ISSN:
1432-2277
Keywords:
Preservation, liver endothelial cells
;
Liver endothelial cells, preservation
;
Endothelial cells, liver, preservation
;
Preservation solutions
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Previously, we demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution. Here, the effects of Histidine-Tryptophan-Ketoglutarate (HTK) and Euro-Collins (EC) solutions on these cells were studied. In HTK solution, 83%±4% of the cells had lost viability after 9 h of incubation at 4°C. The addition of cyanide (1 mM) to simulate hypoxic conditions protected the cells to the extent that only 9%±1% of the cells lost viability over the same period; the addition of glucose (10 mM) led to increased cell injury. ATP levels were highest in the incubations with the most rapid loss of viability. In Krebs-Henseleit buffer and EC solution, in contrast, cell injury increased upon addition of cyanide; the addition of glucose to Krebs-Henseleit buffer decreased injury. We conclude that the injury to cultured liver endothelial cells during cold incubation in HTK solution is energy-dependent, as it is in UW solution, whereas cells behave differently in EC solution and Krebs-Henseleit buffer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00337103
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