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  • 1
    Online Resource
    Online Resource
    Retracted : Ras–ERK1/2 signaling promotes the development of uveal melanoma by downregulating H3K14ac
    Wiley ; 2019
    In:  Journal of Cellular Physiology Vol. 234, No. 9 ( 2019-09), p. 16011-16020
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 234, No. 9 ( 2019-09), p. 16011-16020
    Abstract: Ras–extracellular signal‐regulated protein kinases 1 and 2 (ERK1/2) signaling has been proposed as the crucial regulators in the development of various cancers. Histone acetylation at H3 lysine 14 (H3K14ac) is closely associated with gene expression and DNA damage. However, whether H3K14ac participates in mediating Ras–ERK1/2‐induced cell proliferation and migration in uveal melanoma cells remains unknown. The purpose of this study is to investigate the effect of H3K14ac on Ras–ERK1/2 affected uveal melanoma cell phenotypes. MP65 cells were transfected with Ras WT and Ras G12V/T35S , the unloaded plasmid of pEGFP‐N1 served as a negative control. Protein levels of phosphorylated ERK1/2 Thr202 and H3K14ac were assessed by western blot assay. Cell viability, number of colonies, migration, and the downstream genes of ERK1/2 were analyzed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide, soft‐agar colony formation, transwell, and chromatin immunoprecipitation assays. HA‐tag vectors of CLR3 and TIP60 and the small interfering RNAs that specific for CLR3 and MDM2 were transfected into MP65 cells to uncover the effects of CLR3, TIP60, and MDM2 on Ras–ERK1/2 mediated H3K14ac expression and MP65 cell phenotypes. We found that, Ras–ERK1/2 decreased H3K14ac expression in MP65 cells, and H3K14ac significantly suppressed Ras–ERK1/2‐induced cell viability, colony formation, and migration in MP65 cells. Moreover, the transcription of CYR61 , IGFBP3 , WNT16B , NT5E , GDF15 , and CARD16 was regulated by H3K14ac. Additionally, CLR3 silence recovered H3K14ac expression and reversed the effect of Ras–ERK1/2 on MP65 cell proliferation, migration and the mRNAs of ERK1/2 downstream genes. Besides, Ras–ERK1/2 decreased H3K14ac expression by MDM2‐mediated TIP60 degradation. In conclusion, Ras–ERK1/2 promoted uveal melanoma cells growth and migration by downregulating H3K14ac via MDM2‐mediated TIP60 degradation.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v234.9
    DOI: 10.1002/jcp.28259
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478143-8
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Retracted : Ras‐PI3K‐AKT signaling promotes the occurrence and development of uveal melanoma by downregulating H3K56ac expression
    Wiley ; 2019
    In:  Journal of Cellular Physiology Vol. 234, No. 9 ( 2019-09), p. 16032-16042
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 234, No. 9 ( 2019-09), p. 16032-16042
    Abstract: Uveal melanoma (UM) is an intraocular malignant tumor characterized by rapid progression and recurrence. The current conventional treatments are unsatisfactory. Histone acetylation at H3 lysine 56 (H3K56ac) has been reported to be a tumor suppressor in breast cancer. However, whether H3K56ac prevents the occurrence and development of UM remains uninvestigated. The study aimed to explore the regulatory effect of H3K56ac on Ras‐PI3K‐AKT induced UM cells proliferation and migration. Methods The vectors of pEGFP‐Ras WT , pEGFP‐K‐Ras G12V/Y40C , and pEGFP‐N1 were transfected into MP46 cells, and protein levels of phosphorylated AKT Ser473 and H3K56ac were examined using western blot analysis. The effect of H3K56ac on cell proliferation and migration were studied using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5 diphenyl tetrazolium bromide, colony formation, and Transwell assays. Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and chromatin immunoprecipitation assays were performed to determine the phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) downstream genes. Further, the regulatory effects of silent mating type information regulation 2 homolog‐1 (SIRT1), general control nonderepressible 5 (GCN5), and mouse double minute 2 homolog (MDM2) on Ras‐PI3K‐AKT affected H3K56ac expression were also investigated. Results H3K56ac expression was specifically downregulated by Ras‐PI3K‐AKT activation pathway. H3K56ac inhibited the tumorigenic effect of Ras‐PI3K‐AKT on MP46 cells viability, colony formation, and migration, as well as participated in regulating the transcription of PI3K/AKT downstream genes. SIRT1 silence recovered H3K56ac expression, and reversed the tumorigenic effect of Ras‐PI3K‐AKT activation on MP46 cells. Downregulation of H3K56ac induced by Ras‐PI3K‐AKT activation was found to be associated with MDM2‐mediated the degradation of GCN5. Conclusions The results demonstrated that Ras‐PI3K‐AKT signaling promoted UM cells proliferation and migration via downregulation of H3K56ac expression, which might be related to MDM2‐mediated the degradation of GCN5.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v234.9
    DOI: 10.1002/jcp.28261
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1478143-8
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    N ‐Doped Nonalternant Nanoribbons with Excellent Nonlinear Optical Performance
    Wiley ; 2023
    In:  Angewandte Chemie International Edition Vol. 62, No. 33 ( 2023-08-14)
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 62, No. 33 ( 2023-08-14)
    Abstract: Two novel N ‐doped nonalternant nanoribbons ( NNNR‐1 and NNNR‐2 ) featuring multiple fused N ‐heterocycles and bulky solubilizing groups were prepared via bottom‐up solution synthesis. NNNR‐2 achieves a total molecular length of 33.8 Å, which represents the longest soluble N ‐doped nonalternant nanoribbon reported to date. The pentagon subunits and doping of N atoms in NNNR‐1 and NNNR‐2 have successfully regulated their electronic properties, achieving high electron affinity and good chemical stability enabled by the nonalternant conjugation and electronic effects. When applied a laser pulse of 532 nm, the 13‐rings nanoribbon NNNR‐2 shows outstanding nonlinear optical (NLO) responses, with the nonlinear extinction coefficient of 374 cm GW −1 , much higher than those of NNNR‐1 (96 cm GW −1 ) and the well‐known NLO material C 60 (153 cm GW −1 ). Our findings indicate that the N ‐doping of nonalternant nanoribbons is an effective strategy to access another type of excellent material system for high‐performance NLO applications, which can be extended to construct numerous heteroatom‐doped nonalternant nanoribbons with fine‐tunable electronic properties.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v62.33
    DOI: 10.1002/anie.202306418
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 4
    Online Resource
    Online Resource
    Genome‐wide association study for pigmentation traits in Chinese Holstein population
    Wiley ; 2014
    In:  Animal Genetics Vol. 45, No. 5 ( 2014-10), p. 740-744
    Details
    In: Animal Genetics, Wiley, Vol. 45, No. 5 ( 2014-10), p. 740-744
    Abstract: With the Illumina Bovine SNP 50K BeadChip, we performed a genome‐wide association study ( GWAS ) for two pigmentation traits in a Chinese Holstein population: proportion of black ( PB ) and teat colour ( TC ). A case–control design was used. Cases were the cows with PB 〈 0.30 ( n  =   129) and TC 〈 2 points ( n  =   140); controls were those with PB 〉 0.90 ( n  =   58) and TC 〉 4 points ( n  =   281). The RM test of roadtrips (version 1.2) was applied to detect SNP s for the two traits with 42 883 and 42 741 SNP s respectively. A total of nine and 12 genome‐wide significant ( P  〈   0.05) SNP s associated with PB and TC respectively were identified. Of these, two SNP s for PB were located within the KIT and IGFBP 7 genes, and the other four SNP s were 23~212 kb away from the PDGFRA gene on BTA 6; nine SNP s associated with TC were located within or 21~78.8 kb away from known genes on chromosomes 4, 11, 22, 23 and 24. By combing through our GWAS results and the biological functions of the genes, we suggest that the KIT , IGFBP 7 , PDGFRA , MITF , ING 3 and WNT 16 genes are promising candidates for PB and TC in Holstein cattle, providing a basis for further investigation on the genetic mechanism of pigmentation formation.
