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  • 1
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; Ratoon ; Molecular marker ; QTL
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Although F2s are the most informative populations for genetic analysis, it has been difficult to use F2 populations directly for QTL analysis because it is usually difficult to assess the reliability of the data, due to an inability to estimate the experimental errors. In this study, we performed a QTL analysis for yield and yield-component traits of an F2 population based on data from replicated field trials over 2 years using vegetative shoots of ratooned plants, making use of the ratooning habit of rice. The objective of this study was to explore the possibility of conducting QTL analyses directly based on an F2 population by means of ratooning plants. The experimental population was from a cross between ’Zhenshan 97’ and ’Minghui 63’, the parents of ’Shanyou 63’, an elite rice hybrid widely grown in China. A genetic linkage map containing 151 molecular markers was constructed for QTL mapping. A total of 20 distinct QTLs were detected; eight of these were detected in both years and remaining 12 in only 1 year. Compared with the results of our previous analysis of the F2:3 families from the same cross, it was shown that most of the QTLs detected in the ratooned F2 population were also detected in the F2:3 population. However, the estimates of both additive and dominant types of genetic effects for many of the QTLs based on F2 ratoons were substantially larger than those based on F2:3 families. The results indicate that vegetatively ratooned F2 populations may have considerable utility in the mapping of QTLs, especially if dominant types of gene actions are of concern, although there were certain technical limitations in making use of such populations in the experiments.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; Genetic differentiation ; Classification of parental lines ; Restriction fragment length polymorphism (RFLP) ; Genic interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study was intended to investigate the extent of genetic differentiation in parental lines of rice hybrids and to analyze the genetic basis underlying the fertility phenomenon in distant crosses. Two subsets of rice material (111 entries in total) were used, including 81 doubled-haploid (DH) lines and 30 Indica and Japonica rice varieties or lines (as a control). The DH lines was derived from a heterotic Indica/Japonica cross (Gui630/02428) by anther culture. The materials in the control represent a broad spectrum of the Asian cultivated rice gene pool including landraces, primitive cultivars, historically important cultivars, modern elite cultivars, super rice and parents of superior hybrids. In accordance with the NC II design, 57 out of the DH lines were test-crossed to two important wide compatibility lines: photoperiod-sensitive genetic male sterile (PGMS) line N422s and thermo-sensitive genetic male sterile (TGMS) line Peiai64s. The F1s and their parents, 182 entries in total, were examined for the performance of seven traits in a replicated field trial. All the rice materials was surveyed for polymorphisms using 92 RFLP markers selected from two published molecular marker linkage maps. Genotypes of the F1 hybrids at the molecular-marker loci were deduced from the parental genotypes. The analysis showed that there were two types of genetic differentiation in the two subsets of rice material; that is, qualitative differentiation in the control and quantitative differentiation in the DH lines. In addition, favorable genic interactions (both intra- or inter-locus) contributed to better increase the fertility in hybrids of distant crosses through incorporation of a wide-compatibility line as the female parent. Favorable genic interactions can be applied in hybrid rice breeding programs by selecting parents with an appropriate extent of genetic differentiation.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 97 (1998), S. 407-412 
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; Indica-japonica cross ; Hybrid sterility ; Molecular marker ; Genetic analysis ; Epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wide-compatibility varieties (WCVs) are a special class of rice germplasm that is able to produce fertile hybrids when crossed to both indica and japonica rice varieties. WCVs may differ greatly in their spectrum and level of compatibility. The objective of this study was to determine the genetic basis of wide-compatibility conferred by ‘Dular’, a landrace variety from India that has demonstrated a high level of wide-compatibility in previous studies with a broad range of indica and japonica varieties. A three-way cross (‘Balilla/Dular//Nanjing 11’) was made and the resulting F1 population evaluated in the field for spikelet fertility. A total of 235 plants from this population was assayed individually for restriction fragment length polymorphisms (RFLPs) at 159 marker loci covering the entire rice genome at regular intervals. Quantitative trait locus (QTL) analysis identified 5 loci, located on chromosomes 1, 3, 5, 6 and 8, as having significant effects on hybrid fertility, which jointly explained 55.5% of the fertility variation in this population. The QTL on chromosome 5 ( f5) showed the largest effect on hybrid fertility, followed by those on chromosomes 6 ( f6), 3 ( f3) and 1 ( f1), with the one on chromosome 8 ( f8) having the smallest effect. Genotypes each composed of an allele from ‘Dular’ and an allele from ‘Nanjing 11’ at four ( f3, f5, f6 and f8) of the five QTLs contributed to the increase of fertility in the population. In contrast, the genotype composed of alleles from ‘Balilla’ and ‘Nanjing 11’ at the fifth locus ( f1) was in the direction of increasing fertility. Analysis of variance using marker genotypes at the five QTLs as the groups detected two interactions involving four of the five loci, a 2-locus interaction between f5 and f8 and a 3-locus interaction among f3, f5 and f6. The level of hybrid fertility is the result of complex interactions among these loci. The implication of the present findings in the utilization of the wide-compatibility of ‘Dular’ in rice breeding programs is also discussed.
