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  • 1
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] FIG. 1 Developmental northern blots showing the transcripts detected by cDNA clones of cyclins A (a) or B (b). Poly (A+) RNA from the following developmental stages was analysed: lane 1, 0-2 h embryos; lane 2, 2-4 h embryos; lane 3, first instar larvae; lane 4, second instar larvae; lane 5, third ...
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  • 2
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The two types of Drosophila melanogaster rDNA insertion occur at different sites in the 28S gene, separated by 51 nucleotide pairs. A short segment of rDNA is deleted at the site of the 5 kilobase type I insertion, whereas there is no such deletion of rDNA sequences which flank the type II ...
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 355 (1992), S. 253-255 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] FIG. 1 Anatomy of the outer proliferative centre, and origins of S-phase LPCs. a, b, S-phase cells in the third instar larval CNS visualized by Bromouridine deoxyribose (BUdR) incorporation (shown in green). Propidium iodide-stained nuclei shown in grey tones. Confocal images showing ...
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 363 (1993), S. 637-640 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Measurements of kinase activity in relation to the mitotic cycle have not yet been described in Drosophila owing to lack of an efficient protocol for synchronizing cultured Drosophila cells and an inability to achieve synchronous development of popula-tions of embryos. But as the first 13 mitoses ...
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  • 5
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] One of the unanswered questions in mammalian development is how the embryonic–abembryonic axis of the blastocyst is first established. It is possible that the first cleavage division contributes to this process, because in most mouse embryos the progeny of one two-cell blastomere ...
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  • 6
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Hiiragi et al. compare our model of the developing mouse egg with theirs. They seem to present patterning as equivalent to determination, but this is confusing as patterning does not have to mean determination. We have never stated that mouse embryo development is determined. Mouse ...
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  • 7
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We have examined the dynamics of the localisation of the polo-like kinase 1 (Plk1) during maturation of the mouse oocyte. Levels of Plk1 protein increase following germinal vesicle breakdown, at which time the enzyme begins to accumulate at discrete positions on the condensing chromosomes and, subsequently, at the poles of the meiotic spindle, which moves towards the cortex of the egg. Interestingly, at metaphase in both meiotic divisions, Plk1 shows a punctate localisation along the broad spindle poles. Moreover, the punctate distribution of Plk1 on the meiotic chromosomes appears at early anaphase to correspond to the centromeric regions. The protein relocates to the spindle midzone during late anaphase and then associates with the midbody at telophase. We have confirmed the specific pattern of immuno-localisation seen in fixed preparations by observing the distribution of Plk1 tagged with green fluorescent protein in living oocytes. We discuss the localisation of the enzyme in light of the structure of the spindle poles, which are known to lack centrioles, and the highly asymmetric nature of the meiotic divisions.
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  • 8
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract.  We describe genetic interactions between mutations in mgr, asp, and polo, genes required for the correct behaviour of the spindle poles in Drosophila. The phenotype of a polo 1 mgr double mutant is more similar to mgr than polo 1 , but the frequency of circular monopolar figures (CMFs) seen with either mutant alone is additive, suggesting that the two gene products are required for independent functions in the formation of bipolar spindles. The asp E3 mgr double mutant arrests much earlier in development than either mutant alone, indicative of a strong block to cell proliferation. We discuss whether the lack of microtubular structures in these cells reflects an extended mitotic arrest, or if it is a more direct consequence of the double mutant combination. A polo 1 asp E3 double mutant shows a dramatic synergistic increase in mitotic frequency. The loss of CMFs normally associated with the polo 1 phenotype suggests that the Asp microtubule-associated protein is required to maintain the structure of spindle poles. We speculate that Asp protein might be a substrate for the serine-threonine protein kinase encoded by polo.
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  • 9
    Publication Date: 2022-05-25
    Description: Author Posting. © Elsevier B.V., 2006. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Marine Systems 66 (2007): 173-181, doi:10.1016/j.jmarsys.2006.03.020.