    Type of Medium: Online Resource
    ISSN: 0268-9146 , 1365-2052
    URL: Issue
    URL: Article
    DOI: 10.1111/age.2014.45.issue-5
    DOI: 10.1111/age.12189
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 1472889-8
    SSG: 22
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    KLF2 / PPARγ axis contributes to trauma‐induced heterotopic ossification by regulating mitochondrial dysfunction
    Wiley ; 2023
    In:  Cell Proliferation
    Details
    In: Cell Proliferation, Wiley
    Abstract: Trauma‐induced heterotopic ossification (HO) is a complex disorder after musculoskeletal injury and characterized by aberrant extraskeletal bone formation. Recent studies shed light on critical role of dysregulated osteogenic differentiation in aberrant bone formation. Krupel‐like factor 2 (KLF2) and peroxisome proliferator‐activated receptor gamma (PPARγ) are master adapter proteins that link cellular responses to osteogenesis; however, their roles and relationships in HO remain elusive. Using a murine burn/tenotomy model in vivo, we identified elevated KLF2 and reduced PPARγ levels in tendon stem/progenitor cells (TSPCs) during trauma‐induced HO formation. Both KLF2 inhibition and PPARγ promotion reduced mature HO, whereas the effects of PPARγ promotion were abolished by KLF2 overexpression. Additionally, mitochondrial dysfunction and reactive oxygen species (ROS) production also increased after burn/tenotomy, and improvements in mitochondrial function (ROS scavenger) could alleviate HO formation, but were abolished by KLF2 activation and PPARγ suppression by affecting redox balance. Furthermore, in vitro, we found increased KLF2 and decreased PPARγ levels in osteogenically induced TSPCs. Both KLF2 inhibition and PPARγ promotion relieved osteogenesis by improving mitochondrial function and maintaining redox balance, and effects of PPARγ promotion were abolished by KLF2 overexpression. Our findings suggest that KLF2/PPARγ axis exerts regulatory effects on trauma‐induced HO through modulation of mitochondrial dysfunction and ROS production in TSPCs by affecting redox balance. Targeting KLF2/PPARγ axis and mitochondrial dysfunction can represent attractive approaches to therapeutic intervention in trauma‐induced HO.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Article
    DOI: 10.1111/cpr.13521
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2019986-7
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Identification and characterization of select oxysterols as ligands for GPR17
    Wiley ; 2023
    In:  British Journal of Pharmacology Vol. 180, No. 4 ( 2023-02), p. 401-421
    Details
    In: British Journal of Pharmacology, Wiley, Vol. 180, No. 4 ( 2023-02), p. 401-421
    Abstract: G‐protein coupled receptor 17 (GPR17) is an orphan receptor involved in the process of myelination, due to its ability to inhibit the maturation of oligodendrocyte progenitor cells (OPCs) into myelinating oligodendrocytes. Despite multiple claims that the biological ligand has been identified, it remains an orphan receptor. Experimental Approach Seventy‐seven oxysterols were screened in a cell‐free [ 35 S]GTPγS binding assay using membranes from cells expressing GPR17. The positive hits were characterized using adenosine 3′,5′ cyclic monophosphate (cAMP), inositol monophosphate (IP1) and calcium mobilization assays, with results confirmed in rat primary oligodendrocytes. Rat and pig brain extracts were separated by high‐performance liquid chromatography (HPLC) and endogenous activator(s) were identified in receptor activation assays. Gene expression studies of GPR17 , and CYP46A1 (cytochrome P450 family 46 subfamily A member 1) enzymes responsible for the conversion of cholesterol into specific oxysterols, were performed using quantitative real‐time PCR. Key Results Five oxysterols were able to stimulate GPR17 activity, including the brain cholesterol, 24(S)‐hydroxycholesterol (24S‐HC). A specific brain fraction from rat and pig extracts containing 24S‐HC activates GPR17 in vitro. Expression of Gpr17 during mouse brain development correlates with the expression of Cyp46a1 and the levels of 24S‐HC itself. Other active oxysterols have low brain concentrations below effective ranges. Conclusions and Implications Oxysterols, including but not limited to 24S‐HC, could be physiological activators for GPR17 and thus potentially regulate OPC differentiation and myelination through activation of the receptor.
