GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Author Correction: Robust estimation of bacterial cell count from optical density
    Springer Science and Business Media LLC ; 2020
    In:  Communications Biology Vol. 3, No. 1 ( 2020-10-27)
    Details
    In: Communications Biology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2020-10-27)
    Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.
    Type of Medium: Online Resource
    ISSN: 2399-3642
    URL: Article
    DOI: 10.1038/s42003-020-01371-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2919698-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Robust estimation of bacterial cell count from optical density
    Springer Science and Business Media LLC ; 2020
    In:  Communications Biology Vol. 3, No. 1 ( 2020-09-17)
    Details
    In: Communications Biology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2020-09-17)
    Abstract: Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli . Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals   〈 1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
    Type of Medium: Online Resource
    ISSN: 2399-3642
    URL: Article
    DOI: 10.1038/s42003-020-01127-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2919698-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Efficacy of ultrasound therapy for the treatment of lateral elbow tendinopathy (the UCICLET Trial): study protocol for a three-arm, prospective, multicentre, randomised controlled trial
    BMJ ; 2022
    In:  BMJ Open Vol. 12, No. 1 ( 2022-01), p. e057266-
    Details
    In: BMJ Open, BMJ, Vol. 12, No. 1 ( 2022-01), p. e057266-
    Abstract: Lateral elbow tendinopathy (LET) is a highly prevalent disease among the middle-aged population, with no consensus on optimal management. Non-operative treatment is generally accepted as the first-line intervention. Ultrasound (US) therapy has been reported to be beneficial for various orthopaedic diseases, including tendinopathy. The purpose of this study is to investigate the efficacy of US for LET treatment. Methods and analysis This protocol entails a three-arm, prospective, multicentre, randomised controlled trial. Seventy-two eligible participants with clinically confirmed LET will be assigned to either (1) US, (2) corticosteroid injections or (3) control group. All participants will receive exercise-based therapy as a fundamental intervention. The primary outcome is Patient-rated Tennis Elbow Evaluation. The secondary outcomes include Visual Analogue Scale for pain, shortened version of the Disabilities of the Arm, Shoulder and Hand for upper limb disability, pain free/maximum grip strength, Work Limitations Questionnaire-25 for functional limitations at work, EuroQol-5D for general health, Hospital Anxiety and Depression Scale for mental status, Global Rating of Change for treatment success and recurrence rate, and Mahomed Scale for the participant’s satisfaction. Adverse events will be recorded. Intention-to-treat analyses will be used. Ethics and dissemination Ethics committees of all clinical centres have approved this study. The leading centre is Shanghai Sixth People’s Hospital, whose approval number is 2021–153. New versions with appropriate amendments will be submitted to the committee for further approval. Final results will be published in peer-reviewed journals and presented at local, national and international conferences. Trial registration number ChiCTR2100050547.
    Type of Medium: Online Resource
    ISSN: 2044-6055 , 2044-6055
    URL: Article
    DOI: 10.1136/bmjopen-2021-057266
    Language: English
    Publisher: BMJ
    Publication Date: 2022
    detail.hit.zdb_id: 2599832-8
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Dual-engineered cartilage-targeting extracellular vesicles derived from mesenchymal stem cells enhance osteoarthritis treatment via miR-223/NLRP3/pyroptosis axis: Toward a precision therapy
    Elsevier BV ; 2023
    In:  Bioactive Materials Vol. 30 ( 2023-12), p. 169-183
    Details
    In: Bioactive Materials, Elsevier BV, Vol. 30 ( 2023-12), p. 169-183
    Type of Medium: Online Resource
    ISSN: 2452-199X
    URL: Article
    DOI: 10.1016/j.bioactmat.2023.06.012
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2970496-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Observation of novel topological states in hyperbolic lattices
    Springer Science and Business Media LLC ; 2022
    In:  Nature Communications Vol. 13, No. 1 ( 2022-05-26)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-05-26)
    Abstract: The discovery of novel topological states has served as a major branch in physics and material sciences. To date, most of the established topological states have been employed in Euclidean systems. Recently, the experimental realization of the hyperbolic lattice, which is the regular tessellation in non-Euclidean space with a constant negative curvature, has attracted much attention. Here, we demonstrate both in theory and experiment that exotic topological states can exist in engineered hyperbolic lattices with unique properties compared to their Euclidean counterparts. Based on the extended Haldane model, the boundary-dominated first-order Chern edge state with a nontrivial real-space Chern number is achieved. Furthermore, we show that the fractal-like midgap higher-order zero modes appear in deformed hyperbolic lattices, and the number of zero modes increases exponentially with the lattice size. These novel topological states are observed in designed hyperbolic circuit networks by measuring site-resolved impedance responses and dynamics of voltage packets. Our findings suggest a useful platform to study topological phases beyond Euclidean space, and may have potential applications in the field of high-efficient topological devices, such as topological lasers, with enhanced edge responses.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-022-30631-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2553671-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Retracted : Ras–ERK1/2 signaling promotes the development of uveal melanoma by downregulating H3K14ac
