culture. However, the density of follicles within cortices cultured in 20% oxygen for 5 days significantly reduced compared to the Control and those incubated in 5% concentration. The viability of cultured follicles in all treatments decreased ( p   〈  0.05) compared to the Control after 2 days incubation, and this value further reduced ( p   〈  0.05) in 20% oxygen group at Day 5. There were no differences in the percentages of apoptotic follicles between the two treatment groups ( p   〉  0.05). Nevertheless, after 5 days of culture, the percentage of TUNEL‐positive follicles increased significantly ( p   〈  0.05) in cortices incubated in 20% oxygen environment. In conclusion, our findings demonstrated that 5% oxygen level was superior to 20% concentration in sustaining in vitro viability of dog follicles enclosed within the ovarian cortex.
    Type of Medium: Online Resource
    ISSN: 0936-6768 , 1439-0531
    URL: Issue
    URL: Article
    DOI: 10.1111/rda.v54.8
    DOI: 10.1111/rda.13482
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2020494-2
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  • 4
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    Online Resource
    INFLUÊNCIA DO HEPES NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS OBTIDOS DE CADELAS EM DIESTRO E ANESTRO
    FapUNIFESP (SciELO) ; 2019
    In:  Ciência Animal Brasileira Vol. 20 ( 2019)
    Details
    In: Ciência Animal Brasileira, FapUNIFESP (SciELO), Vol. 20 ( 2019)
    Abstract: Abstract Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The aim of this study was to evaluate the effect of HEPES on the in vitro maturation of dog oocytes in anestrus and diestrus. Ovaries were collected, aseptically isolated and transported refrigerated at a temperature of 4ºC. Cumulus-oocyte complexe (COCs) from the two phases of estrous cycle were subjected to two treatments: medium TCM-199 with addition of 25 mM HEPES (TG) and medium without supplementation (CG). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the anestrus and diestrus phases of the TG showed a higher frequency of oocytes in metaphase II (M-II) (p 〈 0.01) stage.Comparing the different reproductive status, it was observed that the oocytes obtained from the diestrus phase presented higher rates of GVBD (germinal vesicle breakdown) and M-II. Our results showed that HEPES preserves the viability and oocyte morphology essential for the acquisition of meiotic competence, increasing the M-II rates and that the oocytes obtained from the diestrus phase are better able to complete oocyte maturation.
    Type of Medium: Online Resource
    ISSN: 1809-6891 , 1518-2797
    URL: Article
    DOI: 10.1590/1809-6891v20e-53372
    Language: Portuguese
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2019
    detail.hit.zdb_id: 2404787-9
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  • 5
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    First proteomic analysis of diestrus and anestrus canine oocytes at the germinal vesicle reveals candidate proteins involved in oocyte meiotic competence
    Wiley ; 2019
    In:  Reproduction in Domestic Animals Vol. 54, No. 12 ( 2019-12), p. 1532-1542
    Details
    In: Reproduction in Domestic Animals, Wiley, Vol. 54, No. 12 ( 2019-12), p. 1532-1542
    Abstract: In domestic dogs, oocyte maturation rates are low and the percentage of oocytes that remain in the stage of germinal vesicle (GV) regardless of culture conditions is high. The present study was conducted to characterize the proteome of canine oocyte at the germinal vesicle stage using label‐free mass spectrometry. Ovaries were collected from 415 adult domestic dogs and oocytes were divided anestrus and diestrus group. Protein lysates were subjected to quantitative proteomic analysis to identify differentially expressed proteins in different status reproductive. All runs for each sample were performed on an Easy nLC1000 nano‐LC chromatograph system directly connected to a quadrupole‐type Orbitrap mass spectrometer. For identification of peptides and proteins, raw data of the spectra were loaded into MaxQuant software version 1.5.2.8. Proteomic data were analysed according to gene ontology and a protein–protein interaction network. 312 proteins were identified and grouped according to their biological processes, molecular functions and cellular component. Forty‐six differentially expressed proteins among diestrus and control group were associated with at least one GO term in the biological process database. Several proteins involved in the cell cycle, fertilization, regulation of transcription and signalling pathways that are essential for the full development of oocytes and fertilization were expressed. This study identified proteins that were absent, and more or less expressed in different status reproductive. These differentially expressed proteins revealed a framework of molecular reorganization within a GV that renders its competency. This knowledge will enable the identification of target competence biomarkers and thus the establishment of more adequate means of cultivation to improve the M‐I and II indexes in this species and also to better understand the physiology of the domestic dog, promoting the development of new reproduction biotechniques.
