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  • Ac  (2)
  • 2,4-dichlorophenoxyacetic acid (2,4-D)  (1)
  • Somaclonal variation  (1)
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  • 1
    ISSN: 1573-5028
    Keywords: 2,4-dichlorophenoxyacetic acid (2,4-D) ; detoxification ; herbicide resistance ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plants resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) were produced through the genetic engineering of a novel detoxification pathway into the cells of a species normally sensitive to 2,4-D. We cloned the gene for 2,4-D monooxygenase, the first enzyme in the plasmid-encoded 2,4-D degradative pathway of the bacterium Alcaligenes eutrophus, into a cauliflower mosaic virus 35S promoter expression vector and introduced it into tobacco plants by Agrobacterium-mediated transformation. Transgenic tobacco plants expressing the highest levels of the monooxygenase enzyme exhibited increased tolerance to 2,4-D in leaf disc and seed germination assays, and young plants survived spraying with levels of herbicide up to eight times the usual field application rate. The introduction of the gene for 2,4-D monooxygenase into broad-leaved crop plants, such as cotton, should eventually allow 2,4-D to be used as an inexpensive post-emergence herbicide on economically important dicot crops.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: Ac ; transposition ; progeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To assess the potential of the maize transposable element Ac for gene tagging in heterologous plant species we monitored transcription, excision and transposition of the element in transgenic tobacco plants and their selfed progeny. Ac excised in the majority of primary regenerants and continued to excise in the first-generation progeny plants. In one primary regenerant Ac was transcribed but did not excise. Fourteen of eighteen kanamycin-resistant progeny from this plant showed Ac excision, suggesting that excision of Ac may have been activated during meiosis or in embryo development. This finding, together with the more general observation of continued Ac mobility in the progeny of transformed plants in which Ac had excised, suggests that Ac will be useful for gene tagging.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 202 (1986), S. 235-239 
    ISSN: 1617-4623
    Keywords: Maize ; Alcohol dehydrogenase ; Somaclonal variation ; Mutation ; Gene sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants regenerated from tissue cultures of maize were screened for variants of ADH1 and ADH2. Root extracts of 645 primary regenerant plants were tested, and one stable mutant of Adh1 was detected. The mutant gene (Adh1-Usv) produces a functional enzyme with a slower electrophoretic mobility than that of the progenitor Adh1-S allele, and is stably transmitted to progeny. The mutant was not present among four other plants derived from the same immature embryo, and therefore arose as a consequence of the culture procedure. The gene of Adh1-Usv was cloned and sequenced. A single base change in exon 6 was the only alteration found in the gene sequence. This would translate in the polypeptide sequence to a valine residue substituting for a glutamic acid residue, resulting in the loss of a negative charge and the production of a protein with slower electrophoretic mobility.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1617-4623
    Keywords: Ac ; Transposable element ; Maize ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A combination of cDNA cloning and S1 analyses of RNA isolated from maize seedlings that carry an active Ac element has been used to define the Ac transcript. The primary transcript contains 4 introns that are excised to give a processed message which we predict to be approximately 3.4 kb. There are a number of transcription initiation sites clustered within a 90 base region about 300 bases from one end of the element. The first ATG is 600–690 bases from the transcription start and precedes an open reading frame of 807 amino acids— the putative transposase. The transcript extends to within 261 bases from the other end of the element. S1 analysis of RNa from a transgenic tobacco plant carrying an intact copy of the Ac element demonstrated a transcript identical to that in maize, although the preferred initiation sites differ.
    Type of Medium: Electronic Resource
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