    Wiley ; 2019
    In:  Journal of Cellular Physiology Vol. 234, No. 9 ( 2019-09), p. 16011-16020
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 234, No. 9 ( 2019-09), p. 16011-16020
    Abstract: Ras–extracellular signal‐regulated protein kinases 1 and 2 (ERK1/2) signaling has been proposed as the crucial regulators in the development of various cancers. Histone acetylation at H3 lysine 14 (H3K14ac) is closely associated with gene expression and DNA damage. However, whether H3K14ac participates in mediating Ras–ERK1/2‐induced cell proliferation and migration in uveal melanoma cells remains unknown. The purpose of this study is to investigate the effect of H3K14ac on Ras–ERK1/2 affected uveal melanoma cell phenotypes. MP65 cells were transfected with Ras WT and Ras G12V/T35S , the unloaded plasmid of pEGFP‐N1 served as a negative control. Protein levels of phosphorylated ERK1/2 Thr202 and H3K14ac were assessed by western blot assay. Cell viability, number of colonies, migration, and the downstream genes of ERK1/2 were analyzed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide, soft‐agar colony formation, transwell, and chromatin immunoprecipitation assays. HA‐tag vectors of CLR3 and TIP60 and the small interfering RNAs that specific for CLR3 and MDM2 were transfected into MP65 cells to uncover the effects of CLR3, TIP60, and MDM2 on Ras–ERK1/2 mediated H3K14ac expression and MP65 cell phenotypes. We found that, Ras–ERK1/2 decreased H3K14ac expression in MP65 cells, and H3K14ac significantly suppressed Ras–ERK1/2‐induced cell viability, colony formation, and migration in MP65 cells. Moreover, the transcription of CYR61 , IGFBP3 , WNT16B , NT5E , GDF15 , and CARD16 was regulated by H3K14ac. Additionally, CLR3 silence recovered H3K14ac expression and reversed the effect of Ras–ERK1/2 on MP65 cell proliferation, migration and the mRNAs of ERK1/2 downstream genes. Besides, Ras–ERK1/2 decreased H3K14ac expression by MDM2‐mediated TIP60 degradation. In conclusion, Ras–ERK1/2 promoted uveal melanoma cells growth and migration by downregulating H3K14ac via MDM2‐mediated TIP60 degradation.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v234.9
    DOI: 10.1002/jcp.28259
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478143-8
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Dual-Engineered Cartilage-Targeting Extracellular Vesicles Derived from Mesenchymal Stem Cells Enhance Osteoarthritis Treatment Via miR-223/NLRP3/Pyroptosis Axis: Toward a Precision Therapy
    Elsevier BV ; 2022
    In:  SSRN Electronic Journal
    Details
    In: SSRN Electronic Journal, Elsevier BV
    Type of Medium: Online Resource
    ISSN: 1556-5068
    URL: Article
    DOI: 10.2139/ssrn.4290526
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Praseodymia–titania mixed oxide supported gold as efficient water gas shift catalyst: modulated by the mixing ratio of oxides
    Royal Society of Chemistry (RSC) ; 2022
    In:  RSC Advances Vol. 12, No. 9 ( 2022), p. 5374-5385
    Details
    In: RSC Advances, Royal Society of Chemistry (RSC), Vol. 12, No. 9 ( 2022), p. 5374-5385
    Abstract: Modulating the active sites for controllable tuning of the catalytic activity has been the goal of much research, however, this remains challenging. The O vacancy is well known as an active site in reducible oxides. To modify the activity of O vacancies in praseodymia, we synthesized a series of praseodymia–titania mixed oxides. Varying the Pr : Ti mole ratio (2 : 1, 1 : 2, 1 : 1, 1 : 4) allows us to control the electronic interactions between Au, Pr and Ti cations and the local chemical environment of the O vacancies. These effects have been studied study by X-ray photoelectron spectroscopy (XPS), CO diffuse reflectance Fourier transform infrared spectroscopy (CO-DRIFTS) and temperature-programmed reduction (CO-TPR, H 2 -TPR). The water gas shift reaction (WGSR) was used as a benchmark reaction to test the catalytic performance of different praseodymia–titania supported Au. Among them, Au/Pr 1 Ti 2 O x was identified to exhibit the highest activity, with a CO conversion of 75% at 300 °C, which is about 3.7 times that of Au/TiO 2 and Au/PrO x . The Au/Pr 1 Ti 2 O x also exhibited excellent stability, with the conversion after 40 h time-on-stream at 300 °C still being 67%. An optimal ratio of Pr content (Pr : Ti 1 : 2) is necessary for improving the surface oxygen mobility and oxygen exchange capability, a higher Pr content leads to more O vacancies, however with lower activity. This study presents a new route for modulating the active defect sites in mixed oxides which could also be extended to other heterogeneous catalysis systems.