    Type of Medium: Online Resource
    ISSN: 0007-1188 , 1476-5381
    URL: Issue
    URL: Article
    DOI: 10.1111/bph.v180.4
    DOI: 10.1111/bph.15969
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2029728-2
    SSG: 15,3
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  • 7
    Online Resource
    Online Resource
    N ‐Doped Nonalternant Nanoribbons with Excellent Nonlinear Optical Performance
    Wiley ; 2023
    In:  Angewandte Chemie Vol. 135, No. 33 ( 2023-08-14)
    Details
    In: Angewandte Chemie, Wiley, Vol. 135, No. 33 ( 2023-08-14)
    Abstract: Two novel N ‐doped nonalternant nanoribbons ( NNNR‐1 and NNNR‐2 ) featuring multiple fused N ‐heterocycles and bulky solubilizing groups were prepared via bottom‐up solution synthesis. NNNR‐2 achieves a total molecular length of 33.8 Å, which represents the longest soluble N ‐doped nonalternant nanoribbon reported to date. The pentagon subunits and doping of N atoms in NNNR‐1 and NNNR‐2 have successfully regulated their electronic properties, achieving high electron affinity and good chemical stability enabled by the nonalternant conjugation and electronic effects. When applied a laser pulse of 532 nm, the 13‐rings nanoribbon NNNR‐2 shows outstanding nonlinear optical (NLO) responses, with the nonlinear extinction coefficient of 374 cm GW −1 , much higher than those of NNNR‐1 (96 cm GW −1 ) and the well‐known NLO material C 60 (153 cm GW −1 ). Our findings indicate that the N ‐doping of nonalternant nanoribbons is an effective strategy to access another type of excellent material system for high‐performance NLO applications, which can be extended to construct numerous heteroatom‐doped nonalternant nanoribbons with fine‐tunable electronic properties.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v135.33
    DOI: 10.1002/ange.202306418
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 8
    Online Resource
    Online Resource
    The Influence of Xanthan Gum on Rheological Properties and In Vitro Digestibility of Kudzu ( Pueraria lobata ) Starch
    Wiley ; 2020
    In:  Starch - Stärke Vol. 72, No. 3-4 ( 2020-03)
    Details
    In: Starch - Stärke, Wiley, Vol. 72, No. 3-4 ( 2020-03)
    Abstract: The study describes the effects of xanthan gum (0.5–2.0%) on the in vitro digestibility and rheological and pasting properties of kudzu ( Pueraria lobata ) starch pastes. The flow behavior index ( n ) shows a decrease in trends (from 0.43 to 0.31) as the xanthan gum concentration increases, indicating pseudoplastic behavior. Dynamic viscoelasticity analysis indicate that the elastic ( G ′) and viscous ( G ″) moduli increase in the presence of xanthan gum, suggesting a strong interaction between xanthan gum and kudzu starch in the composite system. All concentrations of xanthan gum result in increased elasticity ( G ′  〉   G ″). Pasting properties reveal that the peak and final viscosity of the xanthan gum–kudzu starch dispersion decreases with the increasing concentration of xanthan gum, highlighting the importance of xanthan gum in controlling overall viscosity. The addition of xanthan gum also decreases the hydrolysis of kudzu starch during in vitro digestion.
    Type of Medium: Online Resource
    ISSN: 0038-9056 , 1521-379X
    URL: Issue
    URL: Article
    DOI: 10.1002/star.v72.3-4
    DOI: 10.1002/star.201900139
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1481133-9
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  • 9
    Online Resource
    Online Resource
    Non‐Hermitian Topolectrical Circuit Sensor with High Sensitivity
    Wiley ; 2023
    In:  Advanced Science Vol. 10, No. 19 ( 2023-07)
    Details
    In: Advanced Science, Wiley, Vol. 10, No. 19 ( 2023-07)
    Abstract: Electronic sensors play important roles in various applications, such as industry and environmental monitoring, biomedical sample ingredient analysis, wireless networks and so on. However, the sensitivity and robustness of current schemes are often limited by the low quality‐factors of resonators and fabrication disorders. Hence, exploring new mechanisms of the electronic sensor with a high‐level sensitivity and a strong robustness is of great significance. Here, a new way to design electronic sensors with superior performances based on exotic properties of non‐Hermitian topological physics is proposed. Owing to the extreme boundary‐sensitivity of non‐Hermitian topological zero modes, the frequency shift induced by boundary perturbations can show an exponential growth trend with respect to the size of non‐Hermitian topolectrical circuit sensors. Moreover, such an exponential growth sensitivity is also robust against disorders of circuit elements. Using designed non‐Hermitian topolectrical circuit sensors, the ultrasensitive identification of the distance, rotation angle, and liquid level is further experimentally verified with the designed capacitive devices. The proposed non‐Hermitian topolectrical circuit sensors can possess a wide range of applications in ultrasensitive environmental monitoring and show an exciting prospect for next‐generation sensing technologies.
    Type of Medium: Online Resource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v10.19
    DOI: 10.1002/advs.202301128
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2808093-2
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  • 10
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    Online Resource
    Mineral‐geochemical analysis on the Danzhou tungsten‐cesium polymetallic deposit, Hainan Province
    Wiley ; 2017
    In:  Acta Geologica Sinica - English Edition Vol. 91, No. s1 ( 2017-05), p. 204-205
    Details
    In: Acta Geologica Sinica - English Edition, Wiley, Vol. 91, No. s1 ( 2017-05), p. 204-205
    Type of Medium: Online Resource
    ISSN: 1000-9515 , 1755-6724
    URL: Issue
    URL: Article
    DOI: 10.1111/acgs.2017.91.issue-s1
    DOI: 10.1111/1755-6724.13257
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2420386-5
    SSG: 6,25
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