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  • 1
    Electronic Resource
    Electronic Resource
    Catabolite repression in Escherichia coli mutants lacking cyclic AMP (1978)
    Springer
    Molecular genetics and genomics 162 (1978), S. 83-87 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The regulation of catabolite repression of β-galactosidase has been studied in Escherichia coli mutants deleted for the adenyl cyclase gene (cya Δ), and thus unable to synthesize cyclic AMP. It has been found that, provided a second mutation occurs either in the crp gene coding for the catabolite gene activator protein (CAP) or in the Lactose region, these mutants exhibit catabolite repression. If the catabolite repression seen in the mutant strains corresponds to the mechanism operating in wild-type cells, the results would suggest that the intracellular concentration of cyclic AMP cannot be the unique regulator of catabolite repression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333853
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of urea on catabolite sensitive operons in Escherichia coli K 12 (1980)
    Springer
    Molecular genetics and genomics 178 (1980), S. 611-616 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Low concentrations of urea specifically inhibit the expression of catabolite sensitive genes (the lactose, galactose and maltose operons and the tryptophanase gene). This inhibition depends upon growth conditions, i.e. carbon source and temperature. The main effect of urea is exerted at the level of transcription initiation. However an additional inhibitory effect is observed on the decay and expression of the β-galactosidase messenger. In a strain harboring the UV5 mutation in the lactose promoter, the effect at the level of transcription is relieved while the effect on the decay and the expression of the β-galactosidase messenger remains the same. Just like the extreme physiological catabolite repression, the urea effect occurs even in a cyaΔ strain and is not antagonized by addition of adenosine 3′–5′ cyclic monophosphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337868
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  • 3
    Electronic Resource
    Electronic Resource
    Catabolite modulator factor: Physiological properties and in vivo effects (1978)
    Springer
    Molecular genetics and genomics 162 (1978), S. 89-94 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Catabolite modulator factor (CMF) specifically inhibits the expression of operons sensitive to catabolite repression. Systems known to be catabolite independent are not affected by CMF. The rate of metabolism of CMF depends on the extent of catabolite repression: it is slow under conditions of strong repression and high in catabolically derepressed cells. Cyclic AMP does not interfere with the rate of CMF metabolism. It has been found that a certain class of crp mutants are partially resistant to the repressive effect of CMF. Our results provide considerable support for the existence of an additional negative control in the regulation of catabolite repression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333854
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Two different mechanisms for urea action at the LAC and TNA operons in Escherichia coli (1990)
    Springer
    Molecular genetics and genomics 220 (1990), S. 419-424 
    Details
    ISSN: 1617-4623
    Keywords: Catabolite repression ; Catabolite gene activator ; Transcription initiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Urea, at concentrations which do not interfere with bacterial growth, specifically inhibits the expression of catabolite sensitive operons. To search for the target and the mechanism of urea action we measured lactose (lac) and tryptophanase (tna) specific mRNA synthesis in vivo and in vitro. We show that urea acts by two different mechanisms at these two catabolite sensitive operons, resembling the manner in which catabolite repression regulates lac and tna. At the lac promoter, urea abolishes transcription initiation or blocks an early step in mRNA elongation without interfering with the binding of RNA polymerase and catabolite gene activator protein (CAP). At the tna promoter, urea does not abolish transcription initiation but could interfere with tnaC translation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391748
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  • 5
    Electronic Resource
    Electronic Resource
    Multiple regulation of the activity of adenylate cyclase in Escherichia coli (1982)
    Springer
    Molecular genetics and genomics 185 (1982), S. 262-268 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have studied the correlation between the activities of adenylate cyclase (ATP pyrophosphatelyase-(cyclizing); EC 4.6.1.1) and in vivo rates of synthesis and intracellular concentrations of adenosine 3′,5′ cyclic monophosphate (cAMP) under various growth conditions in wild-type Escherichia coli and in mutants lacking or overproducing the cAMP receptor protein (CAP). We showed that when wild-type bacteria are grown in the presence of a variety of carbon sources the intracellular concentrations of cAMP are inversely related to the adenylate cyclase activities determined in permeabilized cells, suggesting that the carbon source-dependent modulation of cAMP levels is not directly related to the regulation of adenylate cyclase activity. In mutants lacking functional CAP (crp) the in vivo rates of cAMP synthesis are several hundred-fold higher than in the wild-type parent without a parallel increase of adenylate cyclase activities. In a strain carrying multiple copies of the crp gene and overproducing CAP the activity of adenylate cyclase is severely inhibited, although the in vivo rate of cAMP synthesis is similar to the parental strain. We interpret these results as indicating that CAP controls mainly the activity rather than the synthesis of adenylate cyclase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330796
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  • 6
    Electronic Resource
    Electronic Resource
    Transcriptional control of polarity in Escherichia coli by cAMP (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 96-100 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Escherichia coli, 3′5′-adenosine cyclic monophosphate (cAMP) and its receptor protein (CAP) are known to be involved in the control of transcription initiation of catabolic operons. In previous papers we have shown that the cAMP-CAP complex is also involved as a modulator of polarity in polycistronic transcription units. Furthermore we showed that there exists a functional relationship between this complex and the transcription termination protein, Rho. In this work, we measured mRNA synthesis corresponding to the promoter proximal and distal parts of the lac and gal operons by DNA-RNA hybridization. We show that in these operons the main polarity effect is essentially transcriptional and the cAMP-CAP complex decreases polarity by interfering with premature transcription termination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332730
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    Paper (German National Licenses)
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