In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-02-20)
Abstract:
Pyrophosphate (PPi), a byproduct of macromolecule biosynthesis is maintained at low levels by soluble inorganic pyrophosphatases (sPPase) found in all eukaryotes. In plants, H + -pumping pyrophosphatases (H + -PPase) convert the substantial energy present in PPi into an electrochemical gradient. We show here, that both cold- and heat stress sensitivity of fugu5 mutants lacking the major H + -PPase isoform AVP1 is correlated with reduced SUMOylation. In addition, we show that increased PPi concentrations interfere with SUMOylation in yeast and we provide evidence that SUMO activating E1-enzymes are inhibited by micromolar concentrations of PPi in a non-competitive manner. Taken together, our results do not only provide a mechanistic explanation for the beneficial effects of AVP1 overexpression in plants but they also highlight PPi as an important integrator of metabolism and stress tolerance.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.44213.001
DOI:
10.7554/eLife.44213.002
DOI:
10.7554/eLife.44213.008
DOI:
10.7554/eLife.44213.003
DOI:
10.7554/eLife.44213.004
DOI:
10.7554/eLife.44213.005
DOI:
10.7554/eLife.44213.006
DOI:
10.7554/eLife.44213.007
DOI:
10.7554/eLife.44213.009
DOI:
10.7554/eLife.44213.010
DOI:
10.7554/eLife.44213.011
DOI:
10.7554/eLife.44213.013
DOI:
10.7554/eLife.44213.012
DOI:
10.7554/eLife.44213.014
DOI:
10.7554/eLife.44213.015
DOI:
10.7554/eLife.44213.016
DOI:
10.7554/eLife.44213.017
DOI:
10.7554/eLife.44213.018
DOI:
10.7554/eLife.44213.023
DOI:
10.7554/eLife.44213.019
DOI:
10.7554/eLife.44213.020
DOI:
10.7554/eLife.44213.021
DOI:
10.7554/eLife.44213.022
DOI:
10.7554/eLife.44213.024
DOI:
10.7554/eLife.44213.025
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3
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