Publication Date:
2024-03-15
Description:
The effects of ocean acidification on the life-cycle stages of the coccolithophore Emiliania huxleyi and their by light were examined. Calcifying diploid and noncalcifying haploid cells (Roscoff culture collection 1216 and 1217) were acclimated to present-day and elevated CO2 partial pressures (PCO2; 38.5 vs. 101.3 Pa, ., 380 vs. 1000 matm) under low and high light (50 vs. 300 mmol photons m-2 s-1). Growth rates as well as quotas and production rates of C and N were measured. Sources of inorganic C for biomass buildup were using a 14C disequilibrium assay. Photosynthetic O2 evolution was measured as a function of dissolved inorganic C and light by means of membrane-inlet mass spectrometry. The diploid stage responded to elevated PCO2 by shunting resources from the production of particulate inorganic C toward organic C yet keeping the production of total particulate C constant. As the effect of ocean acidification was stronger under low light, the diploid stage might be less affected by increased acidity when energy availability is high. The haploid stage maintained elemental composition and production rates under elevated PCO2. Although both life-cycle stages involve different ways of dealing with elevated PCO2, the responses were generally modulated by energy availability, being typically most pronounced under low light. Additionally, PCO2 responses resembled those induced by high irradiances, indicating that ocean acidification affects the interplay between energy-generating processes (photosynthetic light reactions) and processes competing for energy (biomass buildup and calcification). A conceptual model is put forward explaining why the magnitude of single responses is determined by energy availability.
Keywords:
Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Auto-analyzer, Technicon Traacs 800; Bicarbonate ion; Bicarbonate ion, standard deviation; Bicarbonate uptake/net fixation ratio; Bicarbonate uptake/net fixation ratio, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcification/Dissolution; Calcite saturation state; Calcite saturation state, standard deviation; Calculated; Calculated, see reference(s); Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, inorganic, particulate, per cell; Carbon, organic, particulate, per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a/particulate organic carbon ratio, standard deviation; Chlorophyll a per cell; Chromista; Emiliania huxleyi; EPOCA; Estimated; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Haptophyta; Laboratory experiment; Laboratory strains; Light; Light:Dark cycle; Mass spectrometer SL 20-20 (SerCon); Nitrate; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon, production, standard deviation; Particulate inorganic carbon/particulate organic carbon ratio; Particulate inorganic carbon/particulate organic carbon ratio, standard deviation; Particulate inorganic carbon per cell, standard deviation; Particulate inorganic carbon production per cell; Particulate organic carbon, production, standard deviation; Particulate organic carbon content per cell, standard deviation; Particulate organic carbon production per cell; Particulate organic nitrogen per cell; Particulate organic nitrogen per cell, standard deviation; Particulate organic nitrogen production, standard deviation; Pelagos; pH; pH, standard deviation; pH meter, WTW, pH 3000; Phosphate; Phytoplankton; Pigments, Turner fluorometer; Primary production/Photosynthesis; Production of particulate organic nitrogen; Radiation, photosynthetically active; Salinity; Single species; Species; Temperature, water; Total particulate carbon production, standard deviation; Total particulate carbon production per cell
Type:
Dataset
Format:
text/tab-separated-values, 536 data points
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