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  • 11
    Electronic Resource
    Electronic Resource
    Riboprinting: A Tool for the Study of Genetic Diversity in Microorganisms (1997)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 44 (1997), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Classical morphology-based methods of taxonomic and phylogenetic analysis are inadequate in many groups of structurally simple eukaryotes. Molecular methods can generate data independently of the complexity of the organisms’ morphology. Riboprinting is one such technique, and involves restriction enzyme analysis of polymerase chain reaction amplified small subunit ribosomal RNA genes. The utility of the method is illustrated with examples from several genera of intestinal and bloodstream parasites. Among the applications of riboprinting are the detection of cryptic genetic variation within species, organism misidentifications and culture mix-ups, independent verification of DNA sequences, and the rapid generation of data useful in phylogenetic analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1997.tb05667.x
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  • 12
    Electronic Resource
    Electronic Resource
    Analysis of Genes of Mitochondrial Origin in the Genus Entamoeba (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . The amitochondriate protistan parasite Entamoeba histolytica has lost most mitochondria functions secondarily but has retained a reduced organelle of mitochondrial origin, the mitosome. We here investigate the presence, origins, and expression in other species of Entamoeba of three genes of mitochondrial origin—pyridine nucleotide transhydrogenase and the mitochondrial-type chaperonins cpn60 and hsp70. The genes appear to be present in all species and specifically related, confirming that the E. histolytica mitosomal genes were not acquired recently by lateral transfer from another organism. Detection of expression was not possible in all cases under the culture conditions used, but several genes were induced during recovery from exposure to a heat shock. This includes the transhydrogenase, which to our knowledge has not been shown previously to be a heat-shock protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00119.x
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  • 13
    Electronic Resource
    Electronic Resource
    Ribosomal RNA evolution by fragmentation of the 23S progenitor: Maturation pathway parallels evolutionary emergence (1982)
    Springer
    Journal of molecular evolution 18 (1982), S. 329-336 
    Details
    ISSN: 1432-1432
    Keywords: Ribosomal 5.8S and 4.5S RNAs ; rRNA phylogeny and precursor maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The eukaryotic 5.8S and the chloroplast 4.5S ribosomal RNAs were proposed to have arisen from the 5′ and 3′ ends respectively of prokaryotic 23S ribosomal RNA by the introduction of new processing sites during evolution. This hypothesis was supported by comparison of previously published primary sequences; in addition we can draw models of secondary structure in accord with this notion. Finally, we further noted that the sequence of processing cuts in the maturation pathway of ribosomal RNA reflects the probable order in which they arose during evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01733899
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  • 14
    Electronic Resource
    Electronic Resource
    Human parasite finds taxonomic home (1996)
    [s.l.] : Nature Publishing Group
    Nature 380 (1996), S. 398-398 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR - The phylogenetic analysis of ribo-somal RNAs from Blastocystis hominis provides another interesting example of how molecular data can elucidate the taxonomic affinity of an organism that has proved intractable by traditional techniques. B. hominis, an obligately anaerobic protist inhabiting ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/380398a0
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