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  • Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; CTD, Sea-Bird SBE 911plus; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hata_etal_02; LICOR quantameter (LI-COR LI-192SA); North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Precision analyzer (Model EN-501A, Kimoto Electric); Radiation, photosynthetically active; Rocky-shore community; Salinity; Salinometer, inductive; Shiraho coral reef; Temperate; Temperature, water  (1)
  • Chinese hamster ovary (CHO) cells  (1)
  • ascorbic acid  (1)
Document type
Keywords
  • Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; CTD, Sea-Bird SBE 911plus; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hata_etal_02; LICOR quantameter (LI-COR LI-192SA); North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Precision analyzer (Model EN-501A, Kimoto Electric); Radiation, photosynthetically active; Rocky-shore community; Salinity; Salinometer, inductive; Shiraho coral reef; Temperate; Temperature, water  (1)
  • Chinese hamster ovary (CHO) cells  (1)
  • ascorbic acid  (1)
  • Rubisco activase  (1)
  • choline  (1)
  • +
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  • 1
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    Unknown
    PANGAEA
    In:  Supplement to: Hata, Hiroshi; Kudo, Setsuko; Yamano, Hiroya; Kurano, Norihide; Kayanne, Hajime (2002): Organic carbon flux in Shiraho coral reef (Ishigaki Island, Japan). Marine Ecology Progress Series, 232, 129-140, https://doi.org/10.3354/meps232129
    Publication Date: 2024-03-15
    Description: Organic carbon flux and community production rates were estimated on Shiraho coral reef (Ishigaki Island, Japan) from 19 to 26 September 1998. The daily net community production (Pn) and respiration rate (R) during the study period were 3 to 79 and 596 mmolC m-2 d-1, respectively. This resulted in a daily gross community production (Pg) of 599 to 675 mmolC m-2 d-1. The variation of Pn associated with the uncertainty of the curve fitting parameters of light response curves for photosynthesis was estimated using an error propagation formula. The averaged Pn ± SE was 36 ± 12 mmolC m-2 d-1 (n = 23), indicating that the Pn was significantly positive (t-test, p 〈 0.05). The apparent fluxes of dissolved organic carbon (DOC) and particulate organic carbon (POC) on the reef were estimated as 30 to 36 and 5 to 7 mmolC m-2 d-1, respectively; The sum of which was comparable with the Pn during the study period. The sediment trap study conducted at 1 km off the reef and 40 m depth showed that the vertical flux of POC was 1.0 mmolC m-2 d-1. The results indicated that 6 to 7 % of the Pg was exported to offshore and about 14 to 20% of the POC exported from the reef flat and 0.2% of the Pg reached 1 km off and 40 m depth.
    Keywords: Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; CTD, Sea-Bird SBE 911plus; Entire community; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; EXP; Experiment; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hata_etal_02; LICOR quantameter (LI-COR LI-192SA); North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Precision analyzer (Model EN-501A, Kimoto Electric); Radiation, photosynthetically active; Rocky-shore community; Salinity; Salinometer, inductive; Shiraho coral reef; Temperate; Temperature, water
    Type: Dataset
    Format: text/tab-separated-values, 308 data points
    Location Call Number Limitation Availability
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  • 2
    ISSN: 1573-0778
    Keywords: Chinese hamster ovary (CHO) cells ; vitamins ; ascorbic acid ; choline ; nicotinamide ; thiamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were α-modified Eagle's minimum essential medium (MEM-α) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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