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  • 4
    Publication Date: 2011-07-15
    Description: Author(s): Y. D. Fang, Y. H. Zhang, X. H. Zhou, M. L. Liu, J. G. Wang, Y. X. Guo, X. G. Lei, W. Hua, F. Ma, S. C. Wang, B. S. Gao, S. C. Li, X. L. Yan, L. He, Z. G. Wang, F. Fang, X. G. Wu, C. Y. He, Y. Zheng, Z. M. Wang, Y. Shi, and F. R. Xu Excited states of the stable nucleus 195 Pt have been studied using an in-beam γ -ray spectroscopic technique following the incomplete fusion of 7 Li on an 192 Os target at 44 MeV. A level scheme built on the I π =13/2 + isomer has been established up to I π =33/2 − at an excitation energy of about 2.6 MeV. S... [Phys. Rev. C 84, 017301] Published Thu Jul 14, 2011
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
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  • 5
    Publication Date: 2012-11-17
    Description: Author(s): B. S. Gao, X. H. Zhou, Y. D. Fang, Y. H. Zhang, S. C. Wang, N. T. Zhang, M. L. Liu, J. G. Wang, F. Ma, Y. X. Guo, X. G. Wu, C. Y. He, Y. Zheng, Z. M. Wang, S. C. Li, X. L. Yan, L. He, Z. G. Wang, Y. Zheng, F. Fang, and X. M. Chen High-spin states in the odd-odd 194 Au nucleus have been investigated using the 192 Os( 7 Li,5 n ) reaction at a beam energy of 44 MeV. The previously known structures built on the π h 11/2 −1 ⊗ ν i 13/2 −1 and π h 11/2 −1 ⊗ ν i 13/2 −2 ν j configurations are extended to high-spin states, and a new I π = (20 + ) state associat... [Phys. Rev. C 86, 054310] Published Fri Nov 16, 2012
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
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  • 6
    Publication Date: 2012-11-20
    Description: Author(s): C. B. Li, X. G. Wu, X. F. Li, C. Y. He, Y. Zheng, G. S. Li, S. H. Yao, S. P. Hu, H. W. Li, J. L. Wang, J. J. Liu, C. Xu, J. J. Sun, and W. W. Qu The lifetime of the 2 1 + state in 174 Os was measured in a fast timing experiment using LaBr 3 (Ce) crystals for γ -ray detection. The excellent energy resolution in conjunction with the superb time properties of the new material allows for the reliable handling of the background, thus providing very pre... [Phys. Rev. C 86, 057303] Published Mon Nov 19, 2012
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
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  • 7
    Publication Date: 2012-02-07
    Description: Author(s): S. C. Wang, X. H. Zhou, Y. D. Fang, Y. H. Zhang, N. T. Zhang, B. S. Gao, M. L. Liu, J. G. Wang, F. Ma, Y. X. Guo, S. C. Li, X. L. Yan, L. He, Z. G. Wang, F. Fang, X. G. Wu, C. Y. He, Y. Zheng, Z. M. Wang, G. X. Dong, and F. R. Xu Excited states in 195 Au have been studied experimentally via the 192 Os( 7 Li, 4 n ) reaction at a beam energy of 44 MeV. Based on the γ - γ - t coincidence measurement, a level scheme consisting of 15 new transitions and 10 new levels is established for 195 Au. The triaxial shape-polarizing effect of the hig... [Phys. Rev. C 85, 027301] Published Mon Feb 06, 2012
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
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  • 8
    Publication Date: 2011-05-27
    Description: Author(s): Y. D. Fang, Y. Shi, Y. H. Zhang, B. S. Gao, S. C. Wang, X. H. Zhou, M. L. Liu, J. G. Wang, F. Ma, Y. X. Guo, X. G. Wu, C. Y. He, Y. Zheng, Z. M. Wang, S. C. Li, X. L. Yan, L. He, Z. G. Wang, F. Fang, and F. R. Xu Excited states in the stable nucleus 193 Ir have been investigated through an in-beam γ -ray spectroscopic technique following the 192 Os ( 7 Li , α 2 n ) reaction at a beam energy of 44 MeV. A level scheme built on the π h 11/2 isomer has been extended to high-spin states using eleven newly observed γ transiti... [Phys. Rev. C 83, 054323] Published Thu May 26, 2011
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
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