Publication Date:
2022-05-25
Description:
Author Posting. © Springer, 2008. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Extremophiles 12 (2008): 627-640, doi:10.1007/s00792-008-0167-5.
Description:
The bacterial and archaeal communities of three deep-sea hydrothermal vent systems
located on the Mid-Atlantic Ridge (MAR; Rainbow, Logatchev and Broken Spur) were
investigated using an integrated culture-dependent and independent approach.
Comparative molecular phylogenetic analyses, using the 16S rRNA gene and the deduced
amino acid sequences of the alpha and beta subunits of the ATP citrate lyase encoding
genes were carried out on natural microbial communities, on an enrichment culture
obtained from the Broken Spur chimney, and on novel chemolithoautotrophic bacteria
and reference strains originally isolated from several different deep-sea vents. Our data
showed that the three MAR hydrothermal vent chimneys investigated in this study host
very different microbial assemblages. The microbial community of the Rainbow chimney
was dominated by thermophilic, autotrophic, hydrogen-oxidizing, sulfur- and nitrate
reducing Epsilonproteobacteria related to the genus Caminibacter. The detection of
sequences related to sulfur-reducing bacteria and archaea (Archaeoglobus) indicated that
thermophilic sulfate reduction might also be occurring at this site. The Logatchev
bacterial community included several sequences related to mesophilic sulfur-oxidizing
bacteria, while the archaeal component of this chimney was dominated by sequences
related to the ANME-2 lineage, suggesting that anaerobic oxidation of methane may be
occurring at this site. Comparative analyses of the ATP citrate lyase encoding genes from
natural microbial communities suggested that Epsilonproteobacteria were the dominant
primary producers using the reverse TCA cycle (rTCA) at Rainbow, while Aquificales of
the genera Desulfurobacterium and Persephonella were prevalent in the Broken Spur
chimney.
Description:
This research was supported by NSF grants MCB 04-56676 (C.V.), OCE 03-27353 (C.V.), MCB 04-56689 (S.M.S.), a grant from the New Jersey Agricultural Experiment Station to C.V., and a NIH Ph.D. Training
Program in Biotechnology Fellowship (NIH NIGMS 5 T32 GM08339) to J.V. M.H. was
supported through a postdoctoral scholarship from the Woods Hole Oceanographic
Institution.
Keywords:
Epsilonproteobacteria
;
Aquificales
;
ANME
;
ATP citrate lyase
;
Black smoker
;
Deep-sea vent
;
rTCA cycle
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
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