In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 4 ( 2021-4-19), p. e3001144-
Abstract:
Delineating human cardiac pathologies and their basic molecular mechanisms relies on research conducted in model organisms. Yet translating findings from preclinical models to humans present a significant challenge, in part due to differences in cardiac protein expression between humans and model organisms. Proteins immediately determine cellular function, yet their large-scale investigation in hearts has lagged behind those of genes and transcripts. Here, we set out to bridge this knowledge gap: By analyzing protein profiles in humans and commonly used model organisms across cardiac chambers, we determine their commonalities and regional differences. We analyzed cardiac tissue from each chamber of human, pig, horse, rat, mouse, and zebrafish in biological replicates. Using mass spectrometry–based proteomics workflows, we measured and evaluated the abundance of approximately 7,000 proteins in each species. The resulting knowledgebase of cardiac protein signatures is accessible through an online database: atlas.cardiacproteomics.com . Our combined analysis allows for quantitative evaluation of protein abundances across cardiac chambers, as well as comparisons of cardiac protein profiles across model organisms. Up to a quarter of proteins with differential abundances between atria and ventricles showed opposite chamber-specific enrichment between species; these included numerous proteins implicated in cardiac disease. The generated proteomics resource facilitates translational prospects of cardiac studies from model organisms to humans by comparisons of disease-linked protein networks across species.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001144
DOI:
10.1371/journal.pbio.3001144.g001
DOI:
10.1371/journal.pbio.3001144.g002
DOI:
10.1371/journal.pbio.3001144.g003
DOI:
10.1371/journal.pbio.3001144.g004
DOI:
10.1371/journal.pbio.3001144.g005
DOI:
10.1371/journal.pbio.3001144.s001
DOI:
10.1371/journal.pbio.3001144.s002
DOI:
10.1371/journal.pbio.3001144.s003
DOI:
10.1371/journal.pbio.3001144.s004
DOI:
10.1371/journal.pbio.3001144.s005
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10.1371/journal.pbio.3001144.s006
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10.1371/journal.pbio.3001144.s007
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10.1371/journal.pbio.3001144.s008
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10.1371/journal.pbio.3001144.s009
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10.1371/journal.pbio.3001144.s010
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10.1371/journal.pbio.3001144.s011
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10.1371/journal.pbio.3001144.s012
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10.1371/journal.pbio.3001144.s013
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10.1371/journal.pbio.3001144.s014
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10.1371/journal.pbio.3001144.s015
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10.1371/journal.pbio.3001144.s016
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10.1371/journal.pbio.3001144.s017
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10.1371/journal.pbio.3001144.s018
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10.1371/journal.pbio.3001144.s019
DOI:
10.1371/journal.pbio.3001144.s020
DOI:
10.1371/journal.pbio.3001144.s021
DOI:
10.1371/journal.pbio.3001144.s022
DOI:
10.1371/journal.pbio.3001144.s023
DOI:
10.1371/journal.pbio.3001144.s024
DOI:
10.1371/journal.pbio.3001144.s025
DOI:
10.1371/journal.pbio.3001144.s026
DOI:
10.1371/journal.pbio.3001144.s027
DOI:
10.1371/journal.pbio.3001144.s028
DOI:
10.1371/journal.pbio.3001144.r001
DOI:
10.1371/journal.pbio.3001144.r002
DOI:
10.1371/journal.pbio.3001144.r003
DOI:
10.1371/journal.pbio.3001144.r004
DOI:
10.1371/journal.pbio.3001144.r005
DOI:
10.1371/journal.pbio.3001144.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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