In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 2 ( 2021-2-26), p. e1009352-
Abstract:
Serological and plasmablast responses and plasmablast-derived IgG monoclonal antibodies (MAbs) have been analysed in three COVID-19 patients with different clinical severities. Potent humoral responses were detected within 3 weeks of onset of illness in all patients and the serological titre was elicited soon after or concomitantly with peripheral plasmablast response. An average of 13.7% and 3.5% of plasmablast-derived MAbs were reactive with virus spike glycoprotein or nucleocapsid, respectively. A subset of anti-spike (10 of 32) antibodies cross-reacted with other betacoronaviruses tested and harboured extensive somatic mutations, indicative of an expansion of memory B cells upon SARS-CoV-2 infection. Fourteen of 32 anti-spike MAbs, including five anti-receptor-binding domain (RBD), three anti-non-RBD S1 and six anti-S2, neutralised wild-type SARS-CoV-2 in independent assays. Anti-RBD MAbs were further grouped into four cross-inhibiting clusters, of which six antibodies from three separate clusters blocked the binding of RBD to ACE2 and five were neutralising. All ACE2-blocking anti-RBD antibodies were isolated from two recovered patients with prolonged fever, which is compatible with substantial ACE2-blocking response in their sera. Finally, the identification of non-competing pairs of neutralising antibodies would offer potential templates for the development of prophylactic and therapeutic agents against SARS-CoV-2.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009352
DOI:
10.1371/journal.ppat.1009352.g001
DOI:
10.1371/journal.ppat.1009352.g002
DOI:
10.1371/journal.ppat.1009352.g003
DOI:
10.1371/journal.ppat.1009352.t001
DOI:
10.1371/journal.ppat.1009352.t002
DOI:
10.1371/journal.ppat.1009352.t003
DOI:
10.1371/journal.ppat.1009352.s001
DOI:
10.1371/journal.ppat.1009352.s002
DOI:
10.1371/journal.ppat.1009352.s003
DOI:
10.1371/journal.ppat.1009352.s004
DOI:
10.1371/journal.ppat.1009352.s005
DOI:
10.1371/journal.ppat.1009352.s006
DOI:
10.1371/journal.ppat.1009352.s007
DOI:
10.1371/journal.ppat.1009352.s008
DOI:
10.1371/journal.ppat.1009352.s009
DOI:
10.1371/journal.ppat.1009352.s010
DOI:
10.1371/journal.ppat.1009352.s011
DOI:
10.1371/journal.ppat.1009352.s012
DOI:
10.1371/journal.ppat.1009352.s013
DOI:
10.1371/journal.ppat.1009352.s014
DOI:
10.1371/journal.ppat.1009352.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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