    Type of Medium: Online Resource
    ISSN: 0936-6768 , 1439-0531
    URL: Issue
    URL: Article
    DOI: 10.1111/rda.v54.12
    DOI: 10.1111/rda.13560
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2020494-2
    SSG: 12
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  • 6
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    Efeito do fator de crescimento epidermal (EGF) na maturação in vitro de oócitos caninos
    Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais ; 2014
    In:  Brazilian Journal of Veterinary Research and Animal Science Vol. 51, No. 2 ( 2014-09-23), p. 158-
    Details
    In: Brazilian Journal of Veterinary Research and Animal Science, Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais, Vol. 51, No. 2 ( 2014-09-23), p. 158-
    Type of Medium: Online Resource
    ISSN: 1678-4456 , 1413-9596
    URL: Article
    DOI: 10.11606/issn.2318-3659.v51i2p158-165
    Language: Unknown
    Publisher: Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais
    Publication Date: 2014
    detail.hit.zdb_id: 2053101-1
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  • 7
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    Influência das fases de anestro e diestro sobre a configuração da cromatina em vesícula germinativa de oócitos caninos
    Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais ; 2013
    In:  Brazilian Journal of Veterinary Research and Animal Science Vol. 50, No. 6 ( 2013-12-09), p. 474-
    Details
    In: Brazilian Journal of Veterinary Research and Animal Science, Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais, Vol. 50, No. 6 ( 2013-12-09), p. 474-
    Type of Medium: Online Resource
    ISSN: 1678-4456 , 1413-9596
    URL: Article
    DOI: 10.11606/issn.1678-4456.v50i6p474-481
    Language: Unknown
    Publisher: Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais
    Publication Date: 2013
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  • 8
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    Online Resource
    Influência da temperatura de transporte de ovários na maturação in vitro de oócitos caninos coletados em diferentes estágios do ciclo estral
    Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais ; 2015
    In:  Brazilian Journal of Veterinary Research and Animal Science Vol. 52, No. 2 ( 2015-06-30), p. 158-
    Details
    In: Brazilian Journal of Veterinary Research and Animal Science, Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais, Vol. 52, No. 2 ( 2015-06-30), p. 158-
    Abstract: 〈 p 〉 Foi avaliada a influencia do ciclo estral e temperatura de transporte de ovários na maturação 〈 em 〉 in vitro 〈 /em 〉 de oócitos caninos. As cadelas foram categorizadas em dois grupos baseados no estagio do ciclo estral – anestro ou diestro. Um ovário por par coletado foi transportado em solução fisiológica 0,9% a 4°C enquanto o outro foi transportado a 37°C. Então, os ovários foram seccionados em PBS para a liberação dos complexos 〈 em 〉 cumulus 〈 /em 〉 oocito (COCs). Um total de 345 COCs (n = 186 oocitos obtidos de cadelas em anestro e 159 em diestro) foi cultivado em TCM 199 suplementado com HEPES, piruvato de sódio, cisteina, hormônio folículo estimulante (FSH), gonadotrofina coriônica humana (hCG), estrógeno (E2) e fator de crescimento epidermal (EGF). Apos 72h de maturação, os COCs foram desnudados, fixados e corados para avaliação da maturação nuclear. O teste de Fisher foi utilizado para avaliar as diferenças entre os grupos. O nível de significância adotado foi de 0,05. Os oócitos obtidos de cadelas em diestro transportados a 4°C apresentaram maior frequência de oócitos no estagio de metáfase II (21,1%) que os mantidos na temperatura de 37°C (p & lt; 0,01). De forma similar, houve maior frequência de oócitos nos estágios de metáfase II (11,2%) nos ovários obtidos de cadelas em anestro e transportados a 4°C que nos ovários mantidos a 37°C (p & lt; 0,05). Concluiu-se que a temperatura de transporte influencia os resultados de viabilidade oocitária canina e a maturação 〈 em 〉 in vitro 〈 /em 〉 , independentemente do estagio reprodutivo da fêmea. 〈 /p 〉
    Type of Medium: Online Resource
    ISSN: 1678-4456 , 1413-9596
    URL: Article
    DOI: 10.11606/issn.1678-4456.v52i2p158-166
    Language: Unknown
    Publisher: Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais
    Publication Date: 2015
    detail.hit.zdb_id: 2053101-1
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  • 9
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    Online Resource
    Escolha dos melhores parâmetros para predizer a data do parto em cadelas de diferentes portes por meio da avaliação ultrassonográfica fetal.