    Description: Estimation of global and regional air–sea fluxes of climatically important gases is a key goal of current climate research programs. Gas transfer velocities needed to compute these fluxes can be estimated by combining altimeter-derived mean square slope with an empirical relation between transfer velocity and mean square slope derived from field measurements of gas fluxes and small-scale wave spectra [Frew, N.M., Bock, E.J., Schimpf, U., Hara, T., Hauβecker, H., Edson, J.B., McGillis, W.R., Nelson, R.K., McKenna, S.P., Uz, B.M., Jähne, B., 2004. Air–sea gas transfer: Its dependence on wind stress, small-scale roughness and surface films, J. Geophys. Res., 109, C08S17, doi: 10.1029/2003JC002131.]. We previously reported initial results from a dual-frequency (Ku- and C-band) altimeter algorithm [Glover, D.M., Frew, N.M., McCue, S.J., Bock, E.J., 2002. A Multi-year Time Series of Global Gas Transfer Velocity from the TOPEX Dual Frequency, Normalized Radar Backscatter Algorithm, In: Gas Transfer at Water Surfaces, editors: Donelan, M., Drennan, W., Saltzman, E., and Wanninkhof, R., Geophysical Monograph 127, American Geophysical Union, Washington, DC, 325–331.] for estimating the air–sea gas transfer velocity (k) from the mean square slope of short wind waves (40–100 rad/m) and derived a 6-year time series of global transfer velocities based on TOPEX observations. Since the launch of the follow-on altimeter Jason-1 in December 2001 and commencement of the TOPEX/Jason-1 Tandem Mission, we have extended this time series to 12 years, with improvements to the model parameters used in our algorithm and using the latest corrected data releases. The prospect of deriving multi-year and interdecadal time series of gas transfer velocity from TOPEX, Jason-1 and follow-on altimeter missions depends on precise intercalibration of the normalized backscatter. During the Tandem Mission collinear phase, both satellites followed identical orbits with a mere 73-s time separation. The resulting collocated, near-coincident normalized radar backscatter (σ°) data from both altimeters present a unique opportunity to intercalibrate the two instruments, compare derived fields of transfer velocity and estimate the precision of the algorithm. Initial results suggest that the monthly gas transfer velocity fields generated from the two altimeters are very similar. Comparison of along-track Ku-band and C-band σ° during the collinear phase indicates that observed discrepancies are due primarily to small offsets between TOPEX and Jason-1 σ°. The Jason-1 k values have an apparent bias of + 4% relative to TOPEX, while the precision estimated from the two observation sets is 5–7% and scales with k. The resultant long-term, global, mean k is 16 cm/h.
    Description: We gratefully acknowledge funding support from NASA under grant NAGW–2431 and JPL contract 961425.
    Keywords: Gas exchange ; Altimetry ; Mean square slope
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
    Format: application/pdf
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  • 10
    Publication Date: 2022-05-25
    Description: Presented at Articulating the Cyberinfrastructure Needs of the Ocean Ecosystem Dynamics Community, Biological and Chemical Oceanographic Data Management Office, Woods Hole, MA, 7 – 8 Oct 2013
    Description: An EarthCube Water Column Domain End-User Workshop hosted by the Biological and Chemical Oceanographic Data Management Office (BCO-DMO) was held October 7-8, 2013 at Woods Hole Oceanographic Institution. The goal of the workshop was to articulate cyberinfrastructure needs of the ocean ecosystem dynamics community with particular focus on the challenges presented by multi-disciplinary marine ecosystem research that requires investigations in four dimensions. The workshop included 50 participants in the domain of oceanic ecosystem dynamics (established and early career researchers, teaching faculty, graduate students, postdocs, data and information managers and cyber-related researchers) to explore and document the community’s cyberinfrastructure needs from the users’ viewpoint.
    Description: NSF #1338892
    Keywords: Cyberinfrastructure ; Ocean Ecosystem Dynamics ; EarthCube
    Repository Name: Woods Hole Open Access Server
    Type: Other
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