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  • 13C carbon fixation; 2L-photobioreactors; Alkalinity, total; Autoanalyzer-Colorimeter; Calculated using CO2SYS; Carbon, inorganic, dissolved; Carbon capture and storage; CCS; CO2 leakage; Code; Continuous-flow IRMS Europa 20-20 with ANCA TG trace gas separation module; culture experiment; diatoms; Electrochemical probe; Experiment; Flow-cytometer; IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples; Light microscope; Medium; Nitrogen, inorganic, dissolved; pH; Phosphate; Phytoplankton; Phytoplankton, δ13C, particulate organic carbon; Potentiometric open-cell titration; Prokaryotes; Prokaryotes, δ13C, particulate organic carbon; Thalassiosira rotula; Thalassiosira rotula, standard deviation; Time in hours; δ13C, dissolved inorganic carbon  (1)
  • 14C in-situ incubation; 3H-leucine incorporation; Alkalinity, total; Ammonium; Autoanalyzer-Colorimeter; Bottle, Niskin 5-L; Carbon, organic, dissolved; Carbon, organic, particulate; Chlorophyll a; Colorimetric; Conductivity; CTD, SEA-BIRD SBE 911plus, SN 4380; DATE/TIME; Depth, bathymetric; DEPTH, water; Depth of Secchi Disk; Event label; Gravimetric analysis; High Temperature Combustion gas chromatograph separation; LATITUDE; LONGITUDE; Mediterranean Sea Acidification in a Changing Climate; MedSeA; MedSeA_Z1; MedSeA_Z2; MedSeA_Z3; MedSeA_Z4; MedSeA_Z5; MedSeA_Z7; MedSeA_Z8; NIS_5L; Nitrate; Nitrite; Nitrogen, total, particulate, salt corrected; Nitrogen, total dissolved; Oxygen; pH; Phaeopigments; Phosphate; Phosphorus, particulate; Phosphorus, total dissolved; Potentiometric open-cell titration; Primary production of carbon; Primary production of carbon, standard deviation; Profiling Natural Fluorometer PNF-300A; Prokaryotic carbon production; Prokaryotic carbon production, standard deviation; Radiation, photosynthetically active; Salinity; Silicic acid; Spectrofluorometry; Station label; Strain gauge; Suspended matter, total; Temperature, water; Titration, Winkler; Visual observation; Wet-oxidation Autoanalyzer-Colorimeter  (1)
  • Ammonium; Carbon, inorganic, particulate; Carbon, organic, particulate; Cell; DATE/TIME; Day of experiment; ECO2; Length; Nitrate; Nitrite; Nitrogen, total, particulate; pH; pH meter; Phosphate; Prokaryotes, heterotroph; Silicon dioxide; Species; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; Treatment; Width  (1)
Document type
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  • 1
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    Culture experiments to evaluate CO2 effects on growth and morphology of the coccolithophore Pleurochrysis cf. pseudoroscoffensis (2014)
    PANGAEA
    In:  Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico
    Details
    Publication Date: 2023-05-27
    Description: In the framework of the Italian project CO2 Monitor, two culture experiments were carried out in vertical closed photobioreactors with Pleurochrysis cf. pseudoroscoffensis Gayral & Fresnel 1983, a coccolithophore isolated from the Gulf of Trieste (North Adriatic Sea). The aim of this study was to investigate the effects induced by pH variations due to CO2 emissions on its growth and morphology. Two experiments were carried out with two different CO2 concentrations (1 and 2%) relatively to the total volume of insufflated air. Growth and cell size in light microscopy, morphology and coccolith size in scanning electron microscopy, particulate nitrogen (PN) and particulate inorganic and organic carbon (PIC and POC) content of the coccolithophore were investigated during the light and dark phases. Dissolved inorganic nutrient (nitrate and phosphate) concentrations and pH of the medium and the presence of heterotrophic prokaryotes (HP) were monitored as well.
