In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 14, No. 12 ( 2020-12-31), p. e0008867-
Abstract:
Aedes aegypti mosquito-borne viruses including Zika (ZIKV), dengue (DENV), yellow fever (YFV), and chikungunya (CHIKV) have emerged and re-emerged globally, resulting in an elevated burden of human disease. Aedes aegypti is found worldwide in tropical, sub-tropical, and temperate areas. The characterization of mosquito blood meals is essential to understand the transmission dynamics of mosquito-vectored pathogens. Methodology/principal findings Here, we report Ae . aegypti and Culex quinquefasciatus host feeding patterns and arbovirus transmission in Northern Mexico using a metabarcoding-like approach with next-generation deep sequencing technology. A total of 145 Ae . aegypti yielded a blood meal analysis result with 107 (73.8%) for a single vertebrate species and 38 (26.2%) for two or more. Among the single host blood meals for Ae . aegypti , 28.0% were from humans, 54.2% from dogs, 16.8% from cats, and 1.0% from tortoises. Among those with more than one species present, 65.9% were from humans and dogs. For Cx . quinquefasciatus , 388 individuals yielded information with 326 (84%) being from a single host and 63 (16.2%) being from two or more hosts. Of the single species blood meals, 77.9% were from dogs, 6.1% from chickens, 3.1% from house sparrows, 2.4% from humans, while the remaining 10.5% derived from other 12 host species. Among those which had fed on more than one species, 11% were from dogs and humans, and 89% of other host species combinations. Forage ratio analysis revealed dog as the most over-utilized host by Ae . aegypti (= 4.3) and Cx . quinquefasciatus (= 5.6) and the human blood index at 39% and 4%, respectively. A total of 2,941 host-seeking female Ae . aegypti and 3,536 Cx . quinquefasciatus mosquitoes were collected in the surveyed area. Of these, 118 Ae . aegypti pools and 37 Cx . quinquefasciatus pools were screened for seven arboviruses (ZIKV, DENV 1–4, CHIKV, and West Nile virus (WNV)) using qRT-PCR and none were positive (point prevalence = 0%). The 95%-exact upper limit confidence interval was 0.07% and 0.17% for Ae . aegypti and Cx . quinquefasciatus , respectively Conclusions/significance The low human blood feeding rate in Ae . aegypti , high rate of feeding on mammals by Cx . quinquefasciatus , and the potential risk to transmission dynamics of arboviruses in highly urbanized areas of Northern Mexico is discussed.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0008867
DOI:
10.1371/journal.pntd.0008867.g001
DOI:
10.1371/journal.pntd.0008867.g002
DOI:
10.1371/journal.pntd.0008867.g003
DOI:
10.1371/journal.pntd.0008867.g004
DOI:
10.1371/journal.pntd.0008867.t001
DOI:
10.1371/journal.pntd.0008867.t002
DOI:
10.1371/journal.pntd.0008867.t003
DOI:
10.1371/journal.pntd.0008867.t004
DOI:
10.1371/journal.pntd.0008867.s001
DOI:
10.1371/journal.pntd.0008867.s002
DOI:
10.1371/journal.pntd.0008867.s003
DOI:
10.1371/journal.pntd.0008867.s004
DOI:
10.1371/journal.pntd.0008867.s005
DOI:
10.1371/journal.pntd.0008867.s006
DOI:
10.1371/journal.pntd.0008867.r001
DOI:
10.1371/journal.pntd.0008867.r002
DOI:
10.1371/journal.pntd.0008867.r003
DOI:
10.1371/journal.pntd.0008867.r004
DOI:
10.1371/journal.pntd.0008867.r005
DOI:
10.1371/journal.pntd.0008867.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2429704-5
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