    Type of Medium: Online Resource
    ISSN: 2046-2069
    URL: Article
    DOI: 10.1039/D1RA08572G
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2022
    detail.hit.zdb_id: 2623224-8
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Observation of interaction-induced phenomena of relativistic quantum mechanics
    Springer Science and Business Media LLC ; 2021
    In:  Communications Physics Vol. 4, No. 1 ( 2021-11-25)
    Details
    In: Communications Physics, Springer Science and Business Media LLC, Vol. 4, No. 1 ( 2021-11-25)
    Abstract: Relativistic quantum mechanics has been developed for nearly a century to characterize the high-energy physics in quantum domain, and various intriguing phenomena without low-energy counterparts have been revealed. Recently, with the discovery of Dirac cone in graphene, quantum materials and their classical analogies provide the second approach to exhibit the relativistic wave equation, making large amounts of theoretical predications become reality in the lab. Here, we experimentally demonstrate a third way to get into the relativistic physics. Based on the extended one-dimensional Bose-Hubbard model, we show that two strongly correlated bosons can exhibit Dirac-like phenomena, including the Zitterbewegung and Klein tunneling, in the presence of giant on-site and nearest-neighbor interactions. By mapping eigenstates of two correlated bosons to modes of designed circuit lattices, the interaction-induced Zitterbewegung and Klein tunneling are verified by measuring the voltage dynamics. Our finding not only demonstrates a way to exhibit the relativistic physics, but also provides a flexible platform to further investigate many interesting phenomena related to the particle interaction in experiments.
    Type of Medium: Online Resource
    ISSN: 2399-3650
    URL: Article
    DOI: 10.1038/s42005-021-00752-8
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2921913-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Retracted : Ras‐PI3K‐AKT signaling promotes the occurrence and development of uveal melanoma by downregulating H3K56ac expression
    Wiley ; 2019
    In:  Journal of Cellular Physiology Vol. 234, No. 9 ( 2019-09), p. 16032-16042
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 234, No. 9 ( 2019-09), p. 16032-16042
    Abstract: Uveal melanoma (UM) is an intraocular malignant tumor characterized by rapid progression and recurrence. The current conventional treatments are unsatisfactory. Histone acetylation at H3 lysine 56 (H3K56ac) has been reported to be a tumor suppressor in breast cancer. However, whether H3K56ac prevents the occurrence and development of UM remains uninvestigated. The study aimed to explore the regulatory effect of H3K56ac on Ras‐PI3K‐AKT induced UM cells proliferation and migration. Methods The vectors of pEGFP‐Ras WT , pEGFP‐K‐Ras G12V/Y40C , and pEGFP‐N1 were transfected into MP46 cells, and protein levels of phosphorylated AKT Ser473 and H3K56ac were examined using western blot analysis. The effect of H3K56ac on cell proliferation and migration were studied using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5 diphenyl tetrazolium bromide, colony formation, and Transwell assays. Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and chromatin immunoprecipitation assays were performed to determine the phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) downstream genes. Further, the regulatory effects of silent mating type information regulation 2 homolog‐1 (SIRT1), general control nonderepressible 5 (GCN5), and mouse double minute 2 homolog (MDM2) on Ras‐PI3K‐AKT affected H3K56ac expression were also investigated. Results H3K56ac expression was specifically downregulated by Ras‐PI3K‐AKT activation pathway. H3K56ac inhibited the tumorigenic effect of Ras‐PI3K‐AKT on MP46 cells viability, colony formation, and migration, as well as participated in regulating the transcription of PI3K/AKT downstream genes. SIRT1 silence recovered H3K56ac expression, and reversed the tumorigenic effect of Ras‐PI3K‐AKT activation on MP46 cells. Downregulation of H3K56ac induced by Ras‐PI3K‐AKT activation was found to be associated with MDM2‐mediated the degradation of GCN5. Conclusions The results demonstrated that Ras‐PI3K‐AKT signaling promoted UM cells proliferation and migration via downregulation of H3K56ac expression, which might be related to MDM2‐mediated the degradation of GCN5.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v234.9
    DOI: 10.1002/jcp.28261
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478143-8
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...