    GN1 Sistemas e Publicacoes Ltd. ; 2022
    In:  Revista Brasileira de Higiene e Sanidade Animal Vol. 16, No. 2 ( 2022)
    Details
    In: Revista Brasileira de Higiene e Sanidade Animal, GN1 Sistemas e Publicacoes Ltd., Vol. 16, No. 2 ( 2022)
    Type of Medium: Online Resource
    ISSN: 1981-2965
    URL: Article
    DOI: 10.5935/1981-2965.20220011
    Language: Unknown
    Publisher: GN1 Sistemas e Publicacoes Ltd.
    Publication Date: 2022
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  • 10
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    Online Resource
    Influence of epidermal growth factor (EGF) supplementation at different times of 〈i〉in vitro〈/i〉 maturation of canine oocytes
    Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais ; 2017
    In:  Brazilian Journal of Veterinary Research and Animal Science Vol. 54, No. 4 ( 2017-12-31), p. 388-397
    Details
    In: Brazilian Journal of Veterinary Research and Animal Science, Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais, Vol. 54, No. 4 ( 2017-12-31), p. 388-397
    Abstract: The aim of this study was to evaluate the influence of epidermal growth factor (EGF) on in vitro maturation of canine oocytes at different times of the process. Ovaries were collected from 55 bitches considered healthy and aseptically isolated, immersed in physiological solution (0.9% NaCl) and transported under refrigeration. Grade 1 cumulus-oocyte complexes (COCs) were selected and divided into two groups: control group (CG) and treatment group (TG). In CG 698 grade I COCs were placed in 4-well plates containing TCM-199 medium supplemented with 25 mM HEPES, 100 IU/mL penicillin, 100 mg/mL streptomycin, 26 mM sodium bicarbonate, 1.5 mM sodium pyruvate, 2.9 mM sodium lactate pentahydrate, 0.6 mM cysteine, 0.03 IU/mL hCG, 0.5 μg/mL FSH, 20 μg/mL estrogen at 38.5ºC in a humidified atmosphere of 5% CO2 in times of 24 h, 48 h, and 72 h. In TG 547 COCs received the same maturation medium plus 10 ηg/mL EGF. Logistic regression models (SAS, 2011) were constructed in order to estimate the chances of oocytes being observed at nuclear maturation stages in different culture times (24 h, 48 h, and 72 h). Based on the results found EGF-supplemented medium showed 2.56 times more chances of having an oocyte at metaphase I (M-I) than medium without EGF (p 〈 0.0001). The results of this study demonstrated that the time of 72 h showed 5.88 times more chances of having an oocyte at metaphase II (M-II) compared to time of 24 h (p = 0.0001) and 7.69 times more chance than time of 48 h (p = 0.0001). The chances of finding an oocyte at M-II were also 9.09 times higher in medium supplemented with EGF than in medium without EGF (p = 0.0001). Thus, these results demonstrated the essential importance of EGF at different moments of oocyte maturation, being a key component for the acquisition of meiotic competence in bitches, increasing the M-I and M-II rates.
    Type of Medium: Online Resource
    ISSN: 1678-4456 , 1413-9596
    URL: Article
    DOI: 10.11606/issn.1678-4456.bjvras.2017.133103
    Language: Unknown
    Publisher: Universidade de São Paulo. Agência de Bibliotecas e Coleções Digitais
    Publication Date: 2017
    detail.hit.zdb_id: 2053101-1
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