    Keywords: Ammonium; Carbon, inorganic, particulate; Carbon, organic, particulate; Cell; DATE/TIME; Day of experiment; ECO2; Length; Nitrate; Nitrite; Nitrogen, total, particulate; pH; pH meter; Phosphate; Prokaryotes, heterotroph; Silicon dioxide; Species; Sub-seabed CO2 Storage: Impact on Marine Ecosystems; Treatment; Width
    Type: Dataset
    Format: text/tab-separated-values, 750 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 2
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    Thalassiosira rotula culture experiments for the assessment of phytoplankton stable carbon isotopic composition as a tool to monitor anthropogenic CO2 submarine leakages (2020)
    PANGAEA
    Details
    Publication Date: 2024-02-16
    Description: Three culture experiment on the diatom Thalassiosira rotula were conducted within two 2L-photobioreactors under controlled conditions of light and temperature (20 ± 0.02 °C). During each experiment, diatoms were simultaneously grown in the two bioreactors filled respectively with different media. In one of the two bioreactor microalgae were grown in a natural seawater (NAT) medium, whereas in the second one an artificial seawater medium (ASW) was used. ASW medium was artificially prepared, to reach a modified isotopic composition of DIC using an industrial compressed CO2, and at each experiment a different initial DIC concentration was tested to verify if carbon availability would affect phytoplankton algal metabolism. For NAT medium, a sterile-filtered Adriatic Sea water was used and a natural DIC concentration used. During the experiments, the evolution of T. rotula abundance was investigated, together with the variation of the isotopic composition of dissolved inorganic carbon, phytoplankton organic carbon (as particulate organic carbon) and prokaryotes organic carbon (as the fraction 〈 10 µm of particulate organic carbon). Carbonate system parameters (in situ pHNBS, total alkalinity, DIC and dissolved CO2 concentration), nutrient (dissolved inorganic nitrogen and phosphate) concentration and prokaryotes abundance were also analysed during diatom cultivation.
    Keywords: 13C carbon fixation; 2L-photobioreactors; Alkalinity, total; Autoanalyzer-Colorimeter; Calculated using CO2SYS; Carbon, inorganic, dissolved; Carbon capture and storage; CCS; CO2 leakage; Code; Continuous-flow IRMS Europa 20-20 with ANCA TG trace gas separation module; culture experiment; diatoms; Electrochemical probe; Experiment; Flow-cytometer; IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples; Light microscope; Medium; Nitrogen, inorganic, dissolved; pH; Phosphate; Phytoplankton; Phytoplankton, δ13C, particulate organic carbon; Potentiometric open-cell titration; Prokaryotes; Prokaryotes, δ13C, particulate organic carbon; Thalassiosira rotula; Thalassiosira rotula, standard deviation; Time in hours; δ13C, dissolved inorganic carbon
    Type: Dataset
    Format: text/tab-separated-values, 433 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 3
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    Biogeochemical variations in the gulf of Trieste, Italy (2011-2013) (2015)
    PANGAEA
    In:  Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico
    Details
    Publication Date: 2024-04-20
    Keywords: 14C in-situ incubation; 3H-leucine incorporation; Alkalinity, total; Ammonium; Autoanalyzer-Colorimeter; Bottle, Niskin 5-L; Carbon, organic, dissolved; Carbon, organic, particulate; Chlorophyll a; Colorimetric; Conductivity; CTD, SEA-BIRD SBE 911plus, SN 4380; DATE/TIME; Depth, bathymetric; DEPTH, water; Depth of Secchi Disk; Event label; Gravimetric analysis; High Temperature Combustion gas chromatograph separation; LATITUDE; LONGITUDE; Mediterranean Sea Acidification in a Changing Climate; MedSeA; MedSeA_Z1; MedSeA_Z2; MedSeA_Z3; MedSeA_Z4; MedSeA_Z5; MedSeA_Z7; MedSeA_Z8; NIS_5L; Nitrate; Nitrite; Nitrogen, total, particulate, salt corrected; Nitrogen, total dissolved; Oxygen; pH; Phaeopigments; Phosphate; Phosphorus, particulate; Phosphorus, total dissolved; Potentiometric open-cell titration; Primary production of carbon; Primary production of carbon, standard deviation; Profiling Natural Fluorometer PNF-300A; Prokaryotic carbon production; Prokaryotic carbon production, standard deviation; Radiation, photosynthetically active; Salinity; Silicic acid; Spectrofluorometry; Station label; Strain gauge; Suspended matter, total; Temperature, water; Titration, Winkler; Visual observation; Wet-oxidation Autoanalyzer-Colorimeter
    Type: Dataset
    Format: text/tab-separated-values, 